2020_In Vitro Antibacterial Properties of Etlingera Elatior Flower Extracts Against Acne-Inducing Bacteria
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2020-06-22 |
| format | General Document |
| id | 15477 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | 15477_870a207c8428965.pdf |
| person | Nurul Saidah Binti Din |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15477 |
| sourcemedia | Server storage Scanned document |
| spelling | 15477 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15477 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 144 15477_870a207c8428965.pdf 2020_In Vitro Antibacterial Properties of Etlingera Elatior Flower Extracts Against Acne-Inducing Bacteria Copyright©PWB2025 Nurul Saidah Binti Din 2020-06-22 Etlingera elatior—Therapeutic use Acne is one of the most commonly encountered dermatological problems and affects approximately 80% of adolescents. Multiple factors are associated with the pathogenesis of acne and bacterial colonisation has been identified as one of the causative factors involved. Propionibacterium acnes and Staphylococcus aureus are known to be among the main pathogens of acne. Treatment with currently available antibacterial agents may lead to unfavourable side-effects and the emergence of resistant strains. Etlingera elatior flower or ‘bunga kantan' is a well-known medicinal plant in Malaysia with various pharmaceutical properties including antibacterial. However, the antibacterial properties of E. elatior flower extracts against these bacteria are inadequately reported. Thus, this study aimed to investigate the antibacterial properties of the flower extracts against the two acne-inducing bacteria, P. acnes and S. aureus. E. elatior flowers extracts were obtained using three solvents with different polarities known as n-hexane (non-polar), dichloromethane (semi-polar) and ethanol (polar). Their antibacterial properties were evaluated by disk diffusion and broth dilution assays by determining the inhibition zone diameter, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Total Phenolic Acid (TPC) and Total Flavonoid Content (TFC) were estimated using Folin-Ciocalteu and aluminium chloride colorimetric assay respectively. Morphological changes of the treated bacteria were studied using a scanning electron microscope (SEM). All of the extracts were found to have antibacterial properties against the bacteria. The dichloromethane E. elatior flowers extract showed the highest antibacterial properties against P. acnes whereby at the concentration of 25 mg/mL, it has demonstrated the widest inhibition zone (11.39 ± 0.45 mm) and the lowest MIC (6.25 mg/mL) and MBC (12.5 mg/mL). The ethanolic extract of the flowers has shown the highest antibacterial properties against S. aureus whereby at the concentration of 50 mg/mL, the inhibition zone was 6.21 ± 0.25 mm and the MIC and MBC were both 12.5 mg/mL. The ethanolic E. elatior flowers extract also demonstrated the highest phenolic acid and flavonoid contents; 966.304 ± 114.08 mg GAE/g and 796.33 ± 65.78 mg QE/g, respectively. This was followed by the dichloromethane and n-hexane E. elatior flowers extracts. Significant morphological changes of the treated bacteria were observed. This result suggests that E. elatior flowers extracts exhibited antibacterial properties against P. acnes and S. aureus and could potentially be used as an alternative treatment against acne. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Antibacterial Activity Etlingera Elatior Acne Treatment Thesis |
| spellingShingle | 2020_In Vitro Antibacterial Properties of Etlingera Elatior Flower Extracts Against Acne-Inducing Bacteria |
| state | Terengganu |
| subject | Etlingera elatior—Therapeutic use Dissertations, Academic |
| summary | Acne is one of the most commonly encountered dermatological problems and affects approximately 80% of adolescents. Multiple factors are associated with the pathogenesis of acne and bacterial colonisation has been identified as one of the causative factors involved. Propionibacterium acnes and Staphylococcus aureus are known to be among the main pathogens of acne. Treatment with currently available antibacterial agents may lead to unfavourable side-effects and the emergence of resistant strains. Etlingera elatior flower or ‘bunga kantan' is a well-known medicinal plant in Malaysia with various pharmaceutical properties including antibacterial. However, the antibacterial properties of E. elatior flower extracts against these bacteria are inadequately reported. Thus, this study aimed to investigate the antibacterial properties of the flower extracts against the two acne-inducing bacteria, P. acnes and S. aureus. E. elatior flowers extracts were obtained using three solvents with different polarities known as n-hexane (non-polar), dichloromethane (semi-polar) and ethanol (polar). Their antibacterial properties were evaluated by disk diffusion and broth dilution assays by determining the inhibition zone diameter, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Total Phenolic Acid (TPC) and Total Flavonoid Content (TFC) were estimated using Folin-Ciocalteu and aluminium chloride colorimetric assay respectively. Morphological changes of the treated bacteria were studied using a scanning electron microscope (SEM). All of the extracts were found to have antibacterial properties against the bacteria. The dichloromethane E. elatior flowers extract showed the highest antibacterial properties against P. acnes whereby at the concentration of 25 mg/mL, it has demonstrated the widest inhibition zone (11.39 ± 0.45 mm) and the lowest MIC (6.25 mg/mL) and MBC (12.5 mg/mL). The ethanolic extract of the flowers has shown the highest antibacterial properties against S. aureus whereby at the concentration of 50 mg/mL, the inhibition zone was 6.21 ± 0.25 mm and the MIC and MBC were both 12.5 mg/mL. The ethanolic E. elatior flowers extract also demonstrated the highest phenolic acid and flavonoid contents; 966.304 ± 114.08 mg GAE/g and 796.33 ± 65.78 mg QE/g, respectively. This was followed by the dichloromethane and n-hexane E. elatior flowers extracts. Significant morphological changes of the treated bacteria were observed. This result suggests that E. elatior flowers extracts exhibited antibacterial properties against P. acnes and S. aureus and could potentially be used as an alternative treatment against acne. |
| title | 2020_In Vitro Antibacterial Properties of Etlingera Elatior Flower Extracts Against Acne-Inducing Bacteria |
| title_full | 2020_In Vitro Antibacterial Properties of Etlingera Elatior Flower Extracts Against Acne-Inducing Bacteria |
| title_fullStr | 2020_In Vitro Antibacterial Properties of Etlingera Elatior Flower Extracts Against Acne-Inducing Bacteria |
| title_full_unstemmed | 2020_In Vitro Antibacterial Properties of Etlingera Elatior Flower Extracts Against Acne-Inducing Bacteria |
| title_short | 2020_In Vitro Antibacterial Properties of Etlingera Elatior Flower Extracts Against Acne-Inducing Bacteria |
| title_sort | 2020_in vitro antibacterial properties of etlingera elatior flower extracts against acne-inducing bacteria |