2024_Integrated Environmental DNA (eDNA) And Geographic Information System (GIS) Analysis in Evaluating Habitat Distribution of Redclaw Crayfish (Cherax quadricarinatus)
| Format: | General Document |
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| building | INTELEK Repository |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2024-00-00 |
| format | General Document |
| id | 15614 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | INTEGR~1.PDF |
| person | Mohamad Zulkarnain Bin Mohd Dali |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15614 |
| sourcemedia | Server storage Scanned document |
| spelling | 15614 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15614 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 108 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 3.0.10; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 2024-00-00 INTEGR~1.PDF Mohamad Zulkarnain Bin Mohd Dali Environmental DNA (eDNA) Redclaw crayfish or Cherax quadricarinatus as an invasive species is considered a serious threat to freshwater ecosystems throughout the world. Its presence brings along ecosystem imbalances and causes socioeconomic lose in invaded areas. The information on the presence of C. quadricarinatus is pivotal to monitor the sustainability of biodiversity and ecosystems. However, the assessments on C. quadricarinatus presence are time, resources and energy consuming. Thus, the objective of this study is to evaluate the efficacy of utilising a combination of environmental DNA (eDNA) analysis and Geographic Information System (GIS) mapping as a novel and reliable tool for identification and mapping the distribution of C. quadricarinatus. This study was done at Felda Tenang, Besut, Terengganu where there were known populations of wild C. quadricarinatus. The geographical and habitat-specific features of C. quadricarinatus were analysed using GIS data to project their potential dispersal area. Water samples were then taken at identified sites for eDNA analysis to confirm the presence of redclaw crayfish in expected sites. The eDNA analysis was done using species-specific primer and probe set both through traditional Polymerase Chain Reaction (PCR) and quantitative PCR (qPCR) method. Finally, with the secondary data freely available on the Internet containing the historical record of C. quadricarinatus presence, physical survey and the climate history and pattern in the study area were then compared with the generated database design to identify possible relationship for C. quadricarinatus dispersal. The study area was divided into 4 sampling zonation; namely the Red, Yellow, Blue and Green Zones. The zonation was divided using GIS based on their geographical terrain and elevation above sea level, for which the ranges are: Red (20-30 m), Blue (31-35 m), Yellow (36-39 m) and Green (40-50 m). Traditional PCR detects DNA traces of C. quadricarinatus at only the Red Zone meanwhile qPCR results detected DNA traces from all sampling zonation. The qPCR result indicates 91.67% of the study area to be invaded. Together with Kappa coeeficient of 0.816 in this study, it implores the need for future mitigation plans to curb their dispersal into new areas. The findings also shows that there are fewer but older C. quadricarinatus in streams and drainages at higher elevated residential area but more quantity of younger C. quadricarinatus at lower elevated residential areas. The use of eDNA analysis and GIS very effective in the detection of C. quadricarinatus, allowing to ascertain their dispersal tendencies and prospective distribution area at Felda Tenang. Since there are no specific monitoring methods previously, this integrated technique can be proposed as part of new fisheries management methods. The concept and findings of this research will also contribute to the information in monitoring and managing C. quadricarinatus and other invasive species. 2024_Integrated Environmental DNA (eDNA) And Geographic Information System (GIS) Analysis in Evaluating Habitat Distribution of Redclaw Crayfish (Cherax quadricarinatus) Environmental DNA (eDNA) Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Cherax Quadricarinatus Invasive Species Freshwater Ecosystem Monitoring Thesis |
| spellingShingle | 2024_Integrated Environmental DNA (eDNA) And Geographic Information System (GIS) Analysis in Evaluating Habitat Distribution of Redclaw Crayfish (Cherax quadricarinatus) |
| state | Terengganu |
| subject | Environmental DNA (eDNA) Dissertations, Academic |
| summary | Redclaw crayfish or Cherax quadricarinatus as an invasive species is considered a serious threat to freshwater ecosystems throughout the world. Its presence brings along ecosystem imbalances and causes socioeconomic lose in invaded areas. The information on the presence of C. quadricarinatus is pivotal to monitor the sustainability of biodiversity and ecosystems. However, the assessments on C. quadricarinatus presence are time, resources and energy consuming. Thus, the objective of this study is to evaluate the efficacy of utilising a combination of environmental DNA (eDNA) analysis and Geographic Information System (GIS) mapping as a novel and reliable tool for identification and mapping the distribution of C. quadricarinatus. This study was done at Felda Tenang, Besut, Terengganu where there were known populations of wild C. quadricarinatus. The geographical and habitat-specific features of C. quadricarinatus were analysed using GIS data to project their potential dispersal area. Water samples were then taken at identified sites for eDNA analysis to confirm the presence of redclaw crayfish in expected sites. The eDNA analysis was done using species-specific primer and probe set both through traditional Polymerase Chain Reaction (PCR) and quantitative PCR (qPCR) method. Finally, with the secondary data freely available on the Internet containing the historical record of C. quadricarinatus presence, physical survey and the climate history and pattern in the study area were then compared with the generated database design to identify possible relationship for C. quadricarinatus dispersal. The study area was divided into 4 sampling zonation; namely the Red, Yellow, Blue and Green Zones. The zonation was divided using GIS based on their geographical terrain and elevation above sea level, for which the ranges are: Red (20-30 m), Blue (31-35 m), Yellow (36-39 m) and Green (40-50 m). Traditional PCR detects DNA traces of C. quadricarinatus at only the Red Zone meanwhile qPCR results detected DNA traces from all sampling zonation. The qPCR result indicates 91.67% of the study area to be invaded. Together with Kappa coeeficient of 0.816 in this study, it implores the need for future mitigation plans to curb their dispersal into new areas. The findings also shows that there are fewer but older C. quadricarinatus in streams and drainages at higher elevated residential area but more quantity of younger C. quadricarinatus at lower elevated residential areas. The use of eDNA analysis and GIS very effective in the detection of C. quadricarinatus, allowing to ascertain their dispersal tendencies and prospective distribution area at Felda Tenang. Since there are no specific monitoring methods previously, this integrated technique can be proposed as part of new fisheries management methods. The concept and findings of this research will also contribute to the information in monitoring and managing C. quadricarinatus and other invasive species. |
| title | 2024_Integrated Environmental DNA (eDNA) And Geographic Information System (GIS) Analysis in Evaluating Habitat Distribution of Redclaw Crayfish (Cherax quadricarinatus) |
| title_full | 2024_Integrated Environmental DNA (eDNA) And Geographic Information System (GIS) Analysis in Evaluating Habitat Distribution of Redclaw Crayfish (Cherax quadricarinatus) |
| title_fullStr | 2024_Integrated Environmental DNA (eDNA) And Geographic Information System (GIS) Analysis in Evaluating Habitat Distribution of Redclaw Crayfish (Cherax quadricarinatus) |
| title_full_unstemmed | 2024_Integrated Environmental DNA (eDNA) And Geographic Information System (GIS) Analysis in Evaluating Habitat Distribution of Redclaw Crayfish (Cherax quadricarinatus) |
| title_short | 2024_Integrated Environmental DNA (eDNA) And Geographic Information System (GIS) Analysis in Evaluating Habitat Distribution of Redclaw Crayfish (Cherax quadricarinatus) |
| title_sort | 2024_integrated environmental dna (edna) and geographic information system (gis) analysis in evaluating habitat distribution of redclaw crayfish (cherax quadricarinatus) |