2017_Chemical composition and Biological activities of Heterotrigona itama and Geniotrigona Thoracica Propolis from Malaysia
| Format: | General Document |
|---|
| _version_ | 1860798018436988928 |
|---|---|
| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2018-01-29 |
| format | General Document |
| id | 15493 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITIES OF HETEROTRIGONA ITAMA AND GENIOTRIGONA THORACICA PROPOLIS FROM MALAYSIA (MASTER_2018).pdf |
| person | Nurhamizah Binti Ibrahim |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15493 |
| sourcemedia | Server storage Scanned document |
| spelling | 15493 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15493 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 176 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Geniotrigona thoracica Propolis 2018-01-29 CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITIES OF HETEROTRIGONA ITAMA AND GENIOTRIGONA THORACICA PROPOLIS FROM MALAYSIA (MASTER_2018).pdf Nurhamizah Binti Ibrahim Copyright©PWB2025 2017_Chemical composition and Biological activities of Heterotrigona itama and Geniotrigona Thoracica Propolis from Malaysia The chemical composition of propolis is highly variable and complex depends on the bee species at the sampling location. However, data on comparison of chemical properties between Malaysian stingless bee species was not found at the time of this research. It is crucial to compare the differences of the quality of propolis from different species through the examination on physicochemical characteristics, biological activity and chemical composition before developing propolis as a therapeutic agent or value-added products. This study was performed to evaluate and compare the nutritional value, chemical composition and biological activity of Malaysian stingless bee propolis of two species, Heterotrigona itama (HI) and Geniotrigona thoracica (GT). The nutritional value, the content of moisture, ash, crude protein, fat, fibre and carbohydrate was determined. The spectroscopic technique namely Fourier Transform Infrared (FTIR) and chromatographic techniques, which were High Performance Thin Layer Chromatography (HPTLC) and Gas Chromatography-Mass Spectrometry (GC-MS) were used to establish chemical profile. FTIR and HPTLC data were subjected to chemometrics analysis such as Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) to evaluate the discrimination between samples. Proximate value analysis showed HI had more moisture content, crude protein and carbohydrate compared to that of GT. On the other hand, GT contained more fibre compared to HI. Propolis from HI also had a higher content of total flavonoid and phenolic compounds. The chemical profile of propolis showed a clear separation between samples in different species. Principal component analysis of HPTLC showed HI propolis were differ from GT propolis with their PC1 and PC2 values of 51.26% and 46.74%, respectively at 254 nm. While at 366 nm, their PC1 and PC2 values of 47.84% and 44.68%, respectively. HPTLC confirmed the two clusters established from HCA grouping of HI and GT propolis for both extracts. PCA of FTIR showed that ethanol extract for both species clustered quite similarly but in methanol extract were clustered apart from each other with their PC1 and PC2 values of 54.01% and 41.61%. However, HCA grouping of FTIR were confirmed that two clusters were established of HI and GT propolis for both extracts. In biological analysis, HI propolis exhibited stronger antioxidant activity through DPPH free radical scavenging activity for methanol and ethanol extract with their IC50 values of 10 μg/mL dan 20 μg/mL compared to GT propolis for methanol and ethanol extract with their IC50 values of 270 μg/mL dan 400 μg/mL. HI also exhibited stronger antibacterial activity compared to GT extracts. In conclusion, data gathered in this study revealed that bee species play a role in collecting propolis as shown by nutritional, chemical and biological profile of propolis and should put into account in decision of further development for propolis. Propolis Heterotrigona itama Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Thesis |
| spellingShingle | 2017_Chemical composition and Biological activities of Heterotrigona itama and Geniotrigona Thoracica Propolis from Malaysia |
| state | Terengganu |
| subject | Propolis Dissertations, Academic |
| summary | The chemical composition of propolis is highly variable and complex depends on the bee species at the sampling location. However, data on comparison of chemical properties between Malaysian stingless bee species was not found at the time of this research. It is crucial to compare the differences of the quality of propolis from different species through the examination on physicochemical characteristics, biological activity and chemical composition before developing propolis as a therapeutic agent or value-added products. This study was performed to evaluate and compare the nutritional value, chemical composition and biological activity of Malaysian stingless bee propolis of two species, Heterotrigona itama (HI) and Geniotrigona thoracica (GT). The nutritional value, the content of moisture, ash, crude protein, fat, fibre and carbohydrate was determined. The spectroscopic technique namely Fourier Transform Infrared (FTIR) and chromatographic techniques, which were High Performance Thin Layer Chromatography (HPTLC) and Gas Chromatography-Mass Spectrometry (GC-MS) were used to establish chemical profile. FTIR and HPTLC data were subjected to chemometrics analysis such as Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) to evaluate the discrimination between samples. Proximate value analysis showed HI had more moisture content, crude protein and carbohydrate compared to that of GT. On the other hand, GT contained more fibre compared to HI. Propolis from HI also had a higher content of total flavonoid and phenolic compounds. The chemical profile of propolis showed a clear separation between samples in different species. Principal component analysis of HPTLC showed HI propolis were differ from GT propolis with their PC1 and PC2 values of 51.26% and 46.74%, respectively at 254 nm. While at 366 nm, their PC1 and PC2 values of 47.84% and 44.68%, respectively. HPTLC confirmed the two clusters established from HCA grouping of HI and GT propolis for both extracts. PCA of FTIR showed that ethanol extract for both species clustered quite similarly but in methanol extract were clustered apart from each other with their PC1 and PC2 values of 54.01% and 41.61%. However, HCA grouping of FTIR were confirmed that two clusters were established of HI and GT propolis for both extracts. In biological analysis, HI propolis exhibited stronger antioxidant activity through DPPH free radical scavenging activity for methanol and ethanol extract with their IC50 values of 10 μg/mL dan 20 μg/mL compared to GT propolis for methanol and ethanol extract with their IC50 values of 270 μg/mL dan 400 μg/mL. HI also exhibited stronger antibacterial activity compared to GT extracts. In conclusion, data gathered in this study revealed that bee species play a role in collecting propolis as shown by nutritional, chemical and biological profile of propolis and should put into account in decision of further development for propolis. |
| title | 2017_Chemical composition and Biological activities of Heterotrigona itama and Geniotrigona Thoracica Propolis from Malaysia |
| title_full | 2017_Chemical composition and Biological activities of Heterotrigona itama and Geniotrigona Thoracica Propolis from Malaysia |
| title_fullStr | 2017_Chemical composition and Biological activities of Heterotrigona itama and Geniotrigona Thoracica Propolis from Malaysia |
| title_full_unstemmed | 2017_Chemical composition and Biological activities of Heterotrigona itama and Geniotrigona Thoracica Propolis from Malaysia |
| title_short | 2017_Chemical composition and Biological activities of Heterotrigona itama and Geniotrigona Thoracica Propolis from Malaysia |
| title_sort | 2017_chemical composition and biological activities of heterotrigona itama and geniotrigona thoracica propolis from malaysia |