2024_The Potential Effects Of Malaysian Kelulut Honey On Enteric And Respiratory Infections By Using Wistar Rats Model
| Format: | General Document |
|---|
| _version_ | 1860798327197532160 |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2025-10-15 11:46 |
| format | General Document |
| id | 17222 |
| institution | UniSZA |
| originalfilename | THE POTENTIAL EFFECTS OF MALAYSIAN KELULUT HONEY ON ENTERIC AND RESPIRATORY INFECTIONS BY USING WISTAR RATS MODEL (PHD_2024).pdf |
| person | Ibrahim Khaled M Alkafaween |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17222 |
| sourcemedia | Server storage Scanned document |
| spelling | 17222 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17222 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Health Sciences English application/pdf 1.7 179 iLovePDF Server storage Scanned document UniSZA Private Access UniSZA Copyright©PWB2025 UniSZA Dissertations-Academic Ibrahim Khaled M Alkafaween Honey – Therapeutic Use – Malaysia Bees, Stingless – Physiology – Malaysia Bacterial Infections – Treatment – Malaysia Anti-inflammatory Agents – Research – Malaysia Kelulut Honey Stingless Bee Honey Enteric Infections Respiratory Infections Wistar Rats Antimicrobial Activity Inflammatory Response Animal Model Traditional Medicine Natural Product Pharmacology 2024_The Potential Effects Of Malaysian Kelulut Honey On Enteric And Respiratory Infections By Using Wistar Rats Model The prevalence of respiratory and enteric infections underscores the severity of these diseases. Respiratory and enteric infections, often caused by Escherichia coli and Staphylococcus aureus,pose significant health challenges. Antibiotics, while essential for treatment, are increasingly ineffective due to bacterial resistance. However, there is hope in the form of Malaysian Kelulut Honey (MKH), which has demonstrated potential in treating wounds and infections. This study delves into the promising effects of MKH on S. aureus and E. coli infections in Wistar rats, examining its influence on microbiological, blood, and molecular parameters. Forty male Wistar rats (3 months old, 200-225 g) were subjected to a 12-hour light-dark cycle and divided into eight groups of five. The positive control group received MKH orally (5 g/kg body weight). The negative control group received water. Two untreated groups were injected with S. aureus or E. coli for respiratory or enteric infections. Two treatment groups were injected with E. coli or S. aureus and then treated with MKH for three weeks. Preventive groups received MKH orally for one week before infection. All rats were monitored before lung and intestine tissues' biochemical and gene expression analyses. MKH significantly stimulated the immune system and reduced inflammation by boosting antioxidants. Total bacterial colony forming units (CFUs) and biomarkers for liver and kidney function decreased significantly (P<0.05) in both preventive and treatment groups. Antioxidant biomarkers increased notably (P<0.05), suggesting MKH enhances antioxidant defenses. Immunoglobulin G (IgG) levels increased significantly (P<0.05) for both infection types in preventive and treatment groups, indicating improved immune response. Interferon-gamma (IFN-γ) levels decreased in the preventive group. They increased in the treatment group for respiratory infections, while both groups showed decreased levels for enteric infections, indicating varying regulatory mechanisms.Interleukin 8 (IL-8) levels increased in both preventive and treatment groups for enteric infections. Still, they decreased in the preventive group and increased in the treatment group for respiratory infections, suggesting MKH modulates inflammation contextually. Gene expression analysis revealed that MKH increased antioxidant gene expression and alpha-2 macroglobulin (α2-MG), while tumor necrosis factor-alpha (TNF-α) expression was downregulated. In contrast, in infected groups, alpha-1 acid glycoprotein (AGP) and Glutathione S-transferases (GSTs) were upregulated, indicating MKH‘s role in modulating inflammation and enhancing detoxification. MKH effectively reduces bacterial CFUs, acts as a potent antioxidant, and mitigates immunosuppressive and inflammatory changes, as well as hepatic and renal toxicity induced by infections. It regulates antioxidant agents, acute phase proteins, and tissue cytokine gene expression. The potential of MKH in treating human intestinal and respiratory diseases is a beacon of hope. However, the urgency and importance of future clinical trials to further explore its efficacy and safety cannot be overstated. 2025-10-15 11:46 uuid:e6f48ac5-04d8-4aee-bc81-71a7c34dc87d THE POTENTIAL EFFECTS OF MALAYSIAN KELULUT HONEY ON ENTERIC AND RESPIRATORY INFECTIONS BY USING WISTAR RATS MODEL (PHD_2024).pdf Thesis |
