2023_Free Radical Scavenging Capacity and Anti-Inflammatory Effects of Abelmoschus Esculentus (L.) in Tumour Necrosis Factor-Alpha (TNF-a)-Induced SW982 Cell Line
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| date | 2023-06-22 |
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| spelling | 15468 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15468 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.4.24; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 246 FREERA~1.PDF 2023_Free Radical Scavenging Capacity and Anti-Inflammatory Effects of Abelmoschus Esculentus (L.) in Tumour Necrosis Factor-Alpha (TNF-a)-Induced SW982 Cell Line 2023-06-22 Abelmoschus esculentus (L.) Moench (A. esculentus), often known as okra, is a commercial vegetable crop grown worldwide and utilised as a medicinal herb traditionally. Based on previous research, A. esculentus has antioxidant and anti inflammatory properties. However, there is a scarcity finding of anti-inflammatory effects of A. esculentus on chronic inflammatory condition, potentially leading to arthritis development. Thus, this study was aimed to evaluate the antioxidant activities and anti-inflammatory effects of A. esculentus extracts in a cell line-induced model. The fruit of A. esculentus was collected and extracted sequentially producing hexane (HE), chloroform (CE), methanol (ME) and aqueous (AE) extracts. The qualitative preliminary phytochemical screening, the total phenolic content (TPC) and total flavonoid contents (TFC) were performed. The anti-oxidant and the free scavenging activity were performed using 1,1-diphenyl-2-picrylhidrazyl (DPPH) and reducing power assays. The embryotoxicity and cytotoxicity assay of extracts were determined using zebrafish embryos and synovial sarcoma cell line (SW982), respectively. For the inflammatory-induced model, SW982 cell line was treated with 10 ng/mL of tumour necrosis factor-alpha (TNF-α) before being treated with different concentration of extracts. Methotrexate was used as a positive control for all the assays. The anti inflammatory properties of A. esculentus were evaluated through intracellular calcium ion [(Ca2+)i] and nitric oxide (NO) assays. Phytochemical screening of A. esculentus showed the presence of flavonoids, tannins, phenols, saponins, anthraquinones, alkaloids, and reducing sugars. The CE and HE showed the highest TPC and TFC, respectively. The ME was presented with the highest free scavenging activity and reducing power. The HE exhibited a cytotoxic effect on SW982 cell lines at the half maximal inhibitory concentration (IC50) values of 907.75 ± 6.44 µg/mL, 600.54 ± 5.64 µg/mL and 449.55 ± 2.62 µg/mL at 24-, 48- and 72-hours treatment, respectively. No significant cytotoxic effect was observed on the SW982 cells treated with other A. esculentus extracts. The morphological changes including membrane blebbing, rounded cells as well as apoptotic bodies were observed in SW982 cell lines treated with HE at the IC50 values. The CE showed significant embryotoxicity effects with a median lethal concentration (LD50) of 236.07 µg/mL. In addition, morphological malformation, body curvature and pericardial oedema were presented in embryos treated with a high dose of HE and ME. The HE was presented with a significant IC50 value of 588.08 ± 6.70 µg/mL in TNF-α-induced SW982 cell lines at 48 hours of treatment. For anti-inflammatory activity, the ME and AE were observed to reduce in the concentration of (Ca2+)i, NO, nitrite and nitrate level significantly. From the findings, A. esculentus was postulated to be a potential source of natural antioxidants with vast phytochemical content. With the high IC50 and LD50, extract-dependent of A. esculentus was suggested to possess anti-inflammatory properties observed in the TNF α-induced SW982 cell line. This study provided a scientific basis of A. esculentus for further research in chronic inflammation diseases and arthritis. Copyright©PWB2025 Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Free radicals — Physiological effect Free Radical Scavenging Anti-Inflammatory Effects Abelmoschus Esculentus Thesis |
| spellingShingle | 2023_Free Radical Scavenging Capacity and Anti-Inflammatory Effects of Abelmoschus Esculentus (L.) in Tumour Necrosis Factor-Alpha (TNF-a)-Induced SW982 Cell Line |
| state | Terengganu |
| subject | Dissertations, Academic Free radicals — Physiological effect |
