2023_Potential of Paenibacillus alvei And Bacillus toyonensis As Biocontrol Agents and Plant Growth Promoters for Cucumis melo var inodorus cv Manis Terengganu 1 (Melon Manis Terengganu)
| Format: | General Document |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
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| country | Malaysia |
| date | 2023-05-21 |
| format | General Document |
| id | 15635 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | 15635_29f5ec47bb6aafd.pdf |
| person | Mariana Binti Mohammad |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15635 |
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| spelling | 15635 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15635 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 134 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.4.24; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 2023-05-21 15635_29f5ec47bb6aafd.pdf Mariana Binti Mohammad 2023_Potential of Paenibacillus alvei And Bacillus toyonensis As Biocontrol Agents and Plant Growth Promoters for Cucumis melo var inodorus cv Manis Terengganu 1 (Melon Manis Terengganu) Melon Manis Terengganu (MMT) or its scientific name is Cucumis melo var inodorus cv. Manis Terengganu 1 is a new melon variety grown exclusively in Terengganu and is also known Terengganu’s iconic fruit. MMT production has been insufficient to meet local demand due to plant diseases and pests that are mainly controlled with chemical fungicides. The excessive use of chemical pesticides and fungicides to control plant diseases and pests has reduced soil fertility, impaired soil resilience, and negatively impacted the environment. Therefore, this study aims to characterize Bacillus toyonensis and Peanibacillus alvei and determine their potential as biocontrol agents and plant growth promoters on MMT. Phenotypic characterization of B. toyonensis and P. alvei were identified and characterized using morphological and molecular approaches. Pathogenic MMT fungi were isolated, and the severity of damage to MMT leaves by pathogenic fungi was analyzed using Koch's postulates. Potential B. toyonensis and P. alvei as biological control agents for MMT were also identified by dual culture tests and greenhouse experiments. Later, the efficacy of the two bacteria in promoting plant growth in the greenhouse were evaluated by treating MMT trees and soil. The beneficial bacteria were identified as B. toyonensis and P. alvei and characterized as Gram-positive and endospore-forming bacteria. Subsequently, three pathogenic fungi were successfully isolated and identified: Blumeria graminis, Penicillium limosum, and Colletotrichum dracaenophilum. Pathogenicity was confirmed by establishing Koch's postulates, which showed 100% disease infection on MMT. Moreover, dual culture tests showed the highest percentage of inhibition with P. alvei and B. toyonensis againts P. limosum by 87.7% and 89.4%, respectively. Therefore, P. limosum was selected for the greenhouse experiment, and the results showed that P. alvei, B. toyonensis, and the consortium were significantly different from the control. Thus, all tested bacteria have the potential to be used as biological control agents to control P. limosum, with B. toyonensis being the best treatment. P. alvei and B. toyonensis have the potential to improve plant growth by showing significant increments in tree height, leaves number, root mass, and root length compared to the control. While, soils treated with P. alvei and B. toyonensis also have higher concentrations of nitrate (NO3), phosphorus (P), potassium (K+), magnesium (Mg2+), calcium (Ca2+), and sulphur (S2-) compared to the control. In conclusion, these beneficial bacteria were characterized and identified as P. alvei and B. toyonensis. The results of this study suggest that both bacteria have great potential as biocontrol agents to control P. limosum and promote plant growth. These results contribute to alternative studies in microbiology and its role towards sustainable agricultural industry. Paenibacillus alvei Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Biocontrol Agents Plant Growth Promotion Paenibacillus Alvei Thesis |
| spellingShingle | 2023_Potential of Paenibacillus alvei And Bacillus toyonensis As Biocontrol Agents and Plant Growth Promoters for Cucumis melo var inodorus cv Manis Terengganu 1 (Melon Manis Terengganu) |
