2025_Interaction Of Probiotic Lactobacillus Species With Periodontal Pathogens On Biofilm Degradation And Quorum Sensing Activity
| Format: | General Document |
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| _version_ | 1860798326749790208 |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2025-03-24 08:58 |
| format | General Document |
| id | 17220 |
| institution | UniSZA |
| originalfilename | INTERACTION OF PROBIOTIC LACTOBACILLUS SPECIES WITH PERIODONTAL PATHOGENS ON BIOFILM DEGRADATION AND QUORUM SENSING ACTIVITY.pdf |
| person | Haswati @ Nurhayati Binti Abdullah |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17220 |
| sourcemedia | Server storage Scanned document |
| spelling | 17220 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17220 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Health Sciences English application/pdf 1.7 148 Microsoft® Word for Microsoft 365 Server storage Scanned document UniSZA Private Access UniSZA Copyright©PWB2025 UniSZA Haswati @ Nurhayati Binti Abdullah Lactobacillus Probiotik Patogen Periodontal Periodontitis Lactobacillus — Physiology. Dissertations-Academic Probiotics — Therapeutic use Biofilms — Degradation Quorum sensing — Inhibition Biofilem Komunikasi bakteria Biofilms formed by periodontal pathogens contribute to chronic inflammation, leading to damage of connective tissues, gingival epithelium, and the alveolar bone supporting teeth. The removal of these biofilms using antibiotics is often challenging due to their limited penetration. Probiotics, such as Lactobacillus, have the potential to improve dental health by modulating the oral microbiome. Since quorum sensing (QS) plays a key role in biofilm formation, probiotic Lactobacillus may interfere with QS mechanisms, thereby influencing biofilm development. However, the underlying biochemical pathways by which probiotics affect periodontal infections remain poorly understood. This study aims to examine the biofilm formation and QS activity of Aggregatibacter actinomycetemcomitans and Pseudomonas aeruginosa, focusing on the influence of probiotic Lactobacillus strains. The antibacterial activity of probiotic Lactobacillus against A. actinomycetemcomitans and P. aeruginosa was evaluated using the spot-on-lawn method. The biofilm degradation potential of probiotic Lactobacillus was assessed using an in vitro 96-well plate assay. Both the stationary and exponential phases of Lactobacillus were tested on pre-formed biofilms of periodontal pathogens. Additionally, the impact of QS on biofilm degradation was evaluated by introducing QS compounds, N-(3-oxododecanoyl)-L-homoserine lactone (AHL) and D-galactose, to the pre-formed biofilms alongside probiotic Lactobacillus. Statistical significance (P < 0.05) was determined using a paired t-test with GraphPad Prism software. The results demonstrated that all probiotic Lactobacillus strains exhibited moderate inhibition activity against A. actinomycetemcomitans ATCC 29522, with the exception of L. fermentum JCM1137, which showed low inhibition activity. In contrast, strong inhibition activity was observed against P. aeruginosa ATCC 17934 by three out of five probiotic Lactobacilli, while L. fermentum JCM1137 and L. johnsonii NBRC 13952 exhibited intermediate inhibition. When exposed to pre-formed biofilms of A. actinomycetemcomitans ATCC 29522, all probiotic Lactobacilli demonstrated moderate to good biofilm degradation activity in both exponential and stationary phases, except for L. johnsonii NBRC 13952. For P. aeruginosa ATCC 17934, most probiotic Lactobacilli exhibited good to excellent biofilm degradation activity. The introduction of AHL and D-galactose resulted in significant anti-biofilm activity compared to controls, with both compounds showing greater influence at higher concentrations. In conclusion, probiotics play a significant role in degrading biofilms formed by A. actinomycetemcomitans and P. aeruginosa in both exponential and stationary growth phases. The QS influence on biofilm degradation activity was observed to be selective, with specific probiotic Lactobacillus strains responding more significantly to higher concentrations of AHL and D-galactose. These findings underscore the importance of understanding microbial molecular interactions and suggest potential strategies for improving oral health. 2025-03-24 08:58 uuid:2F0729CA-F9BE-4C8D-B57A-1D04BB457DAB 2025_Interaction Of Probiotic Lactobacillus Species With Periodontal Pathogens On Biofilm Degradation And Quorum Sensing Activity INTERACTION OF PROBIOTIC LACTOBACILLUS SPECIES WITH PERIODONTAL PATHOGENS ON BIOFILM DEGRADATION AND QUORUM SENSING ACTIVITY.pdf Thesis |
