2015_In Vitro Antioxidant Activity and in Vivo Antigastric Ulcer Activity of Tepal and Skin Extracts of Musa Paradisiaca in Mice
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2015-11-01 |
| format | General Document |
| id | 15480 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | 15480_d1b9d6dad23de31.pdf |
| person | Bashir Ado Ahmad |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15480 |
| sourcemedia | Server storage Scanned document |
| spelling | 15480 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15480 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 170 15480_d1b9d6dad23de31.pdf 2015_In Vitro Antioxidant Activity and in Vivo Antigastric Ulcer Activity of Tepal and Skin Extracts of Musa Paradisiaca in Mice Copyright©PWB2025 Bashir Ado Ahmad 2015-11-01 Antioxidants—Pharmacology The current use of ulcer drugs is limited to its side effects and potentiality of relapse. This study is aimed to explore the antioxidant activity of banana tepal methanol (TPM), ethanol (TE), aqueous (TA), skin (SKM) and flesh methanol (FM) extracts and ascertain the antiulcer potentials of the TPM and SKM. The banana parts were processed and extracted by simple maceration. Some phytochemicals were screened. Antioxidant activity was assessed using 2,2-diphenyl-1-picryl hydrazine (DPPH) and nitric oxide (NO) radicals scavenging activities. Total phenolic content (TPC) was measured. Twenty-five albino mice were fasted for 24 hours and then randomly grouped into five, comprising five mice each. The control group received distilled water. TPM, SKM and cimetidine (CIM) groups were pretreated with 100mg/kg tepal, skin and cimetidine for seven days respectively. On day seven, a single dose of indomethacin (48mg/kg) was administered, in this case including the induced group (IND+PYL). All the animals were fasted overnight. On the 8th day, the pylorus was ligated. The animals were sacrificed, and the removed stomachs were prepared for the evaluation of ulcer index and gastric wall mucus. pH and volume were measured from the gastric juice. The results revealed some phytochemicals like phenols, flavonoids etc., in the extracts. TPM had the highest DPPH radical scavenging activity (IC50=22.5 µg/mL) while FM has the least (no IC50). Maximum NO inhibition was seen in TA (41.05%) and still FM showed the least (22.34%). TPM had the highest TPC (8000 µg GAE/g extract) than all other extracts. TPM and SKM prevented the induced ulcer by 68.80±20.53% and 43.22±14.82% respectively. Significant rise (p<0.05) in gastric juice pH (3.79±0.24) was noticed in TPM group. However, the decrease in gastric juice volume and increased gastric wall mucus by TPM and SKM were not statistically significant (P>0.05). The results showed that TPM and SKM were able to prevent IND+PYL induced ulcer; possibly by strengthening the gastric mucosa, decreasing the gastric juice acidity and antioxidative mechanism. Further investigations are needed to validate the use of these parts for ulcer treatment. Antioxidants Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Plant Extracts Gastrointestinal System Thesis |
| spellingShingle | 2015_In Vitro Antioxidant Activity and in Vivo Antigastric Ulcer Activity of Tepal and Skin Extracts of Musa Paradisiaca in Mice |
| state | Terengganu |
| subject | Antioxidants—Pharmacology Dissertations, Academic |
| summary | The current use of ulcer drugs is limited to its side effects and potentiality of relapse. This study is aimed to explore the antioxidant activity of banana tepal methanol (TPM), ethanol (TE), aqueous (TA), skin (SKM) and flesh methanol (FM) extracts and ascertain the antiulcer potentials of the TPM and SKM. The banana parts were processed and extracted by simple maceration. Some phytochemicals were screened. Antioxidant activity was assessed using 2,2-diphenyl-1-picryl hydrazine (DPPH) and nitric oxide (NO) radicals scavenging activities. Total phenolic content (TPC) was measured. Twenty-five albino mice were fasted for 24 hours and then randomly grouped into five, comprising five mice each. The control group received distilled water. TPM, SKM and cimetidine (CIM) groups were pretreated with 100mg/kg tepal, skin and cimetidine for seven days respectively. On day seven, a single dose of indomethacin (48mg/kg) was administered, in this case including the induced group (IND+PYL). All the animals were fasted overnight. On the 8th day, the pylorus was ligated. The animals were sacrificed, and the removed stomachs were prepared for the evaluation of ulcer index and gastric wall mucus. pH and volume were measured from the gastric juice. The results revealed some phytochemicals like phenols, flavonoids etc., in the extracts. TPM had the highest DPPH radical scavenging activity (IC50=22.5 µg/mL) while FM has the least (no IC50). Maximum NO inhibition was seen in TA (41.05%) and still FM showed the least (22.34%). TPM had the highest TPC (8000 µg GAE/g extract) than all other extracts. TPM and SKM prevented the induced ulcer by 68.80±20.53% and 43.22±14.82% respectively. Significant rise (p<0.05) in gastric juice pH (3.79±0.24) was noticed in TPM group. However, the decrease in gastric juice volume and increased gastric wall mucus by TPM and SKM were not statistically significant (P>0.05). The results showed that TPM and SKM were able to prevent IND+PYL induced ulcer; possibly by strengthening the gastric mucosa, decreasing the gastric juice acidity and antioxidative mechanism. Further investigations are needed to validate the use of these parts for ulcer treatment. |
| title | 2015_In Vitro Antioxidant Activity and in Vivo Antigastric Ulcer Activity of Tepal and Skin Extracts of Musa Paradisiaca in Mice |
| title_full | 2015_In Vitro Antioxidant Activity and in Vivo Antigastric Ulcer Activity of Tepal and Skin Extracts of Musa Paradisiaca in Mice |
| title_fullStr | 2015_In Vitro Antioxidant Activity and in Vivo Antigastric Ulcer Activity of Tepal and Skin Extracts of Musa Paradisiaca in Mice |
| title_full_unstemmed | 2015_In Vitro Antioxidant Activity and in Vivo Antigastric Ulcer Activity of Tepal and Skin Extracts of Musa Paradisiaca in Mice |
| title_short | 2015_In Vitro Antioxidant Activity and in Vivo Antigastric Ulcer Activity of Tepal and Skin Extracts of Musa Paradisiaca in Mice |
| title_sort | 2015_in vitro antioxidant activity and in vivo antigastric ulcer activity of tepal and skin extracts of musa paradisiaca in mice |