2018_Phytochemical analysis and Antifibroid Potential of Labisia pumila var alata Extracts
| Format: | General Document |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2018-08-12 |
| format | General Document |
| id | 15631 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | PHYTOCHEMICAL ANALYSIS AND ANTIFIBROID POTENTIAL OF LABISIA PUMILA VAR ALATA EXTRACTS (MASTER_2018).pdf |
| person | Norfahana Binti Zakaria |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15631 |
| sourcemedia | Server storage Scanned document |
| spelling | 15631 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15631 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 157 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 PHYTOCHEMICAL ANALYSIS AND ANTIFIBROID POTENTIAL OF LABISIA PUMILA VAR ALATA EXTRACTS (MASTER_2018).pdf 2018_Phytochemical analysis and Antifibroid Potential of Labisia pumila var alata Extracts Norfahana Binti Zakaria 2018-08-12 Labisia pumila var. alata (Primulacea) locally known as „Kacip Fatimahis widely used as traditional medicine in Malaysia. Presence of various phytochemicals in this plant contributed to various therapeutic activities. Currently, uterine fibroids affect a large population of woman worldwide and the available treatments options bring undesirable effect on the quality of life. Therefore, this study was conducted to investigate the anti-uterine fibroid potential of L. pumila extracts on uterine leiomyosarcoma SK-UT-1 cells. Primary and secondary metabolites analysis was conducted using standard qualitative methods. Phytochemical screening was conducted using HPTLC (High Performance Thin Layer Chromatography) analysis and further quantified by HPLC (High performance Liquid Chromatography) using gallic acid (GA) and caffeic acid (CA) as chemical markers. Cytotoxicity activity was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay using epigallocatechin gallate (EGCG) as a positive control. Morphological screening of apoptosis was monitored using phase light microscope and Acridine Orange/Propidium iodide (AO/PI) dual staining along with Annexin V-FITC/PI staining assay. Cell cycle progression was further analyzed using flow cytometer. Results revealed that ethanol-water extract (LPEW) contained highest total phenolics, flavonoids, phospholipids, and glycosaponins whereas water extract (LPW) contained the highest total proteins and polysaccharides. Furthermore, their biomarkers, gallic acid (GA) content was observed higher as compared to caffeic acid (CA) in both extracts and LPEW was dominant compared to LPW. LPEW exhibited moderate cytotoxic activity compared to LPW with IC50 of 22 µg/mL and 28 µg/mL, respectively. The bioactive marker compounds; GA and CA exhibited higher activity compared to the crude extracts with IC50 4 µg/mL and 8 µg/mL. Besides, the morphological assessment showed some changes that indicated the induction of apoptosis occurred in SK-UT-1 cells. Flow cytometry analysis showed that LPEW, LPW, GA and CA were able to arrest SK-UT-1 cells at sub G1, G0/G1 and G2/M phase. This finding suggested that L. pumila extracts and their biomarker compounds exhibited moderate cytotoxicity activity towards SK-UT-1 cells as well as apoptosis activity in a dose-dependent manner by arresting the cycle at sub G0/G1 phase. As a conclusion, L. pumila extracts and their biomarkers have the potential to be developed as an anti-fibroid agent. Labisia pumila Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Phytochemical Analysis Antifibroid Properties Labisia Pumila Var. Alata Thesis |
| spellingShingle | 2018_Phytochemical analysis and Antifibroid Potential of Labisia pumila var alata Extracts |
| state | Terengganu |
| subject | Labisia pumila Dissertations, Academic |
| summary | Labisia pumila var. alata (Primulacea) locally known as „Kacip Fatimahis widely used as traditional medicine in Malaysia. Presence of various phytochemicals in this plant contributed to various therapeutic activities. Currently, uterine fibroids affect a large population of woman worldwide and the available treatments options bring undesirable effect on the quality of life. Therefore, this study was conducted to investigate the anti-uterine fibroid potential of L. pumila extracts on uterine leiomyosarcoma SK-UT-1 cells. Primary and secondary metabolites analysis was conducted using standard qualitative methods. Phytochemical screening was conducted using HPTLC (High Performance Thin Layer Chromatography) analysis and further quantified by HPLC (High performance Liquid Chromatography) using gallic acid (GA) and caffeic acid (CA) as chemical markers. Cytotoxicity activity was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay using epigallocatechin gallate (EGCG) as a positive control. Morphological screening of apoptosis was monitored using phase light microscope and Acridine Orange/Propidium iodide (AO/PI) dual staining along with Annexin V-FITC/PI staining assay. Cell cycle progression was further analyzed using flow cytometer. Results revealed that ethanol-water extract (LPEW) contained highest total phenolics, flavonoids, phospholipids, and glycosaponins whereas water extract (LPW) contained the highest total proteins and polysaccharides. Furthermore, their biomarkers, gallic acid (GA) content was observed higher as compared to caffeic acid (CA) in both extracts and LPEW was dominant compared to LPW. LPEW exhibited moderate cytotoxic activity compared to LPW with IC50 of 22 µg/mL and 28 µg/mL, respectively. The bioactive marker compounds; GA and CA exhibited higher activity compared to the crude extracts with IC50 4 µg/mL and 8 µg/mL. Besides, the morphological assessment showed some changes that indicated the induction of apoptosis occurred in SK-UT-1 cells. Flow cytometry analysis showed that LPEW, LPW, GA and CA were able to arrest SK-UT-1 cells at sub G1, G0/G1 and G2/M phase. This finding suggested that L. pumila extracts and their biomarker compounds exhibited moderate cytotoxicity activity towards SK-UT-1 cells as well as apoptosis activity in a dose-dependent manner by arresting the cycle at sub G0/G1 phase. As a conclusion, L. pumila extracts and their biomarkers have the potential to be developed as an anti-fibroid agent. |
| title | 2018_Phytochemical analysis and Antifibroid Potential of Labisia pumila var alata Extracts |
| title_full | 2018_Phytochemical analysis and Antifibroid Potential of Labisia pumila var alata Extracts |
| title_fullStr | 2018_Phytochemical analysis and Antifibroid Potential of Labisia pumila var alata Extracts |
| title_full_unstemmed | 2018_Phytochemical analysis and Antifibroid Potential of Labisia pumila var alata Extracts |
| title_short | 2018_Phytochemical analysis and Antifibroid Potential of Labisia pumila var alata Extracts |
| title_sort | 2018_phytochemical analysis and antifibroid potential of labisia pumila var alata extracts |