2024_Allelopathic Effects Of Root Exudates And Rhizosphere Soil From Turnera Subulata
| Format: | General Document |
|---|
| _version_ | 1860798335872401408 |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2024-09-10 |
| format | General Document |
| id | 17326 |
| institution | UniSZA |
| originalfilename | 17326_77d08c1b4f49f3a.pdf |
| person | Wan Zateel Aieeda Wan Abdul Halim |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17326 |
| sourcemedia | Server storage Scanned document |
| spelling | 17326 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17326 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.7 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin 169 Copyright©PWB2025 Allelopathy Turnera subulata Dissertations, Academic Microsoft® Word LTSC Wan Zateel Aieeda Wan Abdul Halim Root exudates Rhizosphere soil Allelochemicals LCMS analysis Bioassay species Weed management Allelopathic activity Plant physiological responses Plant-soil relationships Weeds—Control Allelopathy—Turnera subulata—Root exudates Liquid chromatography—Mass spectrometry Biochemical markers—Phenolic compounds Plant physiology 2024_Allelopathic Effects Of Root Exudates And Rhizosphere Soil From Turnera Subulata 2024-09-10 The overuse of herbicides for weed control eventually leads to the evolution of herbicide-resistant weeds as well as the impacts upon human health. Thus, the use of herbicides can be minimized by implementing allelopathy in agriculture. To date, limited studies had been conducted using medicinal plant species as potential allelopathic sources, especially Turnera subulata. To address this gap, this study was conducted to evaluate the allelopathic potential of root exudates and rhizosphere soil under T. subulata and to determine the allelopathic activity of soil and root aqueous extract under T. subulata on selected bioassay species and to identify the allelochemicals in root of T. subulata. T. subulata root and rhizosphere soil were used as the donor plant, whilst mustard (Brassica chinensis), wheat grass (Triticum aestivum), rice variety (MR303) and weedy rice (Oryza sativa var. sativa) served as the bioassay species in this study. The plant box method and rhizosphere soil assay method were carried out to evaluate the allelopathic potential from T. subulata. Further, the growth, physiological and biochemical responses of bioassay species were tested with T. subulata aqueous root and soil extract in different concentrations under laboratory and greenhouse conditions. Finally, the potential allelochemicals from T. subulata root were identified using Liquid Chromatography Mass Spectrometry (LCMS) analysis. The radicle length of B. chinensis was inhibited by 89.20% compared to the control in the plant box method. The effect of T. subulata root exudates on the radicle length of T. aestivum showed the highest linear regression coefficient (R² = 0.79) compared to other species. In the rhizosphere soil assay method, the radicle length of MR303 was observed with the highest inhibitory effect (28.57% compared to control) at 50 mg rhizosphere soil of T. subulata. Complete inhibition of growth was exhibited by B. chinensis and O. sativa when applied with 50 mg/mL aqueous root extract under laboratory conditions. Proline, malondialdehyde and hydrogen peroxide of B. chinensis significantly increased by 88.56%, 90.11% and 96.26%, respectively, compared to control when applied with 50 mg/mL aqueous root extract under greenhouse conditions. Meanwhile, chlorophyll content of O. sativa demonstrated the highest percentage of reduction (70.47%), followed by B. chinensis (66.06%), MR303 (55.71%) and T. aestivum (48.46%) compared to control at similar concentration. In this study, 19 potential allelochemical compounds were identified using LCMS analysis. The highest peak area of compounds detected was Esculin, with retention time (Rt) value 2.96. The results suggested that T. subulata root exudate and rhizosphere soil had allelopathic potential on the growth of selected bioassay species. T. subulata aqueous root and rhizosphere soil extract significantly inhibited the growth, physiological and biochemical parameters of bioassay species. Esculin, which is a phenolic compound, might confer this allelopathic activity. This discovery provides scientific evidence for the research and development of eco-friendly weed management in the future by utilizing T. subulata. uuid:1B0455BE-66AD-465F-AF50-EFD196A203B9 17326_77d08c1b4f49f3a.pdf Thesis |
