2023_The Effect of Short-Term Bee Bread Supplementation on the Reproductive System of Male Sprague Dawley Rats

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date 2023-07-18
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internalnotes Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih
originalfilename THE EFFECT OF SHORT-TERM BEE BREAD SUPPLEMENTATION ON THE REPRODUCTIVE SYSTEM OF MALE SPRAGUE DAWLEY RATS (MASTER_2023).pdf
person Fatimah Hamizah Binti Zakaria
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spelling 15652 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15652 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 128 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 2023-07-18 THE EFFECT OF SHORT-TERM BEE BREAD SUPPLEMENTATION ON THE REPRODUCTIVE SYSTEM OF MALE SPRAGUE DAWLEY RATS (MASTER_2023).pdf Fatimah Hamizah Binti Zakaria 2023_The Effect of Short-Term Bee Bread Supplementation on the Reproductive System of Male Sprague Dawley Rats Traditionally, people consumed bee bread for male fertility enrichment and used it mainly in folk medicine. The benefit of bee bread and its underlying potential mechanism for protecting or improving male reproductive system have not fully discovered. Hence, this study aimed to determine the effect of bee bread supplementation on male reproductive organs' weight (testis, epididymis, prostate gland and seminal vesicle), testicular cell development (seminiferous tubular diameter (STD) and seminiferous epithelial height (SEH)), sperm parameters (sperm count, sperm morphology and sperm motility) and level of sex hormones (testosterone) in Sprague Dawley rats. Male Sprague Dawley rats (n = 24; 6/group; age = 8-10 weeks) were divided into four treatment groups, Control (C: 0 g bee bread/kg body weight); Treatment 1 (T1: 1 g bee bread/kg body weight); Treatment 2 (T2: 2 g bee bread/kg body weight); and Treatment 3 (T3: 3 g bee bread/kg body weight). The rats were administered force feeding with different dilutions of bee bread in 1 ml distilled water based on their treatment groups by using a syringe, and oral gavages needle for 28 days of treatment. There were no differences (p>0.05) observed between control and treatment groups on testis weight, seminal vesicle, epididymis, prostate gland, sperm count and sperm motility. However, sperm morphology of treatment groups was lower (p<0.05) compared to control group. The level of testosterone and were higher (p<0.05) in T1, T2 and T3 groups than C group. The diameter of seminiferous tubule was higher (p<0.05) in T2 groups compared other treatment groups (C, T1 and T3). Furthermore, the seminiferous epithelial height was greater (p<0.05) in T2 group compared to C and T1 groups. In conclusion, this study suggests that supplementation of bee bread at 2 g per kg body weight has beneficial effects towards male reproductive system by reducing sperm abnormalities, increased testosterone level and improved the cellular development of testis. This effect could possibly be due to nutritional value and androgenic properties in bee bread which may function by decreasing the abnormalities of sperm morphology, increase the thickness of testicular cell development in seminiferous tubular diameter (STD) and seminiferous epithelial height (SEH), increase hormonal level of serum testosterone that could improve male fertility. Bee bread – Physiological effects Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Bee Bread Supplementation Short-Term Effects Reproductive System Thesis
spellingShingle 2023_The Effect of Short-Term Bee Bread Supplementation on the Reproductive System of Male Sprague Dawley Rats
state Terengganu
subject Bee bread – Physiological effects
Dissertations, Academic
summary Traditionally, people consumed bee bread for male fertility enrichment and used it mainly in folk medicine. The benefit of bee bread and its underlying potential mechanism for protecting or improving male reproductive system have not fully discovered. Hence, this study aimed to determine the effect of bee bread supplementation on male reproductive organs' weight (testis, epididymis, prostate gland and seminal vesicle), testicular cell development (seminiferous tubular diameter (STD) and seminiferous epithelial height (SEH)), sperm parameters (sperm count, sperm morphology and sperm motility) and level of sex hormones (testosterone) in Sprague Dawley rats. Male Sprague Dawley rats (n = 24; 6/group; age = 8-10 weeks) were divided into four treatment groups, Control (C: 0 g bee bread/kg body weight); Treatment 1 (T1: 1 g bee bread/kg body weight); Treatment 2 (T2: 2 g bee bread/kg body weight); and Treatment 3 (T3: 3 g bee bread/kg body weight). The rats were administered force feeding with different dilutions of bee bread in 1 ml distilled water based on their treatment groups by using a syringe, and oral gavages needle for 28 days of treatment. There were no differences (p>0.05) observed between control and treatment groups on testis weight, seminal vesicle, epididymis, prostate gland, sperm count and sperm motility. However, sperm morphology of treatment groups was lower (p<0.05) compared to control group. The level of testosterone and were higher (p<0.05) in T1, T2 and T3 groups than C group. The diameter of seminiferous tubule was higher (p<0.05) in T2 groups compared other treatment groups (C, T1 and T3). Furthermore, the seminiferous epithelial height was greater (p<0.05) in T2 group compared to C and T1 groups. In conclusion, this study suggests that supplementation of bee bread at 2 g per kg body weight has beneficial effects towards male reproductive system by reducing sperm abnormalities, increased testosterone level and improved the cellular development of testis. This effect could possibly be due to nutritional value and androgenic properties in bee bread which may function by decreasing the abnormalities of sperm morphology, increase the thickness of testicular cell development in seminiferous tubular diameter (STD) and seminiferous epithelial height (SEH), increase hormonal level of serum testosterone that could improve male fertility.
title 2023_The Effect of Short-Term Bee Bread Supplementation on the Reproductive System of Male Sprague Dawley Rats
title_full 2023_The Effect of Short-Term Bee Bread Supplementation on the Reproductive System of Male Sprague Dawley Rats
title_fullStr 2023_The Effect of Short-Term Bee Bread Supplementation on the Reproductive System of Male Sprague Dawley Rats
title_full_unstemmed 2023_The Effect of Short-Term Bee Bread Supplementation on the Reproductive System of Male Sprague Dawley Rats
title_short 2023_The Effect of Short-Term Bee Bread Supplementation on the Reproductive System of Male Sprague Dawley Rats
title_sort 2023_the effect of short-term bee bread supplementation on the reproductive system of male sprague dawley rats