2021_Quality Control, Anti Uterine Fibroid Potential and Chemical Profiling of Orthosiphon Stamineus Benth Extracts
| Format: | General Document |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2018-08-12 |
| format | General Document |
| id | 15642 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | QUALITY CONTROL, ANTI UTERINE FIBROID POTENTIAL AND CHEMICAL PROFILING OF ORTHOSIPHON STAMINEUS BENTH EXTRACTS (MASTER_2018).pdf |
| person | Nor Hidayah Binti Ab Halim |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15642 |
| sourcemedia | Server storage Scanned document |
| spelling | 15642 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15642 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 192 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 QUALITY CONTROL, ANTI UTERINE FIBROID POTENTIAL AND CHEMICAL PROFILING OF ORTHOSIPHON STAMINEUS BENTH EXTRACTS (MASTER_2018).pdf Nor Hidayah Binti Ab Halim 2021_Quality Control, Anti Uterine Fibroid Potential and Chemical Profiling of Orthosiphon Stamineus Benth Extracts 2018-08-12 Orthosiphon stamineus This study involves quality control including physicochemical analysis, chemical profiling and biological profiling using O. stamineus raw materials and extracts such as 50% ethanolic (OSEW) and water extract (OSW). The raw materials were evaluated by physicochemical analysis such as foreign matter, total ash, acid insoluble ash, water soluble ash, moisture content and extractive values with cold and hot methods. The results of physicochemical evaluation were compared with established literature and the results showed that the samples were within the permissible limit. For biological profiling, in vitro assay were done for both O. stamineus extracts to determine which extracts are more cytotoxic to Uterine Leiomyosarcoma Cells (SK UT-1) and Normal Fibroblast Cells (NIH/3T3). The result revealed OSW to exhibit better cytotoxic effect compared to OSEW extract and showed non- toxic towards NIH/3T3 cells. Thus, OSW extract has been chosen as a potential extract to be developed as an anti-uterine fibroid. For acute toxicity study, the limited dose was adapted where a single dose of 5000 mg/kg of OSW was given orally to five rats. OSW extract safe to be used in in vivo study as there was no abnormal changes observed including body weight and respiration rate of the rats. In in vivo study, five groups of nude mice were used which is negative control and others treated with Suramin, 100 mg/kg, 200mg/kg and 400mg/kg orally. The length and width of tumor dimensions were collected as a data of this part. The in vivo treatment with OSW extract for 21 days influenced the tumor development and results showed a significant decrease in tumor volume especially for mice that have been treated with 400mg/kg dose. Determinations of primary and secondary metabolites in OS extracts were performed to determine the composition of total polysaccharides, proteins, glycosaponins, phospholipids, phenolic, and flavonoids content. From the result, analysis of phytochemical indicates that OSW extract has the highest total glycosaponins, phenolics, and phospholipids whereas OSEW extract has the highest total proteins, polysaccharides, and flavonoids. Chemical profiling using qualitative and quantitative analytical studies performed were UV-Vis, FT-IR, HPTLC and HPLC. UV-Vis and FT-IR indicate the presence of different chemical constituents and quantity in both extracts by detecting their spectra. The presences of flavonoid and triterpenoid markers have been proved using HPTLC and HPLC except for 17 amino acids which were analyzed using HPLC only. Amino acids were found more in OSW compared to OSEW such as L-aspartic acid, L-glutamic acid, L-Histidine hydrochloride monohydrate, L-threonine, L-valine, L-methionine and L-lysine. These amino acids were found to play significant roles in the assessment of anti-uterine fibroid activity. OSEW extract contained terpenoid compound but absent in OSW extract. As a conclusion, the raw materials of O. stamineus possessed acceptable quality. OSW shows positive results for in vitro and in vivo anti-uterine fibroid studies and the chemical profile of O. stamineus extracts were obtained. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Quality Control Anti-Uterine Fibroid Potential Chemical Profiling Thesis |
