2019_Detection and Characterization of Vibrio Parahaemolyticus in Raw Round Scad (Decapterus SPP.) and Its Products
| Format: | General Document |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2019-12-01 |
| format | General Document |
| id | 15506 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | DETECTION AND CHARACTERIZATION OF VIBRIO PARAHAEMOLYTICUS IN RAW ROUND SCAD (DECAPTERUS SPP.) AND ITS PRODUCTS (MASTER_2019).pdf |
| person | Syamimi Hanim Binti Mat Sa’ad |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15506 |
| sourcemedia | Server storage Scanned document |
| spelling | 15506 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15506 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 179 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Syamimi Hanim Binti Mat Sa’ad 2019-12-01 DETECTION AND CHARACTERIZATION OF VIBRIO PARAHAEMOLYTICUS IN RAW ROUND SCAD (DECAPTERUS SPP.) AND ITS PRODUCTS (MASTER_2019).pdf Copyright©PWB2025 2019_Detection and Characterization of Vibrio Parahaemolyticus in Raw Round Scad (Decapterus SPP.) and Its Products Vibrio parahaemolyticus is a halophilic Gram negative bacterium and naturally inhabits in marine environment. It is one of main foodborne pathogen that can cause gastroenteritis in human through the consumption of raw, undercooked or contaminated seafood. This study aimed to investigate the prevalence of V. parahaemolyticus in raw round scad as well as its products namely satar and otak otak. Polymerase chain reaction (PCR) and conventional plating method were used to detect and isolate V. parahaemolyticus in 120 fish, 90 satar and 35 otak-otak that have been purchased in Terengganu. The isolates were characterized by 12 types of antibiotic using discs diffusion method. Survivability of V. parahaemolyticus in artificially contaminated satar and otak-otak were determined using spread plate method. Biofilm formation of V. parahaemolyticus on stainless steel, glass and plastic surfaces was assessed using crystal violet assay. Approximately 67.5%, 23.3% and 5.71% of fish, satar and otak-otak were contaminated with V. parahaemolyticus, respectively. A total of 58 V. parahaemolyticus isolates were recovered from fish. Among them, 24 of V. parahaemolyticus isolates carried tdh gene and 3 isolates had trh gene. All the isolates were susceptible to amikacin, norfloxacin, ceftazidime, ciprofloxacin, and enrofloxacin. There was high resistant towards penicillin (93.1%), vancomycin (89.7%) and ampicillin (55.2%). About 56.9% of strains isolated showed a MAR index higher than 0.2, indicating these strains were from high risk sources of contamination. Survivability of V. parahaemolyticus in satar and otak-otak inoculated with approximately 8.66 log CFU/mL found decreasing number of V. parahaemolyticus with the increasing incubation time. For biofilm formation, the readings of OD570 at time 96 h for fish broth growth medium on stainless steel, glass and plastic were 0.76±0.04, 1.38±0.03 and 0.96±0.04, respectively, indicating fish broth could support V. parahaemolyticus biofilm formation. The results showed that the attachment of V. parahaemolyticus cells on these contact surfaces increases with time. This study found V. parahaemolyticus contaminated raw round scad, capable to survive in satar and otak-otak and form biofilm on different food contact surfaces. Vibrio parahaemolyticus Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Vibrio Parahaemolyticus Molecular Characterization Detection Methods Thesis |
| spellingShingle | 2019_Detection and Characterization of Vibrio Parahaemolyticus in Raw Round Scad (Decapterus SPP.) and Its Products |
| state | Terengganu |
| subject | Vibrio parahaemolyticus Dissertations, Academic |
| summary | Vibrio parahaemolyticus is a halophilic Gram negative bacterium and naturally inhabits in marine environment. It is one of main foodborne pathogen that can cause gastroenteritis in human through the consumption of raw, undercooked or contaminated seafood. This study aimed to investigate the prevalence of V. parahaemolyticus in raw round scad as well as its products namely satar and otak otak. Polymerase chain reaction (PCR) and conventional plating method were used to detect and isolate V. parahaemolyticus in 120 fish, 90 satar and 35 otak-otak that have been purchased in Terengganu. The isolates were characterized by 12 types of antibiotic using discs diffusion method. Survivability of V. parahaemolyticus in artificially contaminated satar and otak-otak were determined using spread plate method. Biofilm formation of V. parahaemolyticus on stainless steel, glass and plastic surfaces was assessed using crystal violet assay. Approximately 67.5%, 23.3% and 5.71% of fish, satar and otak-otak were contaminated with V. parahaemolyticus, respectively. A total of 58 V. parahaemolyticus isolates were recovered from fish. Among them, 24 of V. parahaemolyticus isolates carried tdh gene and 3 isolates had trh gene. All the isolates were susceptible to amikacin, norfloxacin, ceftazidime, ciprofloxacin, and enrofloxacin. There was high resistant towards penicillin (93.1%), vancomycin (89.7%) and ampicillin (55.2%). About 56.9% of strains isolated showed a MAR index higher than 0.2, indicating these strains were from high risk sources of contamination. Survivability of V. parahaemolyticus in satar and otak-otak inoculated with approximately 8.66 log CFU/mL found decreasing number of V. parahaemolyticus with the increasing incubation time. For biofilm formation, the readings of OD570 at time 96 h for fish broth growth medium on stainless steel, glass and plastic were 0.76±0.04, 1.38±0.03 and 0.96±0.04, respectively, indicating fish broth could support V. parahaemolyticus biofilm formation. The results showed that the attachment of V. parahaemolyticus cells on these contact surfaces increases with time. This study found V. parahaemolyticus contaminated raw round scad, capable to survive in satar and otak-otak and form biofilm on different food contact surfaces. |
| title | 2019_Detection and Characterization of Vibrio Parahaemolyticus in Raw Round Scad (Decapterus SPP.) and Its Products |
| title_full | 2019_Detection and Characterization of Vibrio Parahaemolyticus in Raw Round Scad (Decapterus SPP.) and Its Products |
| title_fullStr | 2019_Detection and Characterization of Vibrio Parahaemolyticus in Raw Round Scad (Decapterus SPP.) and Its Products |
| title_full_unstemmed | 2019_Detection and Characterization of Vibrio Parahaemolyticus in Raw Round Scad (Decapterus SPP.) and Its Products |
| title_short | 2019_Detection and Characterization of Vibrio Parahaemolyticus in Raw Round Scad (Decapterus SPP.) and Its Products |
| title_sort | 2019_detection and characterization of vibrio parahaemolyticus in raw round scad (decapterus spp.) and its products |