2021_Prevalence, Antibiogram And Biofilm Formation of Listeria monocytogenes In Chicken
| Format: | General Document |
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| building | INTELEK Repository |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2021-11-16 |
| format | General Document |
| id | 15637 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | PREVALENCE, ANTIBIOGRAM AND BIOFILM FORMATION OF Listeria monocytogenes IN CHICKEN (MASTER_2021).pdf |
| person | Norhadirah Binti Mohd Noor |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15637 |
| sourcemedia | Server storage Scanned document |
| spelling | 15637 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15637 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Listeria monocytogenes Copyright©PWB2025 2021-11-16 137 PREVALENCE, ANTIBIOGRAM AND BIOFILM FORMATION OF Listeria monocytogenes IN CHICKEN (MASTER_2021).pdf Norhadirah Binti Mohd Noor 2021_Prevalence, Antibiogram And Biofilm Formation of Listeria monocytogenes In Chicken Listeria monocytogenes is a psychrotrophic, Gram positive bacteria which can cause infection among high- risk group such as infant, elderly and immunocompromised patient. This study aimed to determine the prevalence of L. monocytogenes in chickens from wet market, to characterize L. monocytogenes isolates using molecular method and antibiotic sensitivity test, to investigate the survival patterns and biofilm formation capability of L. monocytogenes and to evaluate the efficiency of different household chemicals against L. monocytogenes. Real time Polymerase Chain Reaction (PCR) assay and conventional plating method were used to detect and isolate L. monocytogenes in 48 chicken samples from wet markets located in three Terengganu districts (Besut, Kuala Terengganu and Setiu). The L. monocytogenes isolates were tested for antimicrobial susceptibility using disc diffusion method against 14 types of antibiotics. Random Amplification Polymorphic DNA (RAPD) PCR assay was performed to characterize six L. monocytogenes isolates. For survivability study and biofilm formation, L. monocytogenes was inoculated on three types of food contact surfaces (stainless steel, glass and plastic) and enumeration was done after specified incubation time. For biofilm formation of L. monocytogenes was performed using microtiter plate assay. Three different types of nutrient medium (tryptic soy broth (TSB), chicken wash and chicken broth) were assessed on their effects on L. monocytogenes biofilm formation. Lastly, six different types of chemicals (bleach, acetic acid, baking soda, dish detergent, floor detergent and antiseptic) were done using disc diffusion assay to screen the antibacterial activity against L. monocytogenes. Chemicals with highest antibacterial activity were evaluated for their Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) using macro- dilution broth method. The prevalence of L. monocytogenes detected by real time PCR and plating methods were 16.7% and 12.5%, respectively. L. monocytogenes isolates showed the highest resistance towards vancomycin (100%) and lowest resistance against enrofloxacin and amikacin (12.5%). All L. monocytogenes isolates showed multiple antibiotics resistance. RAPD clustering analysis showed that the L. monocytogenes isolates from chickens from wet markets were not correlated to each other. L. monocytogenes was found to survive on all food contact surfaces after 6 h incubation with the most recovered bacterial cell was from stainless steel followed by glass and plastic. L. monocytogenes biofilm formed the most in TSB followed by chicken broth and chicken wash. Protein analysis shown TSB has the highest concentration followed by chicken broth and chicken wash. Thus, biofilm formation of L. monocytogenes was influenced by the nutrient concentration. Two out of six chemicals tested on L. monocytogenes which are bleach and baking soda were ineffective in all solvents. MIC and MBC studies showed that dish washing detergent is the most efficient chemical to kill L. monocytogenes. This study proved that L. monocytogenes can contaminate chicken meat and food contact surfaces. Biofilm formation capability increase the risk of cross contamination of L. monocytogenes in domestic kitchen. However, dish washing detergent was found to be effective against L. monocytogenes. Thus, proper cleaning of kitchen utensils will reduce the risk of L. monocytogenes contamination. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Biofilm Formation Listeria Monocytogenes Antibiotic Resistance Thesis |