| state | Terengganu |
| subject | Paenibacillus alvei Dissertations, Academic |
| summary | Melon Manis Terengganu (MMT) or its scientific name is Cucumis melo var inodorus cv. Manis Terengganu 1 is a new melon variety grown exclusively in Terengganu and is also known Terengganu’s iconic fruit. MMT production has been insufficient to meet local demand due to plant diseases and pests that are mainly controlled with chemical fungicides. The excessive use of chemical pesticides and fungicides to control plant diseases and pests has reduced soil fertility, impaired soil resilience, and negatively impacted the environment. Therefore, this study aims to characterize Bacillus toyonensis and Peanibacillus alvei and determine their potential as biocontrol agents and plant growth promoters on MMT. Phenotypic characterization of B. toyonensis and P. alvei were identified and characterized using morphological and molecular approaches. Pathogenic MMT fungi were isolated, and the severity of damage to MMT leaves by pathogenic fungi was analyzed using Koch's postulates. Potential B. toyonensis and P. alvei as biological control agents for MMT were also identified by dual culture tests and greenhouse experiments. Later, the efficacy of the two bacteria in promoting plant growth in the greenhouse were evaluated by treating MMT trees and soil. The beneficial bacteria were identified as B. toyonensis and P. alvei and characterized as Gram-positive and endospore-forming bacteria. Subsequently, three pathogenic fungi were successfully isolated and identified: Blumeria graminis, Penicillium limosum, and Colletotrichum dracaenophilum. Pathogenicity was confirmed by establishing Koch's postulates, which showed 100% disease infection on MMT. Moreover, dual culture tests showed the highest percentage of inhibition with P. alvei and B. toyonensis againts P. limosum by 87.7% and 89.4%, respectively. Therefore, P. limosum was selected for the greenhouse experiment, and the results showed that P. alvei, B. toyonensis, and the consortium were significantly different from the control. Thus, all tested bacteria have the potential to be used as biological control agents to control P. limosum, with B. toyonensis being the best treatment. P. alvei and B. toyonensis have the potential to improve plant growth by showing significant increments in tree height, leaves number, root mass, and root length compared to the control. While, soils treated with P. alvei and B. toyonensis also have higher concentrations of nitrate (NO3), phosphorus (P), potassium (K+), magnesium (Mg2+), calcium (Ca2+), and sulphur (S2-) compared to the control. In conclusion, these beneficial bacteria were characterized and identified as P. alvei and B. toyonensis. The results of this study suggest that both bacteria have great potential as biocontrol agents to control P. limosum and promote plant growth. These results contribute to alternative studies in microbiology and its role towards sustainable agricultural industry. |
| title | 2023_Potential of Paenibacillus alvei And Bacillus toyonensis As Biocontrol Agents and Plant Growth Promoters for Cucumis melo var inodorus cv Manis Terengganu 1 (Melon Manis Terengganu) |
| title_full | 2023_Potential of Paenibacillus alvei And Bacillus toyonensis As Biocontrol Agents and Plant Growth Promoters for Cucumis melo var inodorus cv Manis Terengganu 1 (Melon Manis Terengganu) |
| title_fullStr | 2023_Potential of Paenibacillus alvei And Bacillus toyonensis As Biocontrol Agents and Plant Growth Promoters for Cucumis melo var inodorus cv Manis Terengganu 1 (Melon Manis Terengganu) |
| title_full_unstemmed | 2023_Potential of Paenibacillus alvei And Bacillus toyonensis As Biocontrol Agents and Plant Growth Promoters for Cucumis melo var inodorus cv Manis Terengganu 1 (Melon Manis Terengganu) |
| title_short | 2023_Potential of Paenibacillus alvei And Bacillus toyonensis As Biocontrol Agents and Plant Growth Promoters for Cucumis melo var inodorus cv Manis Terengganu 1 (Melon Manis Terengganu) |
| title_sort | 2023_potential of paenibacillus alvei and bacillus toyonensis as biocontrol agents and plant growth promoters for cucumis melo var inodorus cv manis terengganu 1 (melon manis terengganu) |