2022_In VItro Anti-Tumor Activities and Phytochemical Profile of Propolis Extracts from Different Species of Stingless Bee
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
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| country | Malaysia |
| date | 2022-07-07 |
| format | General Document |
| id | 15612 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | 15612_50aed616370e16e.pdf |
| person | Nor Elani Binti Mat Nafi |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15612 |
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| spelling | 15612 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15612 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 229 Copyright©PWB2025 2022-07-07 15612_50aed616370e16e.pdf Nor Elani Binti Mat Nafi Propolis is a naturally derived resinous materials mixture collected by stingless bee from numerous sources, as well as plant resins with amalgamation of their saliva and wax. It is already knowing since the ancient times in diseases treatments and is the main sources of bioactive natural compounds and as therapeutics agents. Several studies have been conducted and many findings have been reported about the different species of stingless bee containing different groups of compounds. However, the different types of stingless bee species and their biological activities against cancer cells and phytochemical profile has not yet been explored. This study was focused on cytotoxicity screening effect propolis extracts from stingless bee species namely Heterotrigona itama (HI), Geniotrigona thoracica (GT), Lepidotrigona terminata (LT), and Tretrigona apicalis (TA) against different types of cancer cell lines which are uterine fibroid (SK-UT-1), breast cancers (MDA-MB 231 and MCF-7) and cervical cancer (HeLa). Besides, this study also provided the screening of the anti-proliferation effect, to determine the mode of cell death and its mechanism of action by active propolis extract against potential cancer cell line, also to evaluate chemical profile of propolis extracts. Cytotoxicity activity and anti proliferation effects were evaluated by using MTT assay against cancer cell lines. The morphological features and quantitative assay of apoptotic activity were determined via fluorescence microscopy and flow cytometric analysis, respectively. The molecular mechanism of active extract was evaluated by apoptotic related protein expression analysis via Western blotting. The chemical profile of propolis extracts was evaluate by High Performance Thin Layer Chromatography (HPTLC) analysis. H. itama extract showed the highest cytotoxicity effect with IC50 of 4 μg/mL, 5 μg/mL, and 8 μg/mL for SK-UT-1, MDA-MB-231, both MCF-7 and HeLa, respectively. Other species only possessed moderate to weak cytotoxicity effect against the tested cells. The inhibition percentage of SK-UT-1 cell proliferation by active (HI) propolis extract was revealed to be significantly decreased in concentration dependence manner. Morphological analysis revealed the presence of viable cell, apoptotic and necrosis features. AO/PI staining of SK UT-1 cell with the HI extract treatment indicated that majority of cells appeared in the apoptotic cell death mode. The flow cytometric analysis on treated cells showed reduced percentage of cell viability at different concentration (3.125 µg⁄mL to 50 µg⁄mL) at 72 hours. Downregulation of anti-apoptotic protein of BCL-2 showed the same expression with untreated cell, meanwhile increasing the expression of protein IĸB-α. The cleavage of Caspase-3 and PARP-1 which supports the apoptotic activity of HI propolis ethanolic extracts in protein analysis. In addition, this analysis also revealed the presence of Cyclin B1 that plays a crucial role in the cell cycle, respectively. HPTLC showed the abundance of compound presence including saponins, essential oils, terpenoids, phenols, sterols, unsaturated and aromatic compounds in the H. itama as compared to the other extracts. This study has shown that the ethanolic extract of H. itama was the most active extract compared to other species in cytotoxicity activity, reduced proliferation effects towards SK-UT-1 cells and induced the cells into apoptotic cell death mode, also actively involved in the expression of apoptotic-related protein. The data obtained from this finding shown the potential of propolis produced by Heterotrigona itama in treating uterine leiomyosarcoma and worth further investigation. 2022_In VItro Anti-Tumor Activities and Phytochemical Profile of Propolis Extracts from Different Species of Stingless Bee In vitro anti-tumor activities Cancer—Treatment Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Anti-Tumor Agents In Vitro Experiments Thesis |