| spellingShingle | 2021_Quality Control, Anti Uterine Fibroid Potential and Chemical Profiling of Orthosiphon Stamineus Benth Extracts |
| state | Terengganu |
| subject | Orthosiphon stamineus Dissertations, Academic |
| summary | This study involves quality control including physicochemical analysis, chemical profiling and biological profiling using O. stamineus raw materials and extracts such as 50% ethanolic (OSEW) and water extract (OSW). The raw materials were evaluated by physicochemical analysis such as foreign matter, total ash, acid insoluble ash, water soluble ash, moisture content and extractive values with cold and hot methods. The results of physicochemical evaluation were compared with established literature and the results showed that the samples were within the permissible limit. For biological profiling, in vitro assay were done for both O. stamineus extracts to determine which extracts are more cytotoxic to Uterine Leiomyosarcoma Cells (SK UT-1) and Normal Fibroblast Cells (NIH/3T3). The result revealed OSW to exhibit better cytotoxic effect compared to OSEW extract and showed non- toxic towards NIH/3T3 cells. Thus, OSW extract has been chosen as a potential extract to be developed as an anti-uterine fibroid. For acute toxicity study, the limited dose was adapted where a single dose of 5000 mg/kg of OSW was given orally to five rats. OSW extract safe to be used in in vivo study as there was no abnormal changes observed including body weight and respiration rate of the rats. In in vivo study, five groups of nude mice were used which is negative control and others treated with Suramin, 100 mg/kg, 200mg/kg and 400mg/kg orally. The length and width of tumor dimensions were collected as a data of this part. The in vivo treatment with OSW extract for 21 days influenced the tumor development and results showed a significant decrease in tumor volume especially for mice that have been treated with 400mg/kg dose. Determinations of primary and secondary metabolites in OS extracts were performed to determine the composition of total polysaccharides, proteins, glycosaponins, phospholipids, phenolic, and flavonoids content. From the result, analysis of phytochemical indicates that OSW extract has the highest total glycosaponins, phenolics, and phospholipids whereas OSEW extract has the highest total proteins, polysaccharides, and flavonoids. Chemical profiling using qualitative and quantitative analytical studies performed were UV-Vis, FT-IR, HPTLC and HPLC. UV-Vis and FT-IR indicate the presence of different chemical constituents and quantity in both extracts by detecting their spectra. The presences of flavonoid and triterpenoid markers have been proved using HPTLC and HPLC except for 17 amino acids which were analyzed using HPLC only. Amino acids were found more in OSW compared to OSEW such as L-aspartic acid, L-glutamic acid, L-Histidine hydrochloride monohydrate, L-threonine, L-valine, L-methionine and L-lysine. These amino acids were found to play significant roles in the assessment of anti-uterine fibroid activity. OSEW extract contained terpenoid compound but absent in OSW extract. As a conclusion, the raw materials of O. stamineus possessed acceptable quality. OSW shows positive results for in vitro and in vivo anti-uterine fibroid studies and the chemical profile of O. stamineus extracts were obtained. |
| title | 2021_Quality Control, Anti Uterine Fibroid Potential and Chemical Profiling of Orthosiphon Stamineus Benth Extracts |
| title_full | 2021_Quality Control, Anti Uterine Fibroid Potential and Chemical Profiling of Orthosiphon Stamineus Benth Extracts |
| title_fullStr | 2021_Quality Control, Anti Uterine Fibroid Potential and Chemical Profiling of Orthosiphon Stamineus Benth Extracts |
| title_full_unstemmed | 2021_Quality Control, Anti Uterine Fibroid Potential and Chemical Profiling of Orthosiphon Stamineus Benth Extracts |
| title_short | 2021_Quality Control, Anti Uterine Fibroid Potential and Chemical Profiling of Orthosiphon Stamineus Benth Extracts |
| title_sort | 2021_quality control, anti uterine fibroid potential and chemical profiling of orthosiphon stamineus benth extracts |