2022_Acute and Sub-Acute Effects of Selected Durian, Βeta-Glucan, and its Combination on Cardiovascular Risk Markers and Gut Hormones in Normal Male Sprague Dawley Rats
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| spelling | 15780 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15780 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Health Sciences English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.4.24; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 2022-00-00 188 ACUTEA~1.PDF 2022_Acute and Sub-Acute Effects of Selected Durian, Βeta-Glucan, and its Combination on Cardiovascular Risk Markers and Gut Hormones in Normal Male Sprague Dawley Rats Nur Atirah Binti A Aziz Durian—Health effects Durian (Durio zibethinus Murr.) is a popular tropical fruit in Southeast Asian countries. It is rich in macronutrients, bioactive compounds and volatile compounds. Durian may be used as a source of bioactive ingredients in combination with other food component such as β-glucan. β-Glucan was approved by European Food Safety Authority for its health benefits of lowering blood glucose and blood cholesterol. Hence, this study was aimed to determine the effects of selected durian, β-glucan, and its combination on cardiovascular risk markers (glucose, insulin, C-peptide, lipid profiles) and gut hormones (peptide YY, glucagon-like peptide-1, cholecystokinin) in healthy male Sprague Dawley rats. The studies were divided into acute (single dose - postprandially measured up to 2 h) and subacute (0 to 14 days) studies. The rats were divided into the following groups for acute study: 1) Positive Control 2) Durian 3) β-Glucan and 4) Durian + β-Glucan. There was an additional group, 5) Negative Control for sub-acute study. Frozen durian pulp containing 50 g available carbohydrate was used to prepare the formulation. The β-glucan dose was use according to EFSA recommendation, i.e. 4 g of β-glucan with 30 g of available carbohydrates to reduce glycaemic response in human. All formulations contain similar total carbohydrate and were prepared in 0.03% carboxymethyl cellulose (CMC) as vehicle. The dose was converted to animal equivalent dose (AED) using a humanequivalent dose (HED). The formulations were given using oral gavage technique. The postprandial glycaemic response was determined at baseline (0 min) to 120 min for acute study, while rats were anaesthetized using pentobarbital 200 mg/mL and blood was collected by cardiac puncture for further biochemical analysis. Durian + β-Glucan group showed significantly (p < 0.05) lower food intake compared with all groups in acute study, and lower than β-Glucan and Negative Control groups at week 2 in sub-acute study (adjusted for baseline). Durian + β-Glucan significantly (p < 0.05) reduced glucose response at 120 min compared with Positive Control in the acute study (adjusted for baseline). There were no significant differences in fasting glycaemic response, insulin and C-peptide levels compared with controls and baseline for all treatment groups in sub-acute study. Rats fed with Durian + β-Glucan showed significantly (p < 0.003) higher LDL cholesterol compared with baseline with 0.42 (0.37 to 0.54) mmol/L and 0.16 (0.08 to 0.26) mmol/L, respectively. Rats supplemented with Durian and/or β-Glucan showed similar peptide YY, glucagonlike peptide-1 and cholecystokinin. This study suggests that a combination of Durian + β-Glucan potentially reduced food intake and postprandial glycaemic response, but increased LDL-cholesterol. Lower food intake and reduced glycaemic response could be due to increase stomach distention or slower gastric emptying. Fat content in durian could increase LDL-cholesterol but this need to be confirmed in human study. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Durian And Cardiovascular Health Makers Beta-Glucan Effects On Metabolic Parameters Gut Hormone Response To Functional Foods Thesis |
| spellingShingle | 2022_Acute and Sub-Acute Effects of Selected Durian, Βeta-Glucan, and its Combination on Cardiovascular Risk Markers and Gut Hormones in Normal Male Sprague Dawley Rats |
| state | Terengganu |
| subject | Durian—Health effects Dissertations, Academic |
