2021_Characterization and Biological Functions of Trichoderma hamatum, T. harzianum and T. atroviride As A Biological Control agent, Biodecomposer and Plant Growth Promoter
| Format: | General Document |
|---|
| _version_ | 1860798018000781312 |
|---|---|
| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2021-10-25 |
| format | General Document |
| id | 15491 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | CHARAC~1.PDF |
| person | Nur Aisyah Zin |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15491 |
| sourcemedia | Server storage Scanned document |
| spelling | 15491 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15491 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 21 SAMBox 2.3.4 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN 2021-10-25 CHARAC~1.PDF Nur Aisyah Zin Copyright©PWB2025 2021_Characterization and Biological Functions of Trichoderma hamatum, T. harzianum and T. atroviride As A Biological Control agent, Biodecomposer and Plant Growth Promoter Trichoderma spp. have been widely used in agricultural application due to its well known biological control mechanism. The usage of Trichoderma-based products attracts the attention of researchers to discover more on other potential benefits of Trichoderma spp. mainly in issues concerning the food system including the use of excessive pesticide and chemical fertilizer. Besides, improper management of food wastes is also a rising problem recently which has lead to environmental pollution and health risk to human. Therefore, biological products such as fungal-based products can be considered as an excellent alternative to overcome this issue. This study aimed to characterize and evaluate the potential of Trichoderma spp. as a biological control agent, biodecomposer and plant growth promoting fungi (PGPF). Firstly, three selected Trichoderma isolates from stem and leaves sample in Kelantan river were identified by molecular approach via Polymerase Chain Reaction (PCR) and morphological characterization such as colony character, growth rate and microscopic features. The characteristics of biological control capabilities were tested by dual culture assay. Next, Trichoderma spp. was evaluated as biodecomposer agent by assessing enzymatic degradation of plant cell wall components (lignin, cellulose, starch, xylan and pectin). The capabilities of Trichoderma spp. to degrade compost also were evaluated by monitoring the biological changes in temperature, pH and electrical conductivity of compost. The macronutrients and micronutrients concentration of treated compost were analyzed within 28 days. Finally, the (PGPF) was tested by the effect of seed treatment and soil application with the three potential Trichoderma spp. on Brasicca rapa subsp. chinensis (Pak Choi) growth. The selected Trichoderma spp. shows similar colony characters (compact, cottonish and formed concentric ring) but slightly different in color appearances. However, molecular identification revealed that the selected Trichoderma spp. were Trichoderma hamatum, Trichoderma harzianum and Trichoderma atroviride. The outcome of dual culture assay revealed that T. harzianum caused maximum inhibition (56.09 % - 85.71%) followed by T. hamatum (48.78% - 83.48%) and T. atroviride (48.78% - 83.48%) against several plant pathogens. In plate assay, all three Trichoderma isolates displayed highest cellulase, amylase, xylanase and pectinase activity based on the zone diameter of colour change at 9 cm. Moreover, moderate ligninase activities in the range of 1.4 cm to 2.59 cm of brown pigment diameter were displayed. Electrical conductivity was found to be 6083 ppm for T.hamatum-treated compost (compost A), 5843 ppm for T. atroviride-treated compost (compost C), 5702 ppm for T.harzianum-treated compost (compost B) and 4663 ppm for the control. The highest phosphorus (P) and sodium (Na) were obtained for compost A (402 ppm, 55.5 ppm) compared to the control (301 ppm, 38.3 ppm) whereas nitrate (NO3-) was found to be the highest in compost C at 1005 ppm compared to control (370ppm). Soil application with T. harzianum had been shown to significantly promote Pak Choy growth by increasing plant height (9.86 cm), root length (16.74 cm), root mass (73 x10-3), leaf area (21.5 cm2 ) and chloropyll content (38.34), whilst the control recorded at 3.8 cm, 9.54 cm,7.5 x10-3,4.87 cm2 and 33.1 respectively. The results in this study revealed that T.hamatum, T. harzianum and T. atroviride had high potential as a biological control towards several plant pathogens. Besides, they also act as a biodecomposer agent and could produce compost with enriched nutrient at the end of the composting period. In conclusion, the findings in this study could contribute potential future research directions on studies related to Trichoderma spp. and their biological functions towards sustainable agriculture. Trichoderma hamatum Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Trichoderma Hamatum Trichoderma Harzianum Trichoderma Atroviride Thesis |
| spellingShingle | 2021_Characterization and Biological Functions of Trichoderma hamatum, T. harzianum and T. atroviride As A Biological Control agent, Biodecomposer and Plant Growth Promoter |
