2024_Influence of Nectar Sources on The Physicochemical Property, Antioxidant Capacity, Mineral Content and Anti-Bacterial Activity of Stingless Bee Honey
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| country | Malaysia |
| date | 2024-07-01 |
| format | General Document |
| id | 15613 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | 15613_424e0e23d3c1051.pdf |
| person | Izzati Shahira Binti Rosidi Sujanto |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15613 |
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| spelling | 15613 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15613 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 3.0.10; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Copyright©PWB2025 2024-07-01 15613_424e0e23d3c1051.pdf 156 Izzati Shahira Binti Rosidi Sujanto 2024_Influence of Nectar Sources on The Physicochemical Property, Antioxidant Capacity, Mineral Content and Anti-Bacterial Activity of Stingless Bee Honey The deep interest in the use of stingless bee honey (SBH) as a functional food is due to its composition, which has been associated with its medicinal properties. However, the composition of SBH varies depending on the type and origin of the bees’ diet. The demand for SBH is expanding worldwide, but information on its value as a functional food is still scarce. Hence, this research aimed to determine the impact of different locations on the physicochemical properties and mineral content of SBH, to identify the nectar sources of SBH at different locations and bee species by DNA barcoding and to determine the relationship between nectar sources and the antibacterial activity of SBH. Honey produced by stingless bee H. itama was collected from apiaries located at Manir (MR), Mardi (MD), Besut (BS), UniSZA (UN) and Telaga papan (TP). In MR, SBH produced by H. erytrogastra and G. thorasica were also collected. All locations surrounded with different types of plants; where stingless bees are allowed to collect nectars freely. DNA extraction by the CTAB method was used to identify the nectar sources of honey, followed by amplification and sequencing of DNA. Physicochemical properties and antioxidants by DPPH assay were also evaluated. The total phenolic was analyzed by Folin-Ciocalteu assay and total flavonoid was analyzed by Aluminum Chloride colorimetric assay. ICP-OES instrument is used for the determination of selected minerals; calcium, magnesium, sodium, iron, zinc, copper, aluminum, phosphorus and manganese. Antibacterial activity of SBH were carried out through disc diffusion assay and its activity at different honey percentages.The nectar sources of SBH collected from TP was Melaleuca spp. and UN was Acacia spp. Meanwhile, all SBH samples from MR were multifloral. The result shows that SBH at all locations was acidic, ranging from 3.30 to 3.54. SBH from TP had the highest total soluble solid (75.76 °Brix) compared to others. The moisture of SBH ranged from 27.28 to 33.13% and electrical conductivity of 0.65 to 1.46 mS/cm which significantly differs between locations. For color analysis, the L* values that referred to the brightness of the sample ranged from 25.02 to 52.55. The total flavonoid content of SBH ranged from 1.41 to 6.64 mg CE/g, while the total phenolic content ranged from 3.94 to 10.54 mg GAE/g. For antioxidants by DPPH assay, the value ranges from 71.14 to 85.27% in all honey concentrations. Minerals content resulted in K being the most abundant element in all samples analyzed, with values ranging from 2551.77 to 3688.35 mg/L followed by Na, Ca and Mg. For disc diffusion screening assay, S. enteritidis and S. typhimurium exhibit the highest inhibition zone followed by E. coli, V. parahaemolyticus, S. aureus, L. monocytogenes, and P. aeruginosa. These findings also suggest the use of 20% concentration of SBH would be sufficient to kill the bacteria within 24 hours. In summary, the study demonstrates the impact of nectar sources on the physicochemical properties, minerals and antibacterial activity of SBH. Nectar sources Nectar Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Plant-Pollinator Interactions Stingless Bees Thesis |
| spellingShingle | 2024_Influence of Nectar Sources on The Physicochemical Property, Antioxidant Capacity, Mineral Content and Anti-Bacterial Activity of Stingless Bee Honey |