| summary | Abelmoschus esculentus (L.) Moench (A. esculentus), often known as okra, is a commercial vegetable crop grown worldwide and utilised as a medicinal herb traditionally. Based on previous research, A. esculentus has antioxidant and anti inflammatory properties. However, there is a scarcity finding of anti-inflammatory effects of A. esculentus on chronic inflammatory condition, potentially leading to arthritis development. Thus, this study was aimed to evaluate the antioxidant activities and anti-inflammatory effects of A. esculentus extracts in a cell line-induced model. The fruit of A. esculentus was collected and extracted sequentially producing hexane (HE), chloroform (CE), methanol (ME) and aqueous (AE) extracts. The qualitative preliminary phytochemical screening, the total phenolic content (TPC) and total flavonoid contents (TFC) were performed. The anti-oxidant and the free scavenging activity were performed using 1,1-diphenyl-2-picrylhidrazyl (DPPH) and reducing power assays. The embryotoxicity and cytotoxicity assay of extracts were determined using zebrafish embryos and synovial sarcoma cell line (SW982), respectively. For the inflammatory-induced model, SW982 cell line was treated with 10 ng/mL of tumour necrosis factor-alpha (TNF-α) before being treated with different concentration of extracts. Methotrexate was used as a positive control for all the assays. The anti inflammatory properties of A. esculentus were evaluated through intracellular calcium ion [(Ca2+)i] and nitric oxide (NO) assays. Phytochemical screening of A. esculentus showed the presence of flavonoids, tannins, phenols, saponins, anthraquinones, alkaloids, and reducing sugars. The CE and HE showed the highest TPC and TFC, respectively. The ME was presented with the highest free scavenging activity and reducing power. The HE exhibited a cytotoxic effect on SW982 cell lines at the half maximal inhibitory concentration (IC50) values of 907.75 ± 6.44 µg/mL, 600.54 ± 5.64 µg/mL and 449.55 ± 2.62 µg/mL at 24-, 48- and 72-hours treatment, respectively. No significant cytotoxic effect was observed on the SW982 cells treated with other A. esculentus extracts. The morphological changes including membrane blebbing, rounded cells as well as apoptotic bodies were observed in SW982 cell lines treated with HE at the IC50 values. The CE showed significant embryotoxicity effects with a median lethal concentration (LD50) of 236.07 µg/mL. In addition, morphological malformation, body curvature and pericardial oedema were presented in embryos treated with a high dose of HE and ME. The HE was presented with a significant IC50 value of 588.08 ± 6.70 µg/mL in TNF-α-induced SW982 cell lines at 48 hours of treatment. For anti-inflammatory activity, the ME and AE were observed to reduce in the concentration of (Ca2+)i, NO, nitrite and nitrate level significantly. From the findings, A. esculentus was postulated to be a potential source of natural antioxidants with vast phytochemical content. With the high IC50 and LD50, extract-dependent of A. esculentus was suggested to possess anti-inflammatory properties observed in the TNF α-induced SW982 cell line. This study provided a scientific basis of A. esculentus for further research in chronic inflammation diseases and arthritis. |
| title | 2023_Free Radical Scavenging Capacity and Anti-Inflammatory Effects of Abelmoschus Esculentus (L.) in Tumour Necrosis Factor-Alpha (TNF-a)-Induced SW982 Cell Line |
| title_full | 2023_Free Radical Scavenging Capacity and Anti-Inflammatory Effects of Abelmoschus Esculentus (L.) in Tumour Necrosis Factor-Alpha (TNF-a)-Induced SW982 Cell Line |
| title_fullStr | 2023_Free Radical Scavenging Capacity and Anti-Inflammatory Effects of Abelmoschus Esculentus (L.) in Tumour Necrosis Factor-Alpha (TNF-a)-Induced SW982 Cell Line |
| title_full_unstemmed | 2023_Free Radical Scavenging Capacity and Anti-Inflammatory Effects of Abelmoschus Esculentus (L.) in Tumour Necrosis Factor-Alpha (TNF-a)-Induced SW982 Cell Line |
| title_short | 2023_Free Radical Scavenging Capacity and Anti-Inflammatory Effects of Abelmoschus Esculentus (L.) in Tumour Necrosis Factor-Alpha (TNF-a)-Induced SW982 Cell Line |
| title_sort | 2023_free radical scavenging capacity and anti-inflammatory effects of abelmoschus esculentus (l.) in tumour necrosis factor-alpha (tnf-a)-induced sw982 cell line |