| spellingShingle | 2024_The Potential Effects Of Malaysian Kelulut Honey On Enteric And Respiratory Infections By Using Wistar Rats Model |
| state | Terengganu |
| subject | Dissertations-Academic Honey – Therapeutic Use – Malaysia Bees, Stingless – Physiology – Malaysia Bacterial Infections – Treatment – Malaysia Anti-inflammatory Agents – Research – Malaysia |
| summary | The prevalence of respiratory and enteric infections underscores the severity of these diseases. Respiratory and enteric infections, often caused by Escherichia coli and Staphylococcus aureus,pose significant health challenges. Antibiotics, while essential for treatment, are increasingly ineffective due to bacterial resistance. However, there is hope in the form of Malaysian Kelulut Honey (MKH), which has demonstrated potential in treating wounds and infections. This study delves into the promising effects of MKH on S. aureus and E. coli infections in Wistar rats, examining its influence on microbiological, blood, and molecular parameters. Forty male Wistar rats (3 months old, 200-225 g) were subjected to a 12-hour light-dark cycle and divided into eight groups of five. The positive control group received MKH orally (5 g/kg body weight). The negative control group received water. Two untreated groups were injected with S. aureus or E. coli for respiratory or enteric infections. Two treatment groups were injected with E. coli or S. aureus and then treated with MKH for three weeks. Preventive groups received MKH orally for one week before infection. All rats were monitored before lung and intestine tissues' biochemical and gene expression analyses. MKH significantly stimulated the immune system and reduced inflammation by boosting antioxidants. Total bacterial colony forming units (CFUs) and biomarkers for liver and kidney function decreased significantly (P<0.05) in both preventive and treatment groups. Antioxidant biomarkers increased notably (P<0.05), suggesting MKH enhances antioxidant defenses. Immunoglobulin G (IgG) levels increased significantly (P<0.05) for both infection types in preventive and treatment groups, indicating improved immune response. Interferon-gamma (IFN-γ) levels decreased in the preventive group. They increased in the treatment group for respiratory infections, while both groups showed decreased levels for enteric infections, indicating varying regulatory mechanisms.Interleukin 8 (IL-8) levels increased in both preventive and treatment groups for enteric infections. Still, they decreased in the preventive group and increased in the treatment group for respiratory infections, suggesting MKH modulates inflammation contextually. Gene expression analysis revealed that MKH increased antioxidant gene expression and alpha-2 macroglobulin (α2-MG), while tumor necrosis factor-alpha (TNF-α) expression was downregulated. In contrast, in infected groups, alpha-1 acid glycoprotein (AGP) and Glutathione S-transferases (GSTs) were upregulated, indicating MKH‘s role in modulating inflammation and enhancing detoxification. MKH effectively reduces bacterial CFUs, acts as a potent antioxidant, and mitigates immunosuppressive and inflammatory changes, as well as hepatic and renal toxicity induced by infections. It regulates antioxidant agents, acute phase proteins, and tissue cytokine gene expression. The potential of MKH in treating human intestinal and respiratory diseases is a beacon of hope. However, the urgency and importance of future clinical trials to further explore its efficacy and safety cannot be overstated. |
| title | 2024_The Potential Effects Of Malaysian Kelulut Honey On Enteric And Respiratory Infections By Using Wistar Rats Model |
| title_full | 2024_The Potential Effects Of Malaysian Kelulut Honey On Enteric And Respiratory Infections By Using Wistar Rats Model |
| title_fullStr | 2024_The Potential Effects Of Malaysian Kelulut Honey On Enteric And Respiratory Infections By Using Wistar Rats Model |
| title_full_unstemmed | 2024_The Potential Effects Of Malaysian Kelulut Honey On Enteric And Respiratory Infections By Using Wistar Rats Model |
| title_short | 2024_The Potential Effects Of Malaysian Kelulut Honey On Enteric And Respiratory Infections By Using Wistar Rats Model |
| title_sort | 2024_the potential effects of malaysian kelulut honey on enteric and respiratory infections by using wistar rats model |