| spellingShingle | 2021_Prevalence, Antibiogram And Biofilm Formation of Listeria monocytogenes In Chicken |
| state | Terengganu |
| subject | Listeria monocytogenes Dissertations, Academic |
| summary | Listeria monocytogenes is a psychrotrophic, Gram positive bacteria which can cause infection among high- risk group such as infant, elderly and immunocompromised patient. This study aimed to determine the prevalence of L. monocytogenes in chickens from wet market, to characterize L. monocytogenes isolates using molecular method and antibiotic sensitivity test, to investigate the survival patterns and biofilm formation capability of L. monocytogenes and to evaluate the efficiency of different household chemicals against L. monocytogenes. Real time Polymerase Chain Reaction (PCR) assay and conventional plating method were used to detect and isolate L. monocytogenes in 48 chicken samples from wet markets located in three Terengganu districts (Besut, Kuala Terengganu and Setiu). The L. monocytogenes isolates were tested for antimicrobial susceptibility using disc diffusion method against 14 types of antibiotics. Random Amplification Polymorphic DNA (RAPD) PCR assay was performed to characterize six L. monocytogenes isolates. For survivability study and biofilm formation, L. monocytogenes was inoculated on three types of food contact surfaces (stainless steel, glass and plastic) and enumeration was done after specified incubation time. For biofilm formation of L. monocytogenes was performed using microtiter plate assay. Three different types of nutrient medium (tryptic soy broth (TSB), chicken wash and chicken broth) were assessed on their effects on L. monocytogenes biofilm formation. Lastly, six different types of chemicals (bleach, acetic acid, baking soda, dish detergent, floor detergent and antiseptic) were done using disc diffusion assay to screen the antibacterial activity against L. monocytogenes. Chemicals with highest antibacterial activity were evaluated for their Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) using macro- dilution broth method. The prevalence of L. monocytogenes detected by real time PCR and plating methods were 16.7% and 12.5%, respectively. L. monocytogenes isolates showed the highest resistance towards vancomycin (100%) and lowest resistance against enrofloxacin and amikacin (12.5%). All L. monocytogenes isolates showed multiple antibiotics resistance. RAPD clustering analysis showed that the L. monocytogenes isolates from chickens from wet markets were not correlated to each other. L. monocytogenes was found to survive on all food contact surfaces after 6 h incubation with the most recovered bacterial cell was from stainless steel followed by glass and plastic. L. monocytogenes biofilm formed the most in TSB followed by chicken broth and chicken wash. Protein analysis shown TSB has the highest concentration followed by chicken broth and chicken wash. Thus, biofilm formation of L. monocytogenes was influenced by the nutrient concentration. Two out of six chemicals tested on L. monocytogenes which are bleach and baking soda were ineffective in all solvents. MIC and MBC studies showed that dish washing detergent is the most efficient chemical to kill L. monocytogenes. This study proved that L. monocytogenes can contaminate chicken meat and food contact surfaces. Biofilm formation capability increase the risk of cross contamination of L. monocytogenes in domestic kitchen. However, dish washing detergent was found to be effective against L. monocytogenes. Thus, proper cleaning of kitchen utensils will reduce the risk of L. monocytogenes contamination. |
| title | 2021_Prevalence, Antibiogram And Biofilm Formation of Listeria monocytogenes In Chicken |
| title_full | 2021_Prevalence, Antibiogram And Biofilm Formation of Listeria monocytogenes In Chicken |
| title_fullStr | 2021_Prevalence, Antibiogram And Biofilm Formation of Listeria monocytogenes In Chicken |
| title_full_unstemmed | 2021_Prevalence, Antibiogram And Biofilm Formation of Listeria monocytogenes In Chicken |
| title_short | 2021_Prevalence, Antibiogram And Biofilm Formation of Listeria monocytogenes In Chicken |
| title_sort | 2021_prevalence, antibiogram and biofilm formation of listeria monocytogenes in chicken |