2024_Effect Of Aquilaria Malaccensis Aqueous Extract On Reproductive Organs, Sperm Quality And Testosterone Concentration Of Male Sprague Dawley

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date 2024-09-08
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originalfilename EFFECT OF Aquilaria malaccensis AQUEOUS EXTRACT ON REPRODUCTIVE ORGANS, SPERM QUALITY AND TESTOSTERONE CONCENTRATION OF MALE SPRAGUE DAWLEY (MASTER_2024).pdf
person Norahidah Zaidi
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spelling 17327 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17327 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.6 134 Microsoft® Word 2016 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin Copyright©PWB2025 Dissertations, Academic 2024-09-08 Aquilaria malaccensis Phytochemical screening Sprague Dawley rats Norahidah Zaidi Karas Male reproductive system Sperm quality Testosterone Herbal extract Reproductive toxicity Andrology Aquilaria malaccensis Medicinal plants—Physiological effect Herbs—Therapeutic use Rats—Physiology Reproductive toxicology Spermatozoa—Analysis Testosterone—Physiological effect Fertility, Male—Effect of chemicals on 2024_Effect Of Aquilaria Malaccensis Aqueous Extract On Reproductive Organs, Sperm Quality And Testosterone Concentration Of Male Sprague Dawley The use of herbal plants as supplements or therapies for various medical issues has recently attracted a lot of interest in Malaysia. Men’s dietary and nutritional practices can influence their reproductive health status. Aquilaria malaccensis, locally known as karas, has gained prominence due to its pharmacological characteristics, which has the capability to treat various diseases. However, the study on the effects of A. malaccensis on male reproductive function and fertility are very limited, especially on sperm quality and reproductive hormone. The purpose of this study was to determine the nutritional content of A. malaccensis and its effect on the weight of reproductive organs (testis, epididymis, prostate gland, seminal vesicle), seminiferous tubular diameter and epithelial height, sperm quality (sperm motility, sperm morphology, sperm concentration) and the testosterone hormone level in the male of Sprague Dawley rats. The nutritional content of A. malaccensis was assessed using proximate analysis and phytochemical screening. A total of 24 male Sprague Dawley rats (n=24) were categorized into four A. malaccensis treatment groups; Control (C: 0 g/kg body weight, n=6), Treatment 1 (T1: 1 g/kg body weight, n=6), Treatment 2 (T2: 2 g/kg body weight, n=6) and Treatment 3 (T3: 3 g/kg body weight, n=6), respectively. A. malaccensis extract was administered orally via oral gavage once daily for 28 days. The rats were sacrificed on Day 29 for assessment on their reproductive function. Data from proximate analysis showed that A. malaccensis consists of moisture content (27.49%), ash (2.45%), crude protein (8.66%), crude fat (1.27%), crude fibre (22.15%), and carbohydrates (37.99%). The phytochemical constituents indicated the presence of tannins, saponins, glycosides, flavonoids, terpenoids, steroids, and alkaloids in A. malaccensis leaves extract. Significant decrease in the percentage of abnormal sperm (8.17%) and increase in sperm concentration (1.36 × 10⁻¹¹) were observed in T1 group as compared to C group. Increasing dosage of A. malaccensis seemed to increase the percentage of abnormal sperm and decrease the concentration of the sperm. The testosterone level in the T1 group was also significantly higher (1.18 ng/mL) as compared to C and T2 groups. A significant negative correlation was observed between sperm concentration and sperm morphology. In conclusion, the doses of 1, 2, and 3 g/kg of A. malaccensis did not affect the weight of reproductive organs, seminiferous tubules of the testis and sperm motility. On the other hand, the supplementation of A. malaccensis at 1 g/kg potentially yields beneficial effects on the male reproductive system by improving the percentage of normal sperm, sperm concentration and testosterone level. However, caution was advised when considering higher concentrations (2 and 3 g/kg) of A. malaccensis, as they may adversely affect male reproductive function by reducing the percentage of normal sperm, sperm concentration and testosterone level. uuid:aa3461b5-32b3-413a-8875-812f61e9f304 EFFECT OF Aquilaria malaccensis AQUEOUS EXTRACT ON REPRODUCTIVE ORGANS, SPERM QUALITY AND TESTOSTERONE CONCENTRATION OF MALE SPRAGUE DAWLEY (MASTER_2024).pdf Thesis
