2015_Population Divergence of Swamp Eel (Monopterus albus) In East Coast Of Peninsular Malaysia Using Mitochondrial DNA Analysis
| Format: | General Document |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2016-01-11 |
| format | General Document |
| id | 15634 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | POPULATION DIVERGENCE OF SWAMP EEL (Monopterus albus) IN EAST COAST OF PENINSULAR MALAYSIA USING MITOCHONDRIAL DNA ANALYSIS.pdf |
| person | Aliyu Garba Khaleel |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15634 |
| sourcemedia | Server storage Scanned document |
| spelling | 15634 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15634 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 99 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Aliyu Garba Khaleel Copyright©PWB2025 2016-01-11 POPULATION DIVERGENCE OF SWAMP EEL (Monopterus albus) IN EAST COAST OF PENINSULAR MALAYSIA USING MITOCHONDRIAL DNA ANALYSIS.pdf 2015_Population Divergence of Swamp Eel (Monopterus albus) In East Coast Of Peninsular Malaysia Using Mitochondrial DNA Analysis The swamp eel, Monopterus albus is widely distributed in East and Southeast Asia. It can be found in rice fields, ponds and rivers. It is a highly commercial food fish, and is also used as traditional medicine especially in China and Malaysia. In Malaysia, the wild stock of swamp eel is decreasing due to over fishing. Therefore, development of mass seed production technology and aquaculture of swamp eel is highly needed, not only to increase the yield for satisfying the high demand of consumers, but also to conserve the wild swamp eel resource and increasing revenue generation. Broodstock selection is one of the important aspects in broodstock management, which is also an essential step in seed production for aquaculture. Population genetics study which is derived from the mitochondrial DNA analysis is a useful tool to identify the population structure of swamp eel for broodstock selection. However, there is insufficient data for swamp eel population structure in Malaysia. East Coast of Peninsular Malaysia (ECPM) is the second largest producing region of swamp eel in Malaysia, with many paddy fields that favoured people in the region to engage in farming of this fish species. The present study aimed to reveal the population structure of swamp eel in ECPM. A total of sixty-one samples were collected from three states (Kelantan, Terengganu and Pahang) from six different sampling sites. After DNA extraction and PCR of 16S rRNA of mtDNA using universal primer L1567 and H2196, successful sequencing (624 bp) were aligned and analyzed. Four haplotypes were detected among all the samples. Hap-1 was the most widespread haplotype among the six sampling sites, comprising about 75.4% of all samples. The haplotypes relationship as observed in phylogenetic analysis constructed by neighbor-joining tree using Kimura-2 parameter distance, 1,000 bootstrap replication with Synbranchus marmoratus as an outgroup; revealed that all four haplotypes clustered into one lineage with Hap-1 more closely related to the ancestors. Both the non-significant values of Tajima’s D and Fu’s Fs suggested that all populations were at genetic equilibrium. Analysis of Molecular Variance (AMOVA) revealed a significant differences among sampling sites (FST = 0.92401, P<0.05). In pairwise comparisons of FST, the Kuala Terengganu population showed significant values between all populations. This study suggested that it is genetically different from other populations. Non significant FST values of Pasir Puteh, Tanah Merah, Rantau Panjang, Kampung Raja and Pekan populations revealed that they are closely related populations. Further study using larger sample sizes and longer mtDNA fragment is recommended to reveal more genetic variation of these populations. Conservation initiatives on Kuala Terengganu population should be carried out to prevent the loss of these natural gene resources for future selection and cross-breeding program. Distinct haplotypes could be used as DNA markers for their respective sampling location. Swamp eel (Monopterus albus) Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Monopterus Albus Population Divergence Swamp Eel Thesis |
| spellingShingle | 2015_Population Divergence of Swamp Eel (Monopterus albus) In East Coast Of Peninsular Malaysia Using Mitochondrial DNA Analysis |
| state | Terengganu |
| subject | Swamp eel (Monopterus albus) Dissertations, Academic |
| summary | The swamp eel, Monopterus albus is widely distributed in East and Southeast Asia. It can be found in rice fields, ponds and rivers. It is a highly commercial food fish, and is also used as traditional medicine especially in China and Malaysia. In Malaysia, the wild stock of swamp eel is decreasing due to over fishing. Therefore, development of mass seed production technology and aquaculture of swamp eel is highly needed, not only to increase the yield for satisfying the high demand of consumers, but also to conserve the wild swamp eel resource and increasing revenue generation. Broodstock selection is one of the important aspects in broodstock management, which is also an essential step in seed production for aquaculture. Population genetics study which is derived from the mitochondrial DNA analysis is a useful tool to identify the population structure of swamp eel for broodstock selection. However, there is insufficient data for swamp eel population structure in Malaysia. East Coast of Peninsular Malaysia (ECPM) is the second largest producing region of swamp eel in Malaysia, with many paddy fields that favoured people in the region to engage in farming of this fish species. The present study aimed to reveal the population structure of swamp eel in ECPM. A total of sixty-one samples were collected from three states (Kelantan, Terengganu and Pahang) from six different sampling sites. After DNA extraction and PCR of 16S rRNA of mtDNA using universal primer L1567 and H2196, successful sequencing (624 bp) were aligned and analyzed. Four haplotypes were detected among all the samples. Hap-1 was the most widespread haplotype among the six sampling sites, comprising about 75.4% of all samples. The haplotypes relationship as observed in phylogenetic analysis constructed by neighbor-joining tree using Kimura-2 parameter distance, 1,000 bootstrap replication with Synbranchus marmoratus as an outgroup; revealed that all four haplotypes clustered into one lineage with Hap-1 more closely related to the ancestors. Both the non-significant values of Tajima’s D and Fu’s Fs suggested that all populations were at genetic equilibrium. Analysis of Molecular Variance (AMOVA) revealed a significant differences among sampling sites (FST = 0.92401, P<0.05). In pairwise comparisons of FST, the Kuala Terengganu population showed significant values between all populations. This study suggested that it is genetically different from other populations. Non significant FST values of Pasir Puteh, Tanah Merah, Rantau Panjang, Kampung Raja and Pekan populations revealed that they are closely related populations. Further study using larger sample sizes and longer mtDNA fragment is recommended to reveal more genetic variation of these populations. Conservation initiatives on Kuala Terengganu population should be carried out to prevent the loss of these natural gene resources for future selection and cross-breeding program. Distinct haplotypes could be used as DNA markers for their respective sampling location. |
| title | 2015_Population Divergence of Swamp Eel (Monopterus albus) In East Coast Of Peninsular Malaysia Using Mitochondrial DNA Analysis |
| title_full | 2015_Population Divergence of Swamp Eel (Monopterus albus) In East Coast Of Peninsular Malaysia Using Mitochondrial DNA Analysis |
| title_fullStr | 2015_Population Divergence of Swamp Eel (Monopterus albus) In East Coast Of Peninsular Malaysia Using Mitochondrial DNA Analysis |
| title_full_unstemmed | 2015_Population Divergence of Swamp Eel (Monopterus albus) In East Coast Of Peninsular Malaysia Using Mitochondrial DNA Analysis |
| title_short | 2015_Population Divergence of Swamp Eel (Monopterus albus) In East Coast Of Peninsular Malaysia Using Mitochondrial DNA Analysis |
| title_sort | 2015_population divergence of swamp eel (monopterus albus) in east coast of peninsular malaysia using mitochondrial dna analysis |