| state | Terengganu |
| subject | Nectar sources Dissertations, Academic |
| summary | The deep interest in the use of stingless bee honey (SBH) as a functional food is due to its composition, which has been associated with its medicinal properties. However, the composition of SBH varies depending on the type and origin of the bees’ diet. The demand for SBH is expanding worldwide, but information on its value as a functional food is still scarce. Hence, this research aimed to determine the impact of different locations on the physicochemical properties and mineral content of SBH, to identify the nectar sources of SBH at different locations and bee species by DNA barcoding and to determine the relationship between nectar sources and the antibacterial activity of SBH. Honey produced by stingless bee H. itama was collected from apiaries located at Manir (MR), Mardi (MD), Besut (BS), UniSZA (UN) and Telaga papan (TP). In MR, SBH produced by H. erytrogastra and G. thorasica were also collected. All locations surrounded with different types of plants; where stingless bees are allowed to collect nectars freely. DNA extraction by the CTAB method was used to identify the nectar sources of honey, followed by amplification and sequencing of DNA. Physicochemical properties and antioxidants by DPPH assay were also evaluated. The total phenolic was analyzed by Folin-Ciocalteu assay and total flavonoid was analyzed by Aluminum Chloride colorimetric assay. ICP-OES instrument is used for the determination of selected minerals; calcium, magnesium, sodium, iron, zinc, copper, aluminum, phosphorus and manganese. Antibacterial activity of SBH were carried out through disc diffusion assay and its activity at different honey percentages.The nectar sources of SBH collected from TP was Melaleuca spp. and UN was Acacia spp. Meanwhile, all SBH samples from MR were multifloral. The result shows that SBH at all locations was acidic, ranging from 3.30 to 3.54. SBH from TP had the highest total soluble solid (75.76 °Brix) compared to others. The moisture of SBH ranged from 27.28 to 33.13% and electrical conductivity of 0.65 to 1.46 mS/cm which significantly differs between locations. For color analysis, the L* values that referred to the brightness of the sample ranged from 25.02 to 52.55. The total flavonoid content of SBH ranged from 1.41 to 6.64 mg CE/g, while the total phenolic content ranged from 3.94 to 10.54 mg GAE/g. For antioxidants by DPPH assay, the value ranges from 71.14 to 85.27% in all honey concentrations. Minerals content resulted in K being the most abundant element in all samples analyzed, with values ranging from 2551.77 to 3688.35 mg/L followed by Na, Ca and Mg. For disc diffusion screening assay, S. enteritidis and S. typhimurium exhibit the highest inhibition zone followed by E. coli, V. parahaemolyticus, S. aureus, L. monocytogenes, and P. aeruginosa. These findings also suggest the use of 20% concentration of SBH would be sufficient to kill the bacteria within 24 hours. In summary, the study demonstrates the impact of nectar sources on the physicochemical properties, minerals and antibacterial activity of SBH. |
| title | 2024_Influence of Nectar Sources on The Physicochemical Property, Antioxidant Capacity, Mineral Content and Anti-Bacterial Activity of Stingless Bee Honey |
| title_full | 2024_Influence of Nectar Sources on The Physicochemical Property, Antioxidant Capacity, Mineral Content and Anti-Bacterial Activity of Stingless Bee Honey |
| title_fullStr | 2024_Influence of Nectar Sources on The Physicochemical Property, Antioxidant Capacity, Mineral Content and Anti-Bacterial Activity of Stingless Bee Honey |
| title_full_unstemmed | 2024_Influence of Nectar Sources on The Physicochemical Property, Antioxidant Capacity, Mineral Content and Anti-Bacterial Activity of Stingless Bee Honey |
| title_short | 2024_Influence of Nectar Sources on The Physicochemical Property, Antioxidant Capacity, Mineral Content and Anti-Bacterial Activity of Stingless Bee Honey |
| title_sort | 2024_influence of nectar sources on the physicochemical property, antioxidant capacity, mineral content and anti-bacterial activity of stingless bee honey |