2021_Production of Gelatine Hydrolysates from Feet of Duck and Evalution Of Gelatine Physicochemical Properties and Bioactivities
| Format: | General Document |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
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| country | Malaysia |
| date | 2022-01-04 |
| format | General Document |
| id | 15639 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | 15639_0795ddeaf263256.pdf |
| person | Fatin Arina Binti Mohd Zain |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15639 |
| sourcemedia | Server storage Scanned document |
| spelling | 15639 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15639 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 129 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 2022-01-04 15639_0795ddeaf263256.pdf Fatin Arina Binti Mohd Zain 2021_Production of Gelatine Hydrolysates from Feet of Duck and Evalution Of Gelatine Physicochemical Properties and Bioactivities Malaysia is one of the main producers of duck meat globally with anually increasing demands. Increasing in duck production will also increase the number of duck by products such as feet skin, feet and bones. Previous study reported that the reason for choosing duck feet as a raw material for collagen and gelatin production is due to its complex part of bones and tendons. This study focused on production of gelatin from acid pretreatment and comparison of physicochemical properties between three breeds of duck, Khaki Campbell, Pekin and Muscovy with commercial gelatin (bovine). After that, the suitable enzyme (Alcalase, Esperase, Flavourzyme, Neutrase and Protamex) were used to produce duck feet gelatin hydrolysate under different enzyme-substrate ratio (1:10, 1:15 and 1:20). Finally, the bioactivities of duck feet gelatin hydrolysate such as antioxidative, antihypertensive and antiproliferative activities were determined. Three different breed of duck feet, Khaki Campbell, Pekin and Muscovy were treated with 4% acetic acid to obtain the gelatin. The physicochemical properties of gelatin of all the duck feet gelatin were compared with commercial gelatin. Pekin Duck Feet Gelatin (PDFG) has been choose to be hydrolyzed with commercial enzymes (Alcalase, Esperase, Flavourzyme, Neutrase and Protamex) to produce Pekin Duck Feet Gelatin Hydrolysate (PDFGH) because it produces the highest yield value. All the enzymes were analyzed with different enzyme-substrate ratio (1:10, 1:15, 1:20) under optimum pH and temperature. Antihypertensive activity, scavenging activities and antiproliferative activity on breast cancer (MCF-7) cell line were determined. From this study it was found that the physicochemical properties of duck feet gelatin (DFG) produce different colour and functional properties. Among all the three breeds, PDFG has the highest yield value with average value of 1.91% followed by KCDFG (1.65%) and lastly MDFG (1.40%). This quality has made PDFG as a potential raw material that can be used to produce bioactive peptide. The highest yield of Pekin Duck Feet Gelatin Hydrolysate (PDFGH) with average value of 75.33% and 80.02% has been produced by using Protamex (1:20) and Neutrase (1:15) respectively. Alcalase (1:10) and Neutrase (1:10) have produced DFGH with the highest inhibition value which is 1.71% and 1.54% respectively for ACE inhibition activity. Meanwhile, PDFGH that was hydrolyzed by using Protamex (1:20) and Alcalase (1:20) have the highest DPPH scavenging activity which is 54.83% and 53.12% respectively. Esperase (1:20) and Protamex (1:10) had shown the lowest IC50 value against breast cancer (MCF-7) cell line with the average value of 26.40 mg/ml and 27.96 mg/ml PDFG produced a higher production yield (1.91%) compare to KCDFG (1.65%) and MDFG (1.40%). The PDFG also produced high yield content and different biofunctional properties after being hydrolyzed with enzyme. Duck feet gelatin is a potential raw material that can be used to produce bioactive peptide hence reducing the waste product. Gelatine – Production Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Physicochemical Properties Duck Feet Gelatine Hydrolysates Thesis |