| spellingShingle | 2022_In VItro Anti-Tumor Activities and Phytochemical Profile of Propolis Extracts from Different Species of Stingless Bee |
| state | Terengganu |
| subject | In vitro anti-tumor activities Dissertations, Academic |
| summary | Propolis is a naturally derived resinous materials mixture collected by stingless bee from numerous sources, as well as plant resins with amalgamation of their saliva and wax. It is already knowing since the ancient times in diseases treatments and is the main sources of bioactive natural compounds and as therapeutics agents. Several studies have been conducted and many findings have been reported about the different species of stingless bee containing different groups of compounds. However, the different types of stingless bee species and their biological activities against cancer cells and phytochemical profile has not yet been explored. This study was focused on cytotoxicity screening effect propolis extracts from stingless bee species namely Heterotrigona itama (HI), Geniotrigona thoracica (GT), Lepidotrigona terminata (LT), and Tretrigona apicalis (TA) against different types of cancer cell lines which are uterine fibroid (SK-UT-1), breast cancers (MDA-MB 231 and MCF-7) and cervical cancer (HeLa). Besides, this study also provided the screening of the anti-proliferation effect, to determine the mode of cell death and its mechanism of action by active propolis extract against potential cancer cell line, also to evaluate chemical profile of propolis extracts. Cytotoxicity activity and anti proliferation effects were evaluated by using MTT assay against cancer cell lines. The morphological features and quantitative assay of apoptotic activity were determined via fluorescence microscopy and flow cytometric analysis, respectively. The molecular mechanism of active extract was evaluated by apoptotic related protein expression analysis via Western blotting. The chemical profile of propolis extracts was evaluate by High Performance Thin Layer Chromatography (HPTLC) analysis. H. itama extract showed the highest cytotoxicity effect with IC50 of 4 μg/mL, 5 μg/mL, and 8 μg/mL for SK-UT-1, MDA-MB-231, both MCF-7 and HeLa, respectively. Other species only possessed moderate to weak cytotoxicity effect against the tested cells. The inhibition percentage of SK-UT-1 cell proliferation by active (HI) propolis extract was revealed to be significantly decreased in concentration dependence manner. Morphological analysis revealed the presence of viable cell, apoptotic and necrosis features. AO/PI staining of SK UT-1 cell with the HI extract treatment indicated that majority of cells appeared in the apoptotic cell death mode. The flow cytometric analysis on treated cells showed reduced percentage of cell viability at different concentration (3.125 µg⁄mL to 50 µg⁄mL) at 72 hours. Downregulation of anti-apoptotic protein of BCL-2 showed the same expression with untreated cell, meanwhile increasing the expression of protein IĸB-α. The cleavage of Caspase-3 and PARP-1 which supports the apoptotic activity of HI propolis ethanolic extracts in protein analysis. In addition, this analysis also revealed the presence of Cyclin B1 that plays a crucial role in the cell cycle, respectively. HPTLC showed the abundance of compound presence including saponins, essential oils, terpenoids, phenols, sterols, unsaturated and aromatic compounds in the H. itama as compared to the other extracts. This study has shown that the ethanolic extract of H. itama was the most active extract compared to other species in cytotoxicity activity, reduced proliferation effects towards SK-UT-1 cells and induced the cells into apoptotic cell death mode, also actively involved in the expression of apoptotic-related protein. The data obtained from this finding shown the potential of propolis produced by Heterotrigona itama in treating uterine leiomyosarcoma and worth further investigation. |
| title | 2022_In VItro Anti-Tumor Activities and Phytochemical Profile of Propolis Extracts from Different Species of Stingless Bee |
| title_full | 2022_In VItro Anti-Tumor Activities and Phytochemical Profile of Propolis Extracts from Different Species of Stingless Bee |
| title_fullStr | 2022_In VItro Anti-Tumor Activities and Phytochemical Profile of Propolis Extracts from Different Species of Stingless Bee |
| title_full_unstemmed | 2022_In VItro Anti-Tumor Activities and Phytochemical Profile of Propolis Extracts from Different Species of Stingless Bee |
| title_short | 2022_In VItro Anti-Tumor Activities and Phytochemical Profile of Propolis Extracts from Different Species of Stingless Bee |
| title_sort | 2022_in vitro anti-tumor activities and phytochemical profile of propolis extracts from different species of stingless bee |