| spellingShingle | 2025_Interaction Of Probiotic Lactobacillus Species With Periodontal Pathogens On Biofilm Degradation And Quorum Sensing Activity |
| state | Terengganu |
| subject | Lactobacillus — Physiology. Dissertations-Academic Probiotics — Therapeutic use Biofilms — Degradation Quorum sensing — Inhibition |
| summary | Biofilms formed by periodontal pathogens contribute to chronic inflammation, leading to damage of connective tissues, gingival epithelium, and the alveolar bone supporting teeth. The removal of these biofilms using antibiotics is often challenging due to their limited penetration. Probiotics, such as Lactobacillus, have the potential to improve dental health by modulating the oral microbiome. Since quorum sensing (QS) plays a key role in biofilm formation, probiotic Lactobacillus may interfere with QS mechanisms, thereby influencing biofilm development. However, the underlying biochemical pathways by which probiotics affect periodontal infections remain poorly understood. This study aims to examine the biofilm formation and QS activity of Aggregatibacter actinomycetemcomitans and Pseudomonas aeruginosa, focusing on the influence of probiotic Lactobacillus strains. The antibacterial activity of probiotic Lactobacillus against A. actinomycetemcomitans and P. aeruginosa was evaluated using the spot-on-lawn method. The biofilm degradation potential of probiotic Lactobacillus was assessed using an in vitro 96-well plate assay. Both the stationary and exponential phases of Lactobacillus were tested on pre-formed biofilms of periodontal pathogens. Additionally, the impact of QS on biofilm degradation was evaluated by introducing QS compounds, N-(3-oxododecanoyl)-L-homoserine lactone (AHL) and D-galactose, to the pre-formed biofilms alongside probiotic Lactobacillus. Statistical significance (P < 0.05) was determined using a paired t-test with GraphPad Prism software. The results demonstrated that all probiotic Lactobacillus strains exhibited moderate inhibition activity against A. actinomycetemcomitans ATCC 29522, with the exception of L. fermentum JCM1137, which showed low inhibition activity. In contrast, strong inhibition activity was observed against P. aeruginosa ATCC 17934 by three out of five probiotic Lactobacilli, while L. fermentum JCM1137 and L. johnsonii NBRC 13952 exhibited intermediate inhibition. When exposed to pre-formed biofilms of A. actinomycetemcomitans ATCC 29522, all probiotic Lactobacilli demonstrated moderate to good biofilm degradation activity in both exponential and stationary phases, except for L. johnsonii NBRC 13952. For P. aeruginosa ATCC 17934, most probiotic Lactobacilli exhibited good to excellent biofilm degradation activity. The introduction of AHL and D-galactose resulted in significant anti-biofilm activity compared to controls, with both compounds showing greater influence at higher concentrations. In conclusion, probiotics play a significant role in degrading biofilms formed by A. actinomycetemcomitans and P. aeruginosa in both exponential and stationary growth phases. The QS influence on biofilm degradation activity was observed to be selective, with specific probiotic Lactobacillus strains responding more significantly to higher concentrations of AHL and D-galactose. These findings underscore the importance of understanding microbial molecular interactions and suggest potential strategies for improving oral health. |
| title | 2025_Interaction Of Probiotic Lactobacillus Species With Periodontal Pathogens On Biofilm Degradation And Quorum Sensing Activity |
| title_full | 2025_Interaction Of Probiotic Lactobacillus Species With Periodontal Pathogens On Biofilm Degradation And Quorum Sensing Activity |
| title_fullStr | 2025_Interaction Of Probiotic Lactobacillus Species With Periodontal Pathogens On Biofilm Degradation And Quorum Sensing Activity |
| title_full_unstemmed | 2025_Interaction Of Probiotic Lactobacillus Species With Periodontal Pathogens On Biofilm Degradation And Quorum Sensing Activity |
| title_short | 2025_Interaction Of Probiotic Lactobacillus Species With Periodontal Pathogens On Biofilm Degradation And Quorum Sensing Activity |
| title_sort | 2025_interaction of probiotic lactobacillus species with periodontal pathogens on biofilm degradation and quorum sensing activity |