2020_Molecular Prevalence and Acute Phase Protein Response of Staphylococcus Aureus Subclinical Mastitis in Goat
| Format: | General Document |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2020-12-23 |
| format | General Document |
| id | 15618 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | MOLECULAR PREVALENCE AND ACUTE PHASE PROTEIN RESPONSE OF Staphylococcus aureus SUBCLINICAL MASTITIS IN GOAT (MASTER_2020).pdf |
| person | Muhammad Faiq Bin Tajol Ariffin |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15618 |
| sourcemedia | Server storage Scanned document |
| spelling | 15618 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15618 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 125 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 2020-12-23 MOLECULAR PREVALENCE AND ACUTE PHASE PROTEIN RESPONSE OF Staphylococcus aureus SUBCLINICAL MASTITIS IN GOAT (MASTER_2020).pdf Muhammad Faiq Bin Tajol Ariffin 2020_Molecular Prevalence and Acute Phase Protein Response of Staphylococcus Aureus Subclinical Mastitis in Goat Mastitis is one of the major causes of economic loss in dairy industry and mainly caused by a bacterial infection. The objectives of this study were to determine the molecular prevalence of S. aureus subclinical mastitis and to determine the acute phase response of haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) in serum and milk with relation to somatic cell count (SCC) during experimentally induced subclinical mastitis in goats. A total of 111 goats were screened for mastitis using the California Mastitis Test (CMT). Phenotypic identification of S. aureus was done. The genotypic identification was conducted by Polymerase Chain Reaction (PCR) to detect the nuc gene. Ten goats were included and divided into two group of five goats each. 1 ml inoculum containing 1.2 x 108 cfu of S. aureus was inoculated intracisternally into the mammary gland of five goats. The five other goats were infused with 1 ml of sterile phosphate- buffered saline to serve as a control. Serum and milk concentrations of Hp, SAA and AGP were evaluated pre- and post-inoculation (6, 24, 48 and 72 hours) using ELISA kits. The SCC was measured by direct microscopic method. The SCC was assessed by direct microscopic method. The prevalence of S. aureus subclinical mastitis was 42% (8/19). Serum and milk Hp concentrations increased significantly up to 72 hours compared to the control (P<0.05). The mean concentration of Hp was significantly higher (P<0.05) in the milk than the serum. Serum SAA elevated but significantly (P<0.05) at 6 hours only in infected group. Whereas, the concentration of SAA in milk of infected group was significantly increased (P<0.05) at 6, 24 and 72 hours than in controls. Serum AGP concentrations reached peak levels at 48 hours post inoculation. Milk AGP concentration was significantly increased at 24 hours (P<0.05) and remained significantly elevated until 72 hours compared to the control. A significant correlation between milk Hp concentration and SCC was observed (r= 0.56, P< 0.05). There were no associations between milk SAA and AGP concentrations with SCC. It can be concluded from this study that Hp is a more sensitive indicator for subclinical mastitis and maybe useful for early detection S. aureus subclinical mastitis. Molecular prevalence Prevalence Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Molecular Biology Bacterial Diseases Thesis |
| spellingShingle | 2020_Molecular Prevalence and Acute Phase Protein Response of Staphylococcus Aureus Subclinical Mastitis in Goat |
| state | Terengganu |
| subject | Molecular prevalence Dissertations, Academic |
| summary | Mastitis is one of the major causes of economic loss in dairy industry and mainly caused by a bacterial infection. The objectives of this study were to determine the molecular prevalence of S. aureus subclinical mastitis and to determine the acute phase response of haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) in serum and milk with relation to somatic cell count (SCC) during experimentally induced subclinical mastitis in goats. A total of 111 goats were screened for mastitis using the California Mastitis Test (CMT). Phenotypic identification of S. aureus was done. The genotypic identification was conducted by Polymerase Chain Reaction (PCR) to detect the nuc gene. Ten goats were included and divided into two group of five goats each. 1 ml inoculum containing 1.2 x 108 cfu of S. aureus was inoculated intracisternally into the mammary gland of five goats. The five other goats were infused with 1 ml of sterile phosphate- buffered saline to serve as a control. Serum and milk concentrations of Hp, SAA and AGP were evaluated pre- and post-inoculation (6, 24, 48 and 72 hours) using ELISA kits. The SCC was measured by direct microscopic method. The SCC was assessed by direct microscopic method. The prevalence of S. aureus subclinical mastitis was 42% (8/19). Serum and milk Hp concentrations increased significantly up to 72 hours compared to the control (P<0.05). The mean concentration of Hp was significantly higher (P<0.05) in the milk than the serum. Serum SAA elevated but significantly (P<0.05) at 6 hours only in infected group. Whereas, the concentration of SAA in milk of infected group was significantly increased (P<0.05) at 6, 24 and 72 hours than in controls. Serum AGP concentrations reached peak levels at 48 hours post inoculation. Milk AGP concentration was significantly increased at 24 hours (P<0.05) and remained significantly elevated until 72 hours compared to the control. A significant correlation between milk Hp concentration and SCC was observed (r= 0.56, P< 0.05). There were no associations between milk SAA and AGP concentrations with SCC. It can be concluded from this study that Hp is a more sensitive indicator for subclinical mastitis and maybe useful for early detection S. aureus subclinical mastitis. |
| title | 2020_Molecular Prevalence and Acute Phase Protein Response of Staphylococcus Aureus Subclinical Mastitis in Goat |
| title_full | 2020_Molecular Prevalence and Acute Phase Protein Response of Staphylococcus Aureus Subclinical Mastitis in Goat |
| title_fullStr | 2020_Molecular Prevalence and Acute Phase Protein Response of Staphylococcus Aureus Subclinical Mastitis in Goat |
| title_full_unstemmed | 2020_Molecular Prevalence and Acute Phase Protein Response of Staphylococcus Aureus Subclinical Mastitis in Goat |
| title_short | 2020_Molecular Prevalence and Acute Phase Protein Response of Staphylococcus Aureus Subclinical Mastitis in Goat |
| title_sort | 2020_molecular prevalence and acute phase protein response of staphylococcus aureus subclinical mastitis in goat |