2021_Metabolic Syndrome Among Temiar Orang Asli Community in Kuala Betis, Gua Musang, Kelantan: An Evaluation of Genetic Polymorphisms and Biochemical Analysis
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| country | Malaysia |
| date | 2021-12-15 |
| format | General Document |
| id | 15837 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | METABO~1.PDF |
| person | Nur Sakinah Binti Harun |
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| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15837 |
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| spelling | 15837 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15837 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Health Sciences English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 234 METABO~1.PDF 2021-12-15 2021_Metabolic Syndrome Among Temiar Orang Asli Community in Kuala Betis, Gua Musang, Kelantan: An Evaluation of Genetic Polymorphisms and Biochemical Analysis Nur Sakinah Binti Harun Metabolic syndrome—Genetic aspects Metabolic syndrome (MetS) is a risk factor for cardiovascular diseases. MetS is related to complex gene-environment interactions. Temiar orang asli (OA) community provides a unique opportunity to investigate gene-environment interactions since their new settlement forced them to unfavorable nature of modern living. This study aimed to determine the prevalence of MetS, other cardio-metabolic risk factors, and to investigate the association between the genetic variants of ADIPOQ rs2241766, ADIPOQ rs1501299, RETN rs1862513 and FTO rs9939609 with the risk of developing MetS among the resettled Temiar subtribe. A cross-sectional study was conducted among 123 Temiar volunteers after obtaining an informed consent. MetS was diagnosed based on modified National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) criteria. Anthropometric and biochemical measurements including serum adiponectin and resistin were performed. DNA was extracted from blood, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to genotype ADIPOQ rs2241766, ADIPOQ rs1501299, RETN rs1862513 and FTO rs9939609 polymorphisms, and were categorized into homozygous wildtype, heterozygous and homozygous variant. Statistical analysis was performed using an independent t-test for continuous variables and chi-square for categorical variables. The association between variables was determined using binary logistic regression and odds ratios (ORs). The prevalence of MetS among Temiar subtribe was 39.8%. MetS was significantly associated with gender (P=0.05), age (P=0.025), education level (P=0.004) and family history of diabetes mellitus (P=0.011). Levels of total cholesterol (P< 0.0001), potassium (P=0.019), total protein (P=0.01), alanine aminotransferase (P=0.013), adiponectin (P< 0.0001) and resistin (P=0.0002) were also significantly associated with MetS. Adiponectin was inversely correlated with BMI (r= -0.443), waist circumference (WC) (r= -0.361), systolic blood pressure (BP) (r= - 0.181), diastolic BP (r= -0.332), blood glucose level (r= -0.278), triglycerides (r= -0.402), and total cholesterol (r= -0.255), while, high density lipoprotein (HDL) (r= 0.342) was directly correlated with adiponectin. Resistin was directly correlated with BMI (r= 0.316), WC (r= 0.233), blood glucose level (r= 0.292), and total cholesterol (r= 0.216), while, HDL (r= -0.34) was inversely correlated with resistin. ADIPOQ rs2241766, ADIPOQ rs1501299, RETN rs1862513 dan FTO rs9939609 polymorphisms were significantly associated with the risk of MetS susceptibility among the Temiar subtribe, with estimated OR 87.2 (P<0.001) for heterozygous (T/G), and 26.5 (P=0.003) for homozygous (G/G) genotype for ADIPOQ rs2241766 locus; OR 38.2 (P<0.001) for heterozygous (G/T), and 19.7 (P=0.01) for homozygous (T/T) genotype at ADIPOQ rs1501299 locus; OR 222.5 (P<0.001) for heterozygous (C/G), and 26.2 (P=0.005) for homozygous (G/G) genotype at RETN rs1862513 locus; OR 19.9 (P<0.001) for heterozygous (T/A), and 20.7 (P=0.006) for homozygous (A/A) genotype at FTO rs9939609 locus. Age, BMI, blood pressure, level of serum adiponectin and serum resistin were the independent predictors of MetS among Temiar subtribe. The present study provides a significant finding suggesting a pivotal role of these polymorphisms (ADIPOQ rs2241766, ADIPOQ rs1501299, RETN rs1862513 and FTO rs9939609) in predisposing Temiar individuals to MetS. The high OR value associated with these polymorphisms could be regarded as a putative “at-risk” genetic predisposition that contribute to the susceptibility risk for MetS. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Metabolic Syndrome in Indigenous Populations Genetic Polymorphisms And Metabolic Disorders Orang Asli Health And Metabolic Risk Thesis |
