2018_The Effects Of 3,4 Methylenedioxymethamphetamine On Body Weight, Serotonin Level And Brain Histology Of Rat
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| country | Malaysia |
| date | 2018-08-09 |
| format | General Document |
| id | 15523 |
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| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | THE EFFECTS OF 3,4- METHYLENEDIOXYMETHAMPHETAMINE ON BODY WEIGHT, SEROTONIN LEVEL AND BRAIN HISTOLOGY OF RAT (MASTER_2018).pdf |
| person | Fatin Haniza Binti Zakaria |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15523 |
| sourcemedia | Server storage Scanned document |
| spelling | 15523 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15523 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 169 THE EFFECTS OF 3,4- METHYLENEDIOXYMETHAMPHETAMINE ON BODY WEIGHT, SEROTONIN LEVEL AND BRAIN HISTOLOGY OF RAT (MASTER_2018).pdf Fatin Haniza Binti Zakaria 3,4-Methylenedioxymethamphetamine—Physiological effect Body Weight 3,4-Methylenedioxymethamphetamine (MDMA) Neuropharmacology 2018_The Effects Of 3,4 Methylenedioxymethamphetamine On Body Weight, Serotonin Level And Brain Histology Of Rat Copyright©PWB2025 Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... 2018-08-09 3, 4-methylenedioxymethamphetamine (MDMA) is a powerful central nervous system stimulant that acts by increasing the activities of several neurotransmitters and neurohormonal systems. The administration of MDMA causes a massive efflux of serotonin level, leading to neuronal damages in the hippocampus. In this study, the effects of different doses of MDMA on the body weight, serotonin level and histological changes in the brains of rats were determined. Male Sprague Dawley rats were divided into four groups i.e. one control and three test groups with different doses of MDMA (5, 10 and 15mg/kg). The control group (n=7) received a daily intraperitoneal (i.p.) injection of 1 ml/gm saline twice a week. Meanwhile, for the MDMA groups (n=19, 6 or 7 per dosage group), the rats received daily i.p. injections of 5, 10 and 15mg/kg of MDMA twice a week. The changes in the body weight of the rats were recorded from day one until day seven. Cerebrospinal fluid (CSF) was collected 24 hours after the last injection and the assessment of serotonin level was carried out by using Enzyme-Link Immunosorbent Assay (ELISA) kit. The assessment of histopathological changes was carried out in the hippocampus area by focusing on three different regions i.e. CA1, CA3, and DG by using Cresyl Echt Violet and Glial Fibrillary Acidic Protein (GFAP) staining. Statistical analysis was conducted by using one-way analysis of variance (ANOVA) and Pearson correlation. P-values less than 0.05 were considered statistically significant. All rat groups injected with MDMA showed significantly increased levels of serotonin in the CSF and reduction in body weights gain as well as the number of intact neurons in the hippocampus of the brain when compared to the control group (p<0.001). It was found that the number of astrocytes in all hippocampus regions increased in all groups of rats injected with MDMA, except for CA1 region of the group administered with 5 mg/kg of MDMA. There were also significant correlations between serotonin level with body weight of rats, number of intact neurons and astrocytes (p<0.001). It was concluded that the administration of MDMA in rats elevated the serotonin level in the CSF, thus affecting the body weight, the number of intact neurons, and astrocytes. This study also showed that there were correlations between serotonin level, body weight, and histopathological changes in the hippocampus regions of the rat brain after MDMA administration. Thesis |
| spellingShingle | 2018_The Effects Of 3,4 Methylenedioxymethamphetamine On Body Weight, Serotonin Level And Brain Histology Of Rat |
| state | Terengganu |
| subject | 3,4-Methylenedioxymethamphetamine—Physiological effect Dissertations, Academic |
| summary | 3, 4-methylenedioxymethamphetamine (MDMA) is a powerful central nervous system stimulant that acts by increasing the activities of several neurotransmitters and neurohormonal systems. The administration of MDMA causes a massive efflux of serotonin level, leading to neuronal damages in the hippocampus. In this study, the effects of different doses of MDMA on the body weight, serotonin level and histological changes in the brains of rats were determined. Male Sprague Dawley rats were divided into four groups i.e. one control and three test groups with different doses of MDMA (5, 10 and 15mg/kg). The control group (n=7) received a daily intraperitoneal (i.p.) injection of 1 ml/gm saline twice a week. Meanwhile, for the MDMA groups (n=19, 6 or 7 per dosage group), the rats received daily i.p. injections of 5, 10 and 15mg/kg of MDMA twice a week. The changes in the body weight of the rats were recorded from day one until day seven. Cerebrospinal fluid (CSF) was collected 24 hours after the last injection and the assessment of serotonin level was carried out by using Enzyme-Link Immunosorbent Assay (ELISA) kit. The assessment of histopathological changes was carried out in the hippocampus area by focusing on three different regions i.e. CA1, CA3, and DG by using Cresyl Echt Violet and Glial Fibrillary Acidic Protein (GFAP) staining. Statistical analysis was conducted by using one-way analysis of variance (ANOVA) and Pearson correlation. P-values less than 0.05 were considered statistically significant. All rat groups injected with MDMA showed significantly increased levels of serotonin in the CSF and reduction in body weights gain as well as the number of intact neurons in the hippocampus of the brain when compared to the control group (p<0.001). It was found that the number of astrocytes in all hippocampus regions increased in all groups of rats injected with MDMA, except for CA1 region of the group administered with 5 mg/kg of MDMA. There were also significant correlations between serotonin level with body weight of rats, number of intact neurons and astrocytes (p<0.001). It was concluded that the administration of MDMA in rats elevated the serotonin level in the CSF, thus affecting the body weight, the number of intact neurons, and astrocytes. This study also showed that there were correlations between serotonin level, body weight, and histopathological changes in the hippocampus regions of the rat brain after MDMA administration. |
| title | 2018_The Effects Of 3,4 Methylenedioxymethamphetamine On Body Weight, Serotonin Level And Brain Histology Of Rat |
| title_full | 2018_The Effects Of 3,4 Methylenedioxymethamphetamine On Body Weight, Serotonin Level And Brain Histology Of Rat |
| title_fullStr | 2018_The Effects Of 3,4 Methylenedioxymethamphetamine On Body Weight, Serotonin Level And Brain Histology Of Rat |
| title_full_unstemmed | 2018_The Effects Of 3,4 Methylenedioxymethamphetamine On Body Weight, Serotonin Level And Brain Histology Of Rat |
| title_short | 2018_The Effects Of 3,4 Methylenedioxymethamphetamine On Body Weight, Serotonin Level And Brain Histology Of Rat |
| title_sort | 2018_the effects of 3,4 methylenedioxymethamphetamine on body weight, serotonin level and brain histology of rat |