| summary | Durian (Durio zibethinus Murr.) is a popular tropical fruit in Southeast Asian countries. It is rich in macronutrients, bioactive compounds and volatile compounds. Durian may be used as a source of bioactive ingredients in combination with other food component such as β-glucan. β-Glucan was approved by European Food Safety Authority for its health benefits of lowering blood glucose and blood cholesterol. Hence, this study was aimed to determine the effects of selected durian, β-glucan, and its combination on cardiovascular risk markers (glucose, insulin, C-peptide, lipid profiles) and gut hormones (peptide YY, glucagon-like peptide-1, cholecystokinin) in healthy male Sprague Dawley rats. The studies were divided into acute (single dose - postprandially measured up to 2 h) and subacute (0 to 14 days) studies. The rats were divided into the following groups for acute study: 1) Positive Control 2) Durian 3) β-Glucan and 4) Durian + β-Glucan. There was an additional group, 5) Negative Control for sub-acute study. Frozen durian pulp containing 50 g available carbohydrate was used to prepare the formulation. The β-glucan dose was use according to EFSA recommendation, i.e. 4 g of β-glucan with 30 g of available carbohydrates to reduce glycaemic response in human. All formulations contain similar total carbohydrate and were prepared in 0.03% carboxymethyl cellulose (CMC) as vehicle. The dose was converted to animal equivalent dose (AED) using a humanequivalent dose (HED). The formulations were given using oral gavage technique. The postprandial glycaemic response was determined at baseline (0 min) to 120 min for acute study, while rats were anaesthetized using pentobarbital 200 mg/mL and blood was collected by cardiac puncture for further biochemical analysis. Durian + β-Glucan group showed significantly (p < 0.05) lower food intake compared with all groups in acute study, and lower than β-Glucan and Negative Control groups at week 2 in sub-acute study (adjusted for baseline). Durian + β-Glucan significantly (p < 0.05) reduced glucose response at 120 min compared with Positive Control in the acute study (adjusted for baseline). There were no significant differences in fasting glycaemic response, insulin and C-peptide levels compared with controls and baseline for all treatment groups in sub-acute study. Rats fed with Durian + β-Glucan showed significantly (p < 0.003) higher LDL cholesterol compared with baseline with 0.42 (0.37 to 0.54) mmol/L and 0.16 (0.08 to 0.26) mmol/L, respectively. Rats supplemented with Durian and/or β-Glucan showed similar peptide YY, glucagonlike peptide-1 and cholecystokinin. This study suggests that a combination of Durian + β-Glucan potentially reduced food intake and postprandial glycaemic response, but increased LDL-cholesterol. Lower food intake and reduced glycaemic response could be due to increase stomach distention or slower gastric emptying. Fat content in durian could increase LDL-cholesterol but this need to be confirmed in human study. |
| title | 2022_Acute and Sub-Acute Effects of Selected Durian, Βeta-Glucan, and its Combination on Cardiovascular Risk Markers and Gut Hormones in Normal Male Sprague Dawley Rats |
| title_full | 2022_Acute and Sub-Acute Effects of Selected Durian, Βeta-Glucan, and its Combination on Cardiovascular Risk Markers and Gut Hormones in Normal Male Sprague Dawley Rats |
| title_fullStr | 2022_Acute and Sub-Acute Effects of Selected Durian, Βeta-Glucan, and its Combination on Cardiovascular Risk Markers and Gut Hormones in Normal Male Sprague Dawley Rats |
| title_full_unstemmed | 2022_Acute and Sub-Acute Effects of Selected Durian, Βeta-Glucan, and its Combination on Cardiovascular Risk Markers and Gut Hormones in Normal Male Sprague Dawley Rats |
| title_short | 2022_Acute and Sub-Acute Effects of Selected Durian, Βeta-Glucan, and its Combination on Cardiovascular Risk Markers and Gut Hormones in Normal Male Sprague Dawley Rats |
| title_sort | 2022_acute and sub-acute effects of selected durian, βeta-glucan, and its combination on cardiovascular risk markers and gut hormones in normal male sprague dawley rats |