2023_Detection, Antibiotic Susceptibility and Cross-Contamination Study of Vibrio parahaemolyticus in Keropok Lekor in Terengganu
| Format: | General Document |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2023-07-04 |
| format | General Document |
| id | 15507 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | DETECTION, ANTIBIOTIC SUSCEPTIBILITY AND CROSS-CONTAMINATION STUDY OF Vibrio parahaemolyticus IN KEROPOK LEKOR IN TERENGGANU (MASTER_2023).pdf |
| person | Adebisi Naziahsalam Kehinde |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15507 |
| sourcemedia | Server storage Scanned document |
| spelling | 15507 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15507 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 113 DETECTION, ANTIBIOTIC SUSCEPTIBILITY AND CROSS-CONTAMINATION STUDY OF Vibrio parahaemolyticus IN KEROPOK LEKOR IN TERENGGANU (MASTER_2023).pdf Copyright©PWB2025 Vibrio parahaemolyticus 2023-07-04 Vibrio parahaemolyticus is a Gram-negative bacterium that is found in the marine environment and causes acute gastroenteritis in humans. V. parahaemolyticus is an important foodborne pathogen found to contaminate fish and fish products. Thus, this study aims to detect and characterize V. parahaemolyticus isolated from frozen fish snack in Terengganu and to determine the effects of blast and conventional freezing methods on the V. parahaemolyticus in fish snack, deboned sardine and deboned round scad. A total of 120 frozen fish snack samples in Terengganu were examined for the presence of V. parahaemolyticus using the conventional plating method on Thiosulfate Citrate Bile-salt Sucrose (TCBS) agar and Multiplex Polymerase Chain Reaction (PCR) assay for detection of toxR, tdh and trh gene. The V. parahaemolyticus isolates were tested for antimicrobial susceptibility using the disc diffusion method against 12 types of antibiotics and the Multiple Antibiotic Resistance (MAR) index was determined. The survivability of V. parahaemolyticus was carried out on two different food contact surfaces (stainless steel and plastic) and the cross contamination to boiled and fried fish snack was also determined. The blast and conventional freezing methods were applied to fish snack, deboned sardine and deboned round scad. The occurence of V. parahaemolyticus in fish snack was 64.3% in Kuala Besut, 19% in Kuala Nerus and 0% in Setiu, respectively. All the isolates possess the trh gene but not the tdh gene. MAR index of V. parahaemolyticus isolates was between 0.25 and 0.58. There are no significant differences on V. parahaemolyticus survivability and cross contamination on the food contact surfaces (steel and plastic) throughout 6 h incubation. Blast freezing was more effective in reducing V. parahaemolyticus in frozen fish snack than conventional freezing. For deboned sardine and deboned round scad, there was no significant difference between both freezing methods. This study proved that the frozen fish snack was contaminated with multiple antibiotic resistant V. parahaemolyticus. The pathogen can survive on the different food contact surfaces and eventually transferred to cooked fish snack. Blast freezing are important preservation method to ensure the safety of frozen fish snack. 2023_Detection, Antibiotic Susceptibility and Cross-Contamination Study of Vibrio parahaemolyticus in Keropok Lekor in Terengganu Adebisi Naziahsalam Kehinde Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Vibrio Parahaemolyticus Antibiotic Susceptibility Cross-Contamination Thesis |
| spellingShingle | 2023_Detection, Antibiotic Susceptibility and Cross-Contamination Study of Vibrio parahaemolyticus in Keropok Lekor in Terengganu |
| state | Terengganu |
| subject | Vibrio parahaemolyticus Dissertations, Academic |
| summary | Vibrio parahaemolyticus is a Gram-negative bacterium that is found in the marine environment and causes acute gastroenteritis in humans. V. parahaemolyticus is an important foodborne pathogen found to contaminate fish and fish products. Thus, this study aims to detect and characterize V. parahaemolyticus isolated from frozen fish snack in Terengganu and to determine the effects of blast and conventional freezing methods on the V. parahaemolyticus in fish snack, deboned sardine and deboned round scad. A total of 120 frozen fish snack samples in Terengganu were examined for the presence of V. parahaemolyticus using the conventional plating method on Thiosulfate Citrate Bile-salt Sucrose (TCBS) agar and Multiplex Polymerase Chain Reaction (PCR) assay for detection of toxR, tdh and trh gene. The V. parahaemolyticus isolates were tested for antimicrobial susceptibility using the disc diffusion method against 12 types of antibiotics and the Multiple Antibiotic Resistance (MAR) index was determined. The survivability of V. parahaemolyticus was carried out on two different food contact surfaces (stainless steel and plastic) and the cross contamination to boiled and fried fish snack was also determined. The blast and conventional freezing methods were applied to fish snack, deboned sardine and deboned round scad. The occurence of V. parahaemolyticus in fish snack was 64.3% in Kuala Besut, 19% in Kuala Nerus and 0% in Setiu, respectively. All the isolates possess the trh gene but not the tdh gene. MAR index of V. parahaemolyticus isolates was between 0.25 and 0.58. There are no significant differences on V. parahaemolyticus survivability and cross contamination on the food contact surfaces (steel and plastic) throughout 6 h incubation. Blast freezing was more effective in reducing V. parahaemolyticus in frozen fish snack than conventional freezing. For deboned sardine and deboned round scad, there was no significant difference between both freezing methods. This study proved that the frozen fish snack was contaminated with multiple antibiotic resistant V. parahaemolyticus. The pathogen can survive on the different food contact surfaces and eventually transferred to cooked fish snack. Blast freezing are important preservation method to ensure the safety of frozen fish snack. |
| title | 2023_Detection, Antibiotic Susceptibility and Cross-Contamination Study of Vibrio parahaemolyticus in Keropok Lekor in Terengganu |
| title_full | 2023_Detection, Antibiotic Susceptibility and Cross-Contamination Study of Vibrio parahaemolyticus in Keropok Lekor in Terengganu |
| title_fullStr | 2023_Detection, Antibiotic Susceptibility and Cross-Contamination Study of Vibrio parahaemolyticus in Keropok Lekor in Terengganu |
| title_full_unstemmed | 2023_Detection, Antibiotic Susceptibility and Cross-Contamination Study of Vibrio parahaemolyticus in Keropok Lekor in Terengganu |
| title_short | 2023_Detection, Antibiotic Susceptibility and Cross-Contamination Study of Vibrio parahaemolyticus in Keropok Lekor in Terengganu |
| title_sort | 2023_detection, antibiotic susceptibility and cross-contamination study of vibrio parahaemolyticus in keropok lekor in terengganu |