2015_In Vitro Investigation of Cytotoxicity and Apoptosis Induction of Hepcidin (TH1-5) On Human Breast Cancer Cell Line MCF-7
| Format: | General Document |
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| _version_ | 1860798014698815488 |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2015-12-20 |
| format | General Document |
| id | 15478 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | IN VITRO INVESTIGATION OF CYTOTOXICITY AND APOPTOSIS INDUCTION OF HEPCIDIN (TH1-5) ON HUMAN BREAST CANCER CELL LINE MCF-7 (MASTER_2015).pdf |
| person | Mohammed Al-Kassim Hassan |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15478 |
| sourcemedia | Server storage Scanned document |
| spelling | 15478 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15478 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 116 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) IN VITRO INVESTIGATION OF CYTOTOXICITY AND APOPTOSIS INDUCTION OF HEPCIDIN (TH1-5) ON HUMAN BREAST CANCER CELL LINE MCF-7 (MASTER_2015).pdf 2015-12-20 2015_In Vitro Investigation of Cytotoxicity and Apoptosis Induction of Hepcidin (TH1-5) On Human Breast Cancer Cell Line MCF-7 Copyright©PWB2025 Mohammed Al-Kassim Hassan Cancer cell apoptosis—Induction Hepcidins are cysteine-rich antimicrobial peptides that have been identified from various fish species. Hepcidin (TH1-5) have been isolated from freshwater tilapia fish Oreochromis mossambicus. Synthetic isoform of this peptide was previously reported for its antimicrobial, anti-inflammatory, wound-healing and cytotoxic functions. Its cytotoxic activities have been studied against human cancer cell lines including cervical, hepatocellular and fibrosarcoma cells. This study aims to investigate the potential cytotoxicity of the hepcidin (TH1-5) synthetic peptide on human breast cancer cell line MCF-7. Cell viability was examined using the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay. Mode of cell death was determined via the acridine orange-propidium iodide double staining technique as well as annexin V FITC apoptotic assay. Apoptosis mechanisms through caspase activation from hepcidin (TH1-5) induction was also analysed. Results showed the peptide to exert cytotoxic effect with a half maximal inhibitory concentration (IC50) of 20 µg/mL but less cytotoxic to normal mouse embryonic fibroblast cells NIH/3T3. Fluorescence microscopy for mode of cell death revealed majority of the cell underwent apoptotic cell death after 24, 48 and 72 hours of treatment. The peptide also demonstrated increased caspase (caspase-3/7 and caspase-9) enzymatic activity and activate the mitochondrial intrinsic pathway of apoptosis. These results suggest that hepcidin (TH1 5) possess cytotoxic properties and induce apoptosis via the intrinsic pathway. In addition to its role in iron homeostasis, hepcidin can be developed as a potential chemotherapeutic candidate for breast cancer therapy. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... In Vitro Cytotoxicity Breast Cancer Treatment MCF-7 Breast Cancer Cells Thesis |
| spellingShingle | 2015_In Vitro Investigation of Cytotoxicity and Apoptosis Induction of Hepcidin (TH1-5) On Human Breast Cancer Cell Line MCF-7 |
| state | Terengganu |
| subject | Cancer cell apoptosis—Induction Dissertations, Academic |
| summary | Hepcidins are cysteine-rich antimicrobial peptides that have been identified from various fish species. Hepcidin (TH1-5) have been isolated from freshwater tilapia fish Oreochromis mossambicus. Synthetic isoform of this peptide was previously reported for its antimicrobial, anti-inflammatory, wound-healing and cytotoxic functions. Its cytotoxic activities have been studied against human cancer cell lines including cervical, hepatocellular and fibrosarcoma cells. This study aims to investigate the potential cytotoxicity of the hepcidin (TH1-5) synthetic peptide on human breast cancer cell line MCF-7. Cell viability was examined using the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay. Mode of cell death was determined via the acridine orange-propidium iodide double staining technique as well as annexin V FITC apoptotic assay. Apoptosis mechanisms through caspase activation from hepcidin (TH1-5) induction was also analysed. Results showed the peptide to exert cytotoxic effect with a half maximal inhibitory concentration (IC50) of 20 µg/mL but less cytotoxic to normal mouse embryonic fibroblast cells NIH/3T3. Fluorescence microscopy for mode of cell death revealed majority of the cell underwent apoptotic cell death after 24, 48 and 72 hours of treatment. The peptide also demonstrated increased caspase (caspase-3/7 and caspase-9) enzymatic activity and activate the mitochondrial intrinsic pathway of apoptosis. These results suggest that hepcidin (TH1 5) possess cytotoxic properties and induce apoptosis via the intrinsic pathway. In addition to its role in iron homeostasis, hepcidin can be developed as a potential chemotherapeutic candidate for breast cancer therapy. |
| title | 2015_In Vitro Investigation of Cytotoxicity and Apoptosis Induction of Hepcidin (TH1-5) On Human Breast Cancer Cell Line MCF-7 |
| title_full | 2015_In Vitro Investigation of Cytotoxicity and Apoptosis Induction of Hepcidin (TH1-5) On Human Breast Cancer Cell Line MCF-7 |
| title_fullStr | 2015_In Vitro Investigation of Cytotoxicity and Apoptosis Induction of Hepcidin (TH1-5) On Human Breast Cancer Cell Line MCF-7 |
| title_full_unstemmed | 2015_In Vitro Investigation of Cytotoxicity and Apoptosis Induction of Hepcidin (TH1-5) On Human Breast Cancer Cell Line MCF-7 |
| title_short | 2015_In Vitro Investigation of Cytotoxicity and Apoptosis Induction of Hepcidin (TH1-5) On Human Breast Cancer Cell Line MCF-7 |
| title_sort | 2015_in vitro investigation of cytotoxicity and apoptosis induction of hepcidin (th1-5) on human breast cancer cell line mcf-7 |