spellingShingle 2024_Effect Of Aquilaria Malaccensis Aqueous Extract On Reproductive Organs, Sperm Quality And Testosterone Concentration Of Male Sprague Dawley
state Terengganu
subject Dissertations, Academic
Aquilaria malaccensis
Medicinal plants—Physiological effect
Herbs—Therapeutic use
Rats—Physiology
Reproductive toxicology
Spermatozoa—Analysis
Testosterone—Physiological effect
Fertility, Male—Effect of chemicals on
summary The use of herbal plants as supplements or therapies for various medical issues has recently attracted a lot of interest in Malaysia. Men’s dietary and nutritional practices can influence their reproductive health status. Aquilaria malaccensis, locally known as karas, has gained prominence due to its pharmacological characteristics, which has the capability to treat various diseases. However, the study on the effects of A. malaccensis on male reproductive function and fertility are very limited, especially on sperm quality and reproductive hormone. The purpose of this study was to determine the nutritional content of A. malaccensis and its effect on the weight of reproductive organs (testis, epididymis, prostate gland, seminal vesicle), seminiferous tubular diameter and epithelial height, sperm quality (sperm motility, sperm morphology, sperm concentration) and the testosterone hormone level in the male of Sprague Dawley rats. The nutritional content of A. malaccensis was assessed using proximate analysis and phytochemical screening. A total of 24 male Sprague Dawley rats (n=24) were categorized into four A. malaccensis treatment groups; Control (C: 0 g/kg body weight, n=6), Treatment 1 (T1: 1 g/kg body weight, n=6), Treatment 2 (T2: 2 g/kg body weight, n=6) and Treatment 3 (T3: 3 g/kg body weight, n=6), respectively. A. malaccensis extract was administered orally via oral gavage once daily for 28 days. The rats were sacrificed on Day 29 for assessment on their reproductive function. Data from proximate analysis showed that A. malaccensis consists of moisture content (27.49%), ash (2.45%), crude protein (8.66%), crude fat (1.27%), crude fibre (22.15%), and carbohydrates (37.99%). The phytochemical constituents indicated the presence of tannins, saponins, glycosides, flavonoids, terpenoids, steroids, and alkaloids in A. malaccensis leaves extract. Significant decrease in the percentage of abnormal sperm (8.17%) and increase in sperm concentration (1.36 × 10⁻¹¹) were observed in T1 group as compared to C group. Increasing dosage of A. malaccensis seemed to increase the percentage of abnormal sperm and decrease the concentration of the sperm. The testosterone level in the T1 group was also significantly higher (1.18 ng/mL) as compared to C and T2 groups. A significant negative correlation was observed between sperm concentration and sperm morphology. In conclusion, the doses of 1, 2, and 3 g/kg of A. malaccensis did not affect the weight of reproductive organs, seminiferous tubules of the testis and sperm motility. On the other hand, the supplementation of A. malaccensis at 1 g/kg potentially yields beneficial effects on the male reproductive system by improving the percentage of normal sperm, sperm concentration and testosterone level. However, caution was advised when considering higher concentrations (2 and 3 g/kg) of A. malaccensis, as they may adversely affect male reproductive function by reducing the percentage of normal sperm, sperm concentration and testosterone level.
title 2024_Effect Of Aquilaria Malaccensis Aqueous Extract On Reproductive Organs, Sperm Quality And Testosterone Concentration Of Male Sprague Dawley
title_full 2024_Effect Of Aquilaria Malaccensis Aqueous Extract On Reproductive Organs, Sperm Quality And Testosterone Concentration Of Male Sprague Dawley
title_fullStr 2024_Effect Of Aquilaria Malaccensis Aqueous Extract On Reproductive Organs, Sperm Quality And Testosterone Concentration Of Male Sprague Dawley
title_full_unstemmed 2024_Effect Of Aquilaria Malaccensis Aqueous Extract On Reproductive Organs, Sperm Quality And Testosterone Concentration Of Male Sprague Dawley
title_short 2024_Effect Of Aquilaria Malaccensis Aqueous Extract On Reproductive Organs, Sperm Quality And Testosterone Concentration Of Male Sprague Dawley
title_sort 2024_effect of aquilaria malaccensis aqueous extract on reproductive organs, sperm quality and testosterone concentration of male sprague dawley