2023_Allelopathic Potential of Turnera subulata Leaf on Selected Bioassay Species
| Format: | General Document |
|---|
| _version_ | 1860798016888242176 |
|---|---|
| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2023-05-24 |
| format | General Document |
| id | 15487 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih |
| originalfilename | ALLELOPATHIC POTENTIAL OF Turnera subulata LEAF ON SELECTED BIOASSAY SPECIES (MASTER_2023).pdf |
| person | Norhafiza Binti Yaakob |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15487 |
| sourcemedia | Server storage Scanned document |
| spelling | 15487 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15487 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access UNIVERSITI SULTAN ZAINAL ABIDIN SAMBox 2.4.24; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 2023-05-24 198 ALLELOPATHIC POTENTIAL OF Turnera subulata LEAF ON SELECTED BIOASSAY SPECIES (MASTER_2023).pdf Norhafiza Binti Yaakob Copyright©PWB2025 The repeated use of herbicides contributed to the increasing number of cases of herbicide resistance, environmental pollution, and human health problems. Turnera subulata Sm. has the potential in controlling insect pest populations in the rice field and has medicinal value. However, there is limited study has been conducted related to the allelopathic activity of T. subulata. This study aims to determine the allelopathic potential of T. subulata leaf, to evaluate the effect of T. subulata leaf litter and leaf extract on the growth and the changes in soil nutrients content of bioassay species, and to identify potential allelochemicals compounds from T. subulata leaf. T. subulata leaves (yellow alder) were used as the donor plant in this research. Whilst, the selected bioassay species tested in this research were L. sativa (lettuce), B. chinensis (mustard), Oryza sativa var sativa (weedy rice), and T. aestivum (wheat). The sandwich and dish pack methods were carried out to evaluate the allelopathic potential of T. subulata. Further, the growth response and changes in soil nutrients level of bioassay species for soil amended with T. subulata leaf litter and leaf litter extract in different concentrations were analyzed. The potential allelochemicals from the leaf of T. subulata were identified using High-Performance Thin Layer Chromatography (HPTLC) and Gas Chromatography- Mass Spectrometry (GCMS) analysis. The result shows that the radicle length of weedy rice was inhibited by 79.05% compared to control at 50 mg of T. subulata leaf in the sandwich method. In the dish pack bioassay, the radicle length of lettuce was observed with the highest inhibitory effect (72.41%) compared to control at 41 mm from donor source well. The soil amended with leaf litter and leaf extract of T. subulata showed an inhibitory effect, however, leaf litter had more inhibitory effects. The inhibition in growth was concentration-dependent and weedy rice experienced the highest reduction among all test plants. Partial inhibition was observed in the chlorophyll content and leaf area of weedy rice as the concentration of the soil amendment or the extract increased. In this research, rutin and naringin were identified by using HPTLC analysis. Meanwhile, 27 chemical compounds from T. subulata leaf had been identified from GC-MS analysis. The results suggested that T. subulata leaf has allelopathic potential on the growth of selected bioassay species. Leaf litter and leaf extract of T. subulata significantly inhibited (p< 0.05) the growth and physiological parameters of bioassay species and caused significant changes in soil nutrientss levels. HPTLC and GC MS analysis revealed the presence of flavonoid and non-protein amino acid compounds respectively from T. subulata leaf. Exploring T. subulata allelopathy could be a promising alternative in achieving better weed management for sustainable agriculture. 2023_Allelopathic Potential of Turnera subulata Leaf on Selected Bioassay Species Allelopathy Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic untuk semua tesis.. Terima kasih Turnera Subulata Allelopathic Potential Leaf Extract Thesis |
| spellingShingle | 2023_Allelopathic Potential of Turnera subulata Leaf on Selected Bioassay Species |
| state | Terengganu |
| subject | Allelopathy Dissertations, Academic |
| summary | The repeated use of herbicides contributed to the increasing number of cases of herbicide resistance, environmental pollution, and human health problems. Turnera subulata Sm. has the potential in controlling insect pest populations in the rice field and has medicinal value. However, there is limited study has been conducted related to the allelopathic activity of T. subulata. This study aims to determine the allelopathic potential of T. subulata leaf, to evaluate the effect of T. subulata leaf litter and leaf extract on the growth and the changes in soil nutrients content of bioassay species, and to identify potential allelochemicals compounds from T. subulata leaf. T. subulata leaves (yellow alder) were used as the donor plant in this research. Whilst, the selected bioassay species tested in this research were L. sativa (lettuce), B. chinensis (mustard), Oryza sativa var sativa (weedy rice), and T. aestivum (wheat). The sandwich and dish pack methods were carried out to evaluate the allelopathic potential of T. subulata. Further, the growth response and changes in soil nutrients level of bioassay species for soil amended with T. subulata leaf litter and leaf litter extract in different concentrations were analyzed. The potential allelochemicals from the leaf of T. subulata were identified using High-Performance Thin Layer Chromatography (HPTLC) and Gas Chromatography- Mass Spectrometry (GCMS) analysis. The result shows that the radicle length of weedy rice was inhibited by 79.05% compared to control at 50 mg of T. subulata leaf in the sandwich method. In the dish pack bioassay, the radicle length of lettuce was observed with the highest inhibitory effect (72.41%) compared to control at 41 mm from donor source well. The soil amended with leaf litter and leaf extract of T. subulata showed an inhibitory effect, however, leaf litter had more inhibitory effects. The inhibition in growth was concentration-dependent and weedy rice experienced the highest reduction among all test plants. Partial inhibition was observed in the chlorophyll content and leaf area of weedy rice as the concentration of the soil amendment or the extract increased. In this research, rutin and naringin were identified by using HPTLC analysis. Meanwhile, 27 chemical compounds from T. subulata leaf had been identified from GC-MS analysis. The results suggested that T. subulata leaf has allelopathic potential on the growth of selected bioassay species. Leaf litter and leaf extract of T. subulata significantly inhibited (p< 0.05) the growth and physiological parameters of bioassay species and caused significant changes in soil nutrientss levels. HPTLC and GC MS analysis revealed the presence of flavonoid and non-protein amino acid compounds respectively from T. subulata leaf. Exploring T. subulata allelopathy could be a promising alternative in achieving better weed management for sustainable agriculture. |
| title | 2023_Allelopathic Potential of Turnera subulata Leaf on Selected Bioassay Species |
| title_full | 2023_Allelopathic Potential of Turnera subulata Leaf on Selected Bioassay Species |
| title_fullStr | 2023_Allelopathic Potential of Turnera subulata Leaf on Selected Bioassay Species |
| title_full_unstemmed | 2023_Allelopathic Potential of Turnera subulata Leaf on Selected Bioassay Species |
| title_short | 2023_Allelopathic Potential of Turnera subulata Leaf on Selected Bioassay Species |
| title_sort | 2023_allelopathic potential of turnera subulata leaf on selected bioassay species |