| spellingShingle | 2021_Metabolic Syndrome Among Temiar Orang Asli Community in Kuala Betis, Gua Musang, Kelantan: An Evaluation of Genetic Polymorphisms and Biochemical Analysis |
| state | Terengganu |
| subject | Metabolic syndrome—Genetic aspects Dissertations, Academic |
| summary | Metabolic syndrome (MetS) is a risk factor for cardiovascular diseases. MetS is related to complex gene-environment interactions. Temiar orang asli (OA) community provides a unique opportunity to investigate gene-environment interactions since their new settlement forced them to unfavorable nature of modern living. This study aimed to determine the prevalence of MetS, other cardio-metabolic risk factors, and to investigate the association between the genetic variants of ADIPOQ rs2241766, ADIPOQ rs1501299, RETN rs1862513 and FTO rs9939609 with the risk of developing MetS among the resettled Temiar subtribe. A cross-sectional study was conducted among 123 Temiar volunteers after obtaining an informed consent. MetS was diagnosed based on modified National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) criteria. Anthropometric and biochemical measurements including serum adiponectin and resistin were performed. DNA was extracted from blood, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to genotype ADIPOQ rs2241766, ADIPOQ rs1501299, RETN rs1862513 and FTO rs9939609 polymorphisms, and were categorized into homozygous wildtype, heterozygous and homozygous variant. Statistical analysis was performed using an independent t-test for continuous variables and chi-square for categorical variables. The association between variables was determined using binary logistic regression and odds ratios (ORs). The prevalence of MetS among Temiar subtribe was 39.8%. MetS was significantly associated with gender (P=0.05), age (P=0.025), education level (P=0.004) and family history of diabetes mellitus (P=0.011). Levels of total cholesterol (P< 0.0001), potassium (P=0.019), total protein (P=0.01), alanine aminotransferase (P=0.013), adiponectin (P< 0.0001) and resistin (P=0.0002) were also significantly associated with MetS. Adiponectin was inversely correlated with BMI (r= -0.443), waist circumference (WC) (r= -0.361), systolic blood pressure (BP) (r= - 0.181), diastolic BP (r= -0.332), blood glucose level (r= -0.278), triglycerides (r= -0.402), and total cholesterol (r= -0.255), while, high density lipoprotein (HDL) (r= 0.342) was directly correlated with adiponectin. Resistin was directly correlated with BMI (r= 0.316), WC (r= 0.233), blood glucose level (r= 0.292), and total cholesterol (r= 0.216), while, HDL (r= -0.34) was inversely correlated with resistin. ADIPOQ rs2241766, ADIPOQ rs1501299, RETN rs1862513 dan FTO rs9939609 polymorphisms were significantly associated with the risk of MetS susceptibility among the Temiar subtribe, with estimated OR 87.2 (P<0.001) for heterozygous (T/G), and 26.5 (P=0.003) for homozygous (G/G) genotype for ADIPOQ rs2241766 locus; OR 38.2 (P<0.001) for heterozygous (G/T), and 19.7 (P=0.01) for homozygous (T/T) genotype at ADIPOQ rs1501299 locus; OR 222.5 (P<0.001) for heterozygous (C/G), and 26.2 (P=0.005) for homozygous (G/G) genotype at RETN rs1862513 locus; OR 19.9 (P<0.001) for heterozygous (T/A), and 20.7 (P=0.006) for homozygous (A/A) genotype at FTO rs9939609 locus. Age, BMI, blood pressure, level of serum adiponectin and serum resistin were the independent predictors of MetS among Temiar subtribe. The present study provides a significant finding suggesting a pivotal role of these polymorphisms (ADIPOQ rs2241766, ADIPOQ rs1501299, RETN rs1862513 and FTO rs9939609) in predisposing Temiar individuals to MetS. The high OR value associated with these polymorphisms could be regarded as a putative “at-risk” genetic predisposition that contribute to the susceptibility risk for MetS. |
| title | 2021_Metabolic Syndrome Among Temiar Orang Asli Community in Kuala Betis, Gua Musang, Kelantan: An Evaluation of Genetic Polymorphisms and Biochemical Analysis |
| title_full | 2021_Metabolic Syndrome Among Temiar Orang Asli Community in Kuala Betis, Gua Musang, Kelantan: An Evaluation of Genetic Polymorphisms and Biochemical Analysis |
| title_fullStr | 2021_Metabolic Syndrome Among Temiar Orang Asli Community in Kuala Betis, Gua Musang, Kelantan: An Evaluation of Genetic Polymorphisms and Biochemical Analysis |
| title_full_unstemmed | 2021_Metabolic Syndrome Among Temiar Orang Asli Community in Kuala Betis, Gua Musang, Kelantan: An Evaluation of Genetic Polymorphisms and Biochemical Analysis |
| title_short | 2021_Metabolic Syndrome Among Temiar Orang Asli Community in Kuala Betis, Gua Musang, Kelantan: An Evaluation of Genetic Polymorphisms and Biochemical Analysis |
| title_sort | 2021_metabolic syndrome among temiar orang asli community in kuala betis, gua musang, kelantan: an evaluation of genetic polymorphisms and biochemical analysis |