| spellingShingle | 2021_Production of Gelatine Hydrolysates from Feet of Duck and Evalution Of Gelatine Physicochemical Properties and Bioactivities |
| state | Terengganu |
| subject | Gelatine – Production Dissertations, Academic |
| summary | Malaysia is one of the main producers of duck meat globally with anually increasing demands. Increasing in duck production will also increase the number of duck by products such as feet skin, feet and bones. Previous study reported that the reason for choosing duck feet as a raw material for collagen and gelatin production is due to its complex part of bones and tendons. This study focused on production of gelatin from acid pretreatment and comparison of physicochemical properties between three breeds of duck, Khaki Campbell, Pekin and Muscovy with commercial gelatin (bovine). After that, the suitable enzyme (Alcalase, Esperase, Flavourzyme, Neutrase and Protamex) were used to produce duck feet gelatin hydrolysate under different enzyme-substrate ratio (1:10, 1:15 and 1:20). Finally, the bioactivities of duck feet gelatin hydrolysate such as antioxidative, antihypertensive and antiproliferative activities were determined. Three different breed of duck feet, Khaki Campbell, Pekin and Muscovy were treated with 4% acetic acid to obtain the gelatin. The physicochemical properties of gelatin of all the duck feet gelatin were compared with commercial gelatin. Pekin Duck Feet Gelatin (PDFG) has been choose to be hydrolyzed with commercial enzymes (Alcalase, Esperase, Flavourzyme, Neutrase and Protamex) to produce Pekin Duck Feet Gelatin Hydrolysate (PDFGH) because it produces the highest yield value. All the enzymes were analyzed with different enzyme-substrate ratio (1:10, 1:15, 1:20) under optimum pH and temperature. Antihypertensive activity, scavenging activities and antiproliferative activity on breast cancer (MCF-7) cell line were determined. From this study it was found that the physicochemical properties of duck feet gelatin (DFG) produce different colour and functional properties. Among all the three breeds, PDFG has the highest yield value with average value of 1.91% followed by KCDFG (1.65%) and lastly MDFG (1.40%). This quality has made PDFG as a potential raw material that can be used to produce bioactive peptide. The highest yield of Pekin Duck Feet Gelatin Hydrolysate (PDFGH) with average value of 75.33% and 80.02% has been produced by using Protamex (1:20) and Neutrase (1:15) respectively. Alcalase (1:10) and Neutrase (1:10) have produced DFGH with the highest inhibition value which is 1.71% and 1.54% respectively for ACE inhibition activity. Meanwhile, PDFGH that was hydrolyzed by using Protamex (1:20) and Alcalase (1:20) have the highest DPPH scavenging activity which is 54.83% and 53.12% respectively. Esperase (1:20) and Protamex (1:10) had shown the lowest IC50 value against breast cancer (MCF-7) cell line with the average value of 26.40 mg/ml and 27.96 mg/ml PDFG produced a higher production yield (1.91%) compare to KCDFG (1.65%) and MDFG (1.40%). The PDFG also produced high yield content and different biofunctional properties after being hydrolyzed with enzyme. Duck feet gelatin is a potential raw material that can be used to produce bioactive peptide hence reducing the waste product. |
| title | 2021_Production of Gelatine Hydrolysates from Feet of Duck and Evalution Of Gelatine Physicochemical Properties and Bioactivities |
| title_full | 2021_Production of Gelatine Hydrolysates from Feet of Duck and Evalution Of Gelatine Physicochemical Properties and Bioactivities |
| title_fullStr | 2021_Production of Gelatine Hydrolysates from Feet of Duck and Evalution Of Gelatine Physicochemical Properties and Bioactivities |
| title_full_unstemmed | 2021_Production of Gelatine Hydrolysates from Feet of Duck and Evalution Of Gelatine Physicochemical Properties and Bioactivities |
| title_short | 2021_Production of Gelatine Hydrolysates from Feet of Duck and Evalution Of Gelatine Physicochemical Properties and Bioactivities |
| title_sort | 2021_production of gelatine hydrolysates from feet of duck and evalution of gelatine physicochemical properties and bioactivities |