| state | Terengganu |
| subject | Trichoderma hamatum Dissertations, Academic |
| summary | Trichoderma spp. have been widely used in agricultural application due to its well known biological control mechanism. The usage of Trichoderma-based products attracts the attention of researchers to discover more on other potential benefits of Trichoderma spp. mainly in issues concerning the food system including the use of excessive pesticide and chemical fertilizer. Besides, improper management of food wastes is also a rising problem recently which has lead to environmental pollution and health risk to human. Therefore, biological products such as fungal-based products can be considered as an excellent alternative to overcome this issue. This study aimed to characterize and evaluate the potential of Trichoderma spp. as a biological control agent, biodecomposer and plant growth promoting fungi (PGPF). Firstly, three selected Trichoderma isolates from stem and leaves sample in Kelantan river were identified by molecular approach via Polymerase Chain Reaction (PCR) and morphological characterization such as colony character, growth rate and microscopic features. The characteristics of biological control capabilities were tested by dual culture assay. Next, Trichoderma spp. was evaluated as biodecomposer agent by assessing enzymatic degradation of plant cell wall components (lignin, cellulose, starch, xylan and pectin). The capabilities of Trichoderma spp. to degrade compost also were evaluated by monitoring the biological changes in temperature, pH and electrical conductivity of compost. The macronutrients and micronutrients concentration of treated compost were analyzed within 28 days. Finally, the (PGPF) was tested by the effect of seed treatment and soil application with the three potential Trichoderma spp. on Brasicca rapa subsp. chinensis (Pak Choi) growth. The selected Trichoderma spp. shows similar colony characters (compact, cottonish and formed concentric ring) but slightly different in color appearances. However, molecular identification revealed that the selected Trichoderma spp. were Trichoderma hamatum, Trichoderma harzianum and Trichoderma atroviride. The outcome of dual culture assay revealed that T. harzianum caused maximum inhibition (56.09 % - 85.71%) followed by T. hamatum (48.78% - 83.48%) and T. atroviride (48.78% - 83.48%) against several plant pathogens. In plate assay, all three Trichoderma isolates displayed highest cellulase, amylase, xylanase and pectinase activity based on the zone diameter of colour change at 9 cm. Moreover, moderate ligninase activities in the range of 1.4 cm to 2.59 cm of brown pigment diameter were displayed. Electrical conductivity was found to be 6083 ppm for T.hamatum-treated compost (compost A), 5843 ppm for T. atroviride-treated compost (compost C), 5702 ppm for T.harzianum-treated compost (compost B) and 4663 ppm for the control. The highest phosphorus (P) and sodium (Na) were obtained for compost A (402 ppm, 55.5 ppm) compared to the control (301 ppm, 38.3 ppm) whereas nitrate (NO3-) was found to be the highest in compost C at 1005 ppm compared to control (370ppm). Soil application with T. harzianum had been shown to significantly promote Pak Choy growth by increasing plant height (9.86 cm), root length (16.74 cm), root mass (73 x10-3), leaf area (21.5 cm2 ) and chloropyll content (38.34), whilst the control recorded at 3.8 cm, 9.54 cm,7.5 x10-3,4.87 cm2 and 33.1 respectively. The results in this study revealed that T.hamatum, T. harzianum and T. atroviride had high potential as a biological control towards several plant pathogens. Besides, they also act as a biodecomposer agent and could produce compost with enriched nutrient at the end of the composting period. In conclusion, the findings in this study could contribute potential future research directions on studies related to Trichoderma spp. and their biological functions towards sustainable agriculture. |
| title | 2021_Characterization and Biological Functions of Trichoderma hamatum, T. harzianum and T. atroviride As A Biological Control agent, Biodecomposer and Plant Growth Promoter |
| title_full | 2021_Characterization and Biological Functions of Trichoderma hamatum, T. harzianum and T. atroviride As A Biological Control agent, Biodecomposer and Plant Growth Promoter |
| title_fullStr | 2021_Characterization and Biological Functions of Trichoderma hamatum, T. harzianum and T. atroviride As A Biological Control agent, Biodecomposer and Plant Growth Promoter |
| title_full_unstemmed | 2021_Characterization and Biological Functions of Trichoderma hamatum, T. harzianum and T. atroviride As A Biological Control agent, Biodecomposer and Plant Growth Promoter |
| title_short | 2021_Characterization and Biological Functions of Trichoderma hamatum, T. harzianum and T. atroviride As A Biological Control agent, Biodecomposer and Plant Growth Promoter |
| title_sort | 2021_characterization and biological functions of trichoderma hamatum, t. harzianum and t. atroviride as a biological control agent, biodecomposer and plant growth promoter |