| spellingShingle | 2024_Allelopathic Effects Of Root Exudates And Rhizosphere Soil From Turnera Subulata |
| state | Terengganu |
| subject | Dissertations, Academic Plant-soil relationships Weeds—Control Allelopathy—Turnera subulata—Root exudates Liquid chromatography—Mass spectrometry Biochemical markers—Phenolic compounds Plant physiology |
| summary | The overuse of herbicides for weed control eventually leads to the evolution of herbicide-resistant weeds as well as the impacts upon human health. Thus, the use of herbicides can be minimized by implementing allelopathy in agriculture. To date, limited studies had been conducted using medicinal plant species as potential allelopathic sources, especially Turnera subulata. To address this gap, this study was conducted to evaluate the allelopathic potential of root exudates and rhizosphere soil under T. subulata and to determine the allelopathic activity of soil and root aqueous extract under T. subulata on selected bioassay species and to identify the allelochemicals in root of T. subulata. T. subulata root and rhizosphere soil were used as the donor plant, whilst mustard (Brassica chinensis), wheat grass (Triticum aestivum), rice variety (MR303) and weedy rice (Oryza sativa var. sativa) served as the bioassay species in this study. The plant box method and rhizosphere soil assay method were carried out to evaluate the allelopathic potential from T. subulata. Further, the growth, physiological and biochemical responses of bioassay species were tested with T. subulata aqueous root and soil extract in different concentrations under laboratory and greenhouse conditions. Finally, the potential allelochemicals from T. subulata root were identified using Liquid Chromatography Mass Spectrometry (LCMS) analysis. The radicle length of B. chinensis was inhibited by 89.20% compared to the control in the plant box method. The effect of T. subulata root exudates on the radicle length of T. aestivum showed the highest linear regression coefficient (R² = 0.79) compared to other species. In the rhizosphere soil assay method, the radicle length of MR303 was observed with the highest inhibitory effect (28.57% compared to control) at 50 mg rhizosphere soil of T. subulata. Complete inhibition of growth was exhibited by B. chinensis and O. sativa when applied with 50 mg/mL aqueous root extract under laboratory conditions. Proline, malondialdehyde and hydrogen peroxide of B. chinensis significantly increased by 88.56%, 90.11% and 96.26%, respectively, compared to control when applied with 50 mg/mL aqueous root extract under greenhouse conditions. Meanwhile, chlorophyll content of O. sativa demonstrated the highest percentage of reduction (70.47%), followed by B. chinensis (66.06%), MR303 (55.71%) and T. aestivum (48.46%) compared to control at similar concentration. In this study, 19 potential allelochemical compounds were identified using LCMS analysis. The highest peak area of compounds detected was Esculin, with retention time (Rt) value 2.96. The results suggested that T. subulata root exudate and rhizosphere soil had allelopathic potential on the growth of selected bioassay species. T. subulata aqueous root and rhizosphere soil extract significantly inhibited the growth, physiological and biochemical parameters of bioassay species. Esculin, which is a phenolic compound, might confer this allelopathic activity. This discovery provides scientific evidence for the research and development of eco-friendly weed management in the future by utilizing T. subulata. |
| title | 2024_Allelopathic Effects Of Root Exudates And Rhizosphere Soil From Turnera Subulata |
| title_full | 2024_Allelopathic Effects Of Root Exudates And Rhizosphere Soil From Turnera Subulata |
| title_fullStr | 2024_Allelopathic Effects Of Root Exudates And Rhizosphere Soil From Turnera Subulata |
| title_full_unstemmed | 2024_Allelopathic Effects Of Root Exudates And Rhizosphere Soil From Turnera Subulata |
| title_short | 2024_Allelopathic Effects Of Root Exudates And Rhizosphere Soil From Turnera Subulata |
| title_sort | 2024_allelopathic effects of root exudates and rhizosphere soil from turnera subulata |