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1860798014298259456
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3
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Copyright©PWB2025
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Malaysia
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2015-12-13
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General Document
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15476
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UniSZA
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Sila masukkan subject wajib Dissertations, Academic. Terima kasih...
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INVITR~2.PDF
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Yahaya Najib Sani
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15476
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Server storage
Scanned document
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15476 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15476 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 116 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) INVITR~2.PDF 2015_ In Vitro Antibacterial, Anti-Inflammatory and Antidiabetic Study of Andrographis Paniculata (BURM. F) WALL. EX NEES Extracts and Isolation of Its Constituents 2015-12-13 Copyright©PWB2025 Andrographis paniculata—Therapeutic use Andrographis, botanically known as Andrographis paniculata (Burm. F) Wall. ex Nees is a South East Asian native plant belonging to the family Acanthaceae. The plant is locally known as ―Hempedu Bumi‖ in Malaysia, and it is recognised for its medicinal values and has been utilised for treating several diseases. The first part of the study involves investigating the effect of extracting solvents on percentage yield, phytochemical screening, total phenolic contents (TPC), total flavonoid contents (TFC) and antioxidant activity of A. paniculata extracts. Ethanol: water extract (E:W) at a ratio of 1:1 (v/v) showed the highest percentage yield compared to other extracts, while aqueous extract was a better solvent in extracting six group of chemicals (saponins, phenols, flavonoids, alkaloids, tannins and terpenoids) and recorded the highest TPC of 184.48 mg of GAE/g, followed by E:W, methanol and ethyl acetate (178.79, 58.78 and 9.37 mg GAE/g) respectively. Ethyl acetate extract recorded the highest TFC of 0.44 mg of QE/g, while methanol, E:W and aqueous extracts had similar average values for TFC (0.43 mg QE/g). However, E:W indicated a better antioxidant activity with an IC50 of 93.30 µg/mL while the other three extracts had activity below 50% inhibition rate. The second part of the study involves isolation and characterisation of compounds from A. paniculata. Proton and carbon nuclear magnetic resonance (1H and 13C NMR) of one of the isolated compound (APO3) indicated a semi–pure compound and its identity is suggested to be andrographolide. The next part of the study involved in vitro antibacterial study where methanol extract showed a better antibacterial activity against both S. epidermidis and E. coli compared to the other extracts. Additionally, in vitro anti-inflammatory activity indicated that ethyl acetate extract was a better nitric oxide (NO) inhibitor compared with other extracts while the isolated compounds (APO3 and APO4) demonstrated significant inhibition of NO, indicating a promising anti-inflammatory activity. Finally, α glucosidase enzyme inhibition study by A. paniculata extracts indicated the potential antidiabetic activity of ethyl acetate extract, which was the best. In conclusion, this study shows that although A. paniculata possesses multiple biological activities, the choice of solvent to be used is an important consideration to obtain maximum pharmacological effect. Yahaya Najib Sani Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Antibacterial Activity Andrographis Paniculata Medicinal Plant Extracts Thesis
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| spellingShingle |
2015_ In Vitro Antibacterial, Anti-Inflammatory and Antidiabetic Study of Andrographis Paniculata (BURM. F) WALL. EX NEES Extracts and Isolation of Its Constituents
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| state |
Terengganu
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| subject |
Andrographis paniculata—Therapeutic use
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| summary |
Andrographis, botanically known as Andrographis paniculata (Burm. F) Wall. ex Nees is a South East Asian native plant belonging to the family Acanthaceae. The plant is locally known as ―Hempedu Bumi‖ in Malaysia, and it is recognised for its medicinal values and has been utilised for treating several diseases. The first part of the study involves investigating the effect of extracting solvents on percentage yield, phytochemical screening, total phenolic contents (TPC), total flavonoid contents (TFC) and antioxidant activity of A. paniculata extracts. Ethanol: water extract (E:W) at a ratio of 1:1 (v/v) showed the highest percentage yield compared to other extracts, while aqueous extract was a better solvent in extracting six group of chemicals (saponins, phenols, flavonoids, alkaloids, tannins and terpenoids) and recorded the highest TPC of 184.48 mg of GAE/g, followed by E:W, methanol and ethyl acetate (178.79, 58.78 and 9.37 mg GAE/g) respectively. Ethyl acetate extract recorded the highest TFC of 0.44 mg of QE/g, while methanol, E:W and aqueous extracts had similar average values for TFC (0.43 mg QE/g). However, E:W indicated a better antioxidant activity with an IC50 of 93.30 µg/mL while the other three extracts had activity below 50% inhibition rate. The second part of the study involves isolation and characterisation of compounds from A. paniculata. Proton and carbon nuclear magnetic resonance (1H and 13C NMR) of one of the isolated compound (APO3) indicated a semi–pure compound and its identity is suggested to be andrographolide. The next part of the study involved in vitro antibacterial study where methanol extract showed a better antibacterial activity against both S. epidermidis and E. coli compared to the other extracts. Additionally, in vitro anti-inflammatory activity indicated that ethyl acetate extract was a better nitric oxide (NO) inhibitor compared with other extracts while the isolated compounds (APO3 and APO4) demonstrated significant inhibition of NO, indicating a promising anti-inflammatory activity. Finally, α glucosidase enzyme inhibition study by A. paniculata extracts indicated the potential antidiabetic activity of ethyl acetate extract, which was the best. In conclusion, this study shows that although A. paniculata possesses multiple biological activities, the choice of solvent to be used is an important consideration to obtain maximum pharmacological effect.
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| title |
2015_ In Vitro Antibacterial, Anti-Inflammatory and Antidiabetic Study of Andrographis Paniculata (BURM. F) WALL. EX NEES Extracts and Isolation of Its Constituents
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| title_full |
2015_ In Vitro Antibacterial, Anti-Inflammatory and Antidiabetic Study of Andrographis Paniculata (BURM. F) WALL. EX NEES Extracts and Isolation of Its Constituents
|
| title_fullStr |
2015_ In Vitro Antibacterial, Anti-Inflammatory and Antidiabetic Study of Andrographis Paniculata (BURM. F) WALL. EX NEES Extracts and Isolation of Its Constituents
|
| title_full_unstemmed |
2015_ In Vitro Antibacterial, Anti-Inflammatory and Antidiabetic Study of Andrographis Paniculata (BURM. F) WALL. EX NEES Extracts and Isolation of Its Constituents
|
| title_short |
2015_ In Vitro Antibacterial, Anti-Inflammatory and Antidiabetic Study of Andrographis Paniculata (BURM. F) WALL. EX NEES Extracts and Isolation of Its Constituents
|
| title_sort |
2015_ in vitro antibacterial, anti-inflammatory and antidiabetic study of andrographis paniculata (burm. f) wall. ex nees extracts and isolation of its constituents
|