2021_Unravelling the Role of Transient Receptor Potential Vanilloid 4 (TTRPV4) in HT-29 Colorectal Cancer Cells
| Format: | General Document |
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| building | INTELEK Repository |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2022-01-25 |
| format | General Document |
| id | 15539 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | UNRAVELLING THE ROLE OF TRANSIENT RECEPTOR POTENTIAL VANILLOID 4 (TTRPV4) IN HT-29 COLORECTAL CANCER CELLS (MASTER_2021).pdf |
| person | Nurul Nadiah Binti Bahari |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15539 |
| sourcemedia | Server storage Scanned document |
| spelling | 15539 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15539 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 153 UNRAVELLING THE ROLE OF TRANSIENT RECEPTOR POTENTIAL VANILLOID 4 (TTRPV4) IN HT-29 COLORECTAL CANCER CELLS (MASTER_2021).pdf 2021_Unravelling the Role of Transient Receptor Potential Vanilloid 4 (TTRPV4) in HT-29 Colorectal Cancer Cells Copyright©PWB2025 Nurul Nadiah Binti Bahari 2022-01-25 The Transient Receptor Potential Vanilloid 4 (TRPV4) is a cation channel, which is activated by various stimuli. TRPV4 is broadly expressed in tissues such as kidneys, lungs, liver and salivary glands. Altered expression and/or activity of TRPV4 channel have been associated with certain pathological conditions, including cancer. Despite evidence for the role of TRPV4 in several types of cancers, studies assessing potential roles of TRPV4 in the context of colorectal cancer remain limited. Unresponsiveness of some colorectal cancer patients towards currently available molecularly targeted therapies has highlighted the need to find new and alternative drug targets for the treatment of colorectal cancer. This study was intended to evaluate the roles of TRPV4 in colorectal cancer cells, particularly in cell proliferation, cell cycle and cell death. Quantitative Real-Time PCR analysis was performed to determine the TRPV4 mRNA expression levels in two human colorectal cancer cell lines and normal colon cells. A TRPV4 activator, GSK1016790A, and an inhibitor, RN 1734, were used in experiments, which investigated on the effects of TRPV4 pharmacological modulation on cell proliferation, cell cycle and cell death in human colorectal cancer cells. Cell proliferation was assessed using the MTT cell proliferation assay. Flow cytometry analysis was used to evaluate the effects of TRPV4 pharmacological modulators on cell cycle and cell death in human colorectal cancer cells. TRPV4 mRNA expression was significantly lower in HT-29 colorectal cancer cells than those seen in the normal colon CCD-18Co cells, while no expression of TRPV4 was detected in HCT-116 colorectal cancer cells. The activation of TRPV4 using GSK1016790A resulted in significant increase of cell proliferation while TRPV4 inhibition using RN 1734 produced an opposing effect on the proliferation of HT-29 cells. Co-treatment with RN 1734 inhibited GSK1016790A-mediated increases significantly in proliferation of HT-29 cells. Cell cycle analysis revealed no significant effect of TRPV4 pharmacological modulators on the cell cycle profile of HT-29 cells. Both activation and inhibition of TRPV4 channel promoted the death of HT-29 cells, despite at varying degrees of cell death induction, as determined by the Annexin V/PI double staining assay. TRPV4 is differentially expressed in human colorectal cancer cells. Modulation of TRPV4 using its pharmacological modulators appears to alter the proliferation of HT-29 cells and promote cell death in this cell line. These findings suggest that TRPV4 is amenable to therapeutic interventions for the treatment of colorectal cancer. Colorectal cancer—Molecular aspects Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... TRPV4 Channel HT-29 Colorectal Cancer Cells Cancer cell Proliferation Thesis |
| spellingShingle | 2021_Unravelling the Role of Transient Receptor Potential Vanilloid 4 (TTRPV4) in HT-29 Colorectal Cancer Cells |
| state | Terengganu |
| subject | Colorectal cancer—Molecular aspects Dissertations, Academic |
| summary | The Transient Receptor Potential Vanilloid 4 (TRPV4) is a cation channel, which is activated by various stimuli. TRPV4 is broadly expressed in tissues such as kidneys, lungs, liver and salivary glands. Altered expression and/or activity of TRPV4 channel have been associated with certain pathological conditions, including cancer. Despite evidence for the role of TRPV4 in several types of cancers, studies assessing potential roles of TRPV4 in the context of colorectal cancer remain limited. Unresponsiveness of some colorectal cancer patients towards currently available molecularly targeted therapies has highlighted the need to find new and alternative drug targets for the treatment of colorectal cancer. This study was intended to evaluate the roles of TRPV4 in colorectal cancer cells, particularly in cell proliferation, cell cycle and cell death. Quantitative Real-Time PCR analysis was performed to determine the TRPV4 mRNA expression levels in two human colorectal cancer cell lines and normal colon cells. A TRPV4 activator, GSK1016790A, and an inhibitor, RN 1734, were used in experiments, which investigated on the effects of TRPV4 pharmacological modulation on cell proliferation, cell cycle and cell death in human colorectal cancer cells. Cell proliferation was assessed using the MTT cell proliferation assay. Flow cytometry analysis was used to evaluate the effects of TRPV4 pharmacological modulators on cell cycle and cell death in human colorectal cancer cells. TRPV4 mRNA expression was significantly lower in HT-29 colorectal cancer cells than those seen in the normal colon CCD-18Co cells, while no expression of TRPV4 was detected in HCT-116 colorectal cancer cells. The activation of TRPV4 using GSK1016790A resulted in significant increase of cell proliferation while TRPV4 inhibition using RN 1734 produced an opposing effect on the proliferation of HT-29 cells. Co-treatment with RN 1734 inhibited GSK1016790A-mediated increases significantly in proliferation of HT-29 cells. Cell cycle analysis revealed no significant effect of TRPV4 pharmacological modulators on the cell cycle profile of HT-29 cells. Both activation and inhibition of TRPV4 channel promoted the death of HT-29 cells, despite at varying degrees of cell death induction, as determined by the Annexin V/PI double staining assay. TRPV4 is differentially expressed in human colorectal cancer cells. Modulation of TRPV4 using its pharmacological modulators appears to alter the proliferation of HT-29 cells and promote cell death in this cell line. These findings suggest that TRPV4 is amenable to therapeutic interventions for the treatment of colorectal cancer. |
| title | 2021_Unravelling the Role of Transient Receptor Potential Vanilloid 4 (TTRPV4) in HT-29 Colorectal Cancer Cells |
| title_full | 2021_Unravelling the Role of Transient Receptor Potential Vanilloid 4 (TTRPV4) in HT-29 Colorectal Cancer Cells |
| title_fullStr | 2021_Unravelling the Role of Transient Receptor Potential Vanilloid 4 (TTRPV4) in HT-29 Colorectal Cancer Cells |
| title_full_unstemmed | 2021_Unravelling the Role of Transient Receptor Potential Vanilloid 4 (TTRPV4) in HT-29 Colorectal Cancer Cells |
| title_short | 2021_Unravelling the Role of Transient Receptor Potential Vanilloid 4 (TTRPV4) in HT-29 Colorectal Cancer Cells |
| title_sort | 2021_unravelling the role of transient receptor potential vanilloid 4 (ttrpv4) in ht-29 colorectal cancer cells |