2020_Effects of Thymoquinone on Neurotoxic Rats Induced By 3, 4 Methylenedioxymethamphetamine (MDMA)
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2020-02-25 |
| format | General Document |
| id | 15408 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | EFFECTS OF THYMOQUINONE ON NEUROTOXIC RATS INDUCED BY 3, 4 METHYLENEDIOXYMETHAMPHETAMINE (MDMA) (MASTER_2020).pdf |
| person | Nor Suliana Mustafa |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15408 |
| sourcemedia | Server storage Scanned document |
| spelling | 15408 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15408 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 Nor Suliana Mustafa Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) Thymoquinone 2020_Effects of Thymoquinone on Neurotoxic Rats Induced By 3, 4 Methylenedioxymethamphetamine (MDMA) 155 EFFECTS OF THYMOQUINONE ON NEUROTOXIC RATS INDUCED BY 3, 4 METHYLENEDIOXYMETHAMPHETAMINE (MDMA) (MASTER_2020).pdf 2020-02-25 Copyright©PWB2025 Ecstasy (3, 4-methylenedioxymethamphetamine; MDMA) is a psychoactive substance that is associated with neurotoxicity. The MDMA exposure on human results in alteration of serotonin (5-HT) released by brain and leads to degeneration of neuronal cells in hippocampus. Most of the treatments available to treat the effects of MDMA are synthetic drugs that can cause severe side effects. An alternative medicine that has less side effects is suggested to treat neurotoxicity caused by MDMA. Thymoquinone (TQ), which is an active compound from Nigella sativa was studied for its protective effect towards neurotoxicity in various models. In spite of that, there is lack of scientific evidences on the effects of TQ in the model of MDMA-induced neurotoxicity. Hence, this study aimed to investigate the effects of TQ on MDMA-induced neurotoxicity based on 5-HT level, sign of anxiety, recognition memory and neuronal damage in rats. The induction of MDMA neurotoxicity was done with two different dosages to stimulate 5-HT depletion and neuronal damage on rats. The administration of TQ into MDMA-induced 5-HT depletion rats was carried out in male Sprague Dawley via 1 week treatment dividing into four groups (n=32, 6-8 per group). The studied groups involved with the treatments comprise i) Control (1 mL/kg saline), ii) MDMA (single administration of 20 mg/kg MDMA), iii) MDMA-TQ (single administration of 20 mg/kg MDMA + 40 mg/kg TQ) and iv) TQ (40 mg/kg TQ). Anxiety test was subjected to a light-dark test and social interaction test. Cerebrospinal fluid was collected from the cisterna magna to determine the level of 5-HT by means of Enzyme-Link Immunosorbent Assay (ELISA). Meanwhile, the administration of TQ into MDMA induced neuronal damage rats was carried out in male Sprague Dawley via 1-week treatment dividing into four groups (n=36, 7-9 per group). The studied groups involved with the treatments comprise i) Control (1 mL/kg saline), ii) MDMA (10 mg/kg MDMA), iii) MDMA-TQ (10 mg/kg MDMA + 40 mg/kg TQ) and iv) TQ (40 mg/kg TQ). A novel object recognition test (NORT) was carried out to measure the memory performance of the rats followed by a histopathological assessment carried out in the hippocampal dentate gyrus by using two staining methods of haematoxylin & eosin (H&E) and Fluoro-Jade C fluorescein. It showed that MDMA caused a significant depletion of 5-HT and neuronal damage when compared to the control (P≤0.05). On the other hand, TQ prevented depletion of 5-HT and reduced anxiety-like behaviours after undergoing a 1-week treatment in MDMA+TQ group as compared to the untreated MDMA group (P≤0.05). The histopathology analysis revealed a statistically significant increase in numbers of positive cells by Fluoro-Jade C following the effect of MDMA on neuronal damage (MDMA induced group) compared to control. Next, the TQ treatments observed in MDMA+TQ exhibited a decline in positive cells from Fluoro Jade C. The index of recognition memory was found to be increased in MDMA+TQ compared to the MDMA alone. This study suggests that the neuronal damage inflicted by MDMA in a rat model has the potential to be treated by TQ. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Thymoquinone — Therapeutic use Neurodegeneration Neuroprotective Effects Experimental Rats Thesis |
| spellingShingle | 2020_Effects of Thymoquinone on Neurotoxic Rats Induced By 3, 4 Methylenedioxymethamphetamine (MDMA) |
| state | Terengganu |
| subject | Dissertations, Academic Thymoquinone — Therapeutic use |
| summary | Ecstasy (3, 4-methylenedioxymethamphetamine; MDMA) is a psychoactive substance that is associated with neurotoxicity. The MDMA exposure on human results in alteration of serotonin (5-HT) released by brain and leads to degeneration of neuronal cells in hippocampus. Most of the treatments available to treat the effects of MDMA are synthetic drugs that can cause severe side effects. An alternative medicine that has less side effects is suggested to treat neurotoxicity caused by MDMA. Thymoquinone (TQ), which is an active compound from Nigella sativa was studied for its protective effect towards neurotoxicity in various models. In spite of that, there is lack of scientific evidences on the effects of TQ in the model of MDMA-induced neurotoxicity. Hence, this study aimed to investigate the effects of TQ on MDMA-induced neurotoxicity based on 5-HT level, sign of anxiety, recognition memory and neuronal damage in rats. The induction of MDMA neurotoxicity was done with two different dosages to stimulate 5-HT depletion and neuronal damage on rats. The administration of TQ into MDMA-induced 5-HT depletion rats was carried out in male Sprague Dawley via 1 week treatment dividing into four groups (n=32, 6-8 per group). The studied groups involved with the treatments comprise i) Control (1 mL/kg saline), ii) MDMA (single administration of 20 mg/kg MDMA), iii) MDMA-TQ (single administration of 20 mg/kg MDMA + 40 mg/kg TQ) and iv) TQ (40 mg/kg TQ). Anxiety test was subjected to a light-dark test and social interaction test. Cerebrospinal fluid was collected from the cisterna magna to determine the level of 5-HT by means of Enzyme-Link Immunosorbent Assay (ELISA). Meanwhile, the administration of TQ into MDMA induced neuronal damage rats was carried out in male Sprague Dawley via 1-week treatment dividing into four groups (n=36, 7-9 per group). The studied groups involved with the treatments comprise i) Control (1 mL/kg saline), ii) MDMA (10 mg/kg MDMA), iii) MDMA-TQ (10 mg/kg MDMA + 40 mg/kg TQ) and iv) TQ (40 mg/kg TQ). A novel object recognition test (NORT) was carried out to measure the memory performance of the rats followed by a histopathological assessment carried out in the hippocampal dentate gyrus by using two staining methods of haematoxylin & eosin (H&E) and Fluoro-Jade C fluorescein. It showed that MDMA caused a significant depletion of 5-HT and neuronal damage when compared to the control (P≤0.05). On the other hand, TQ prevented depletion of 5-HT and reduced anxiety-like behaviours after undergoing a 1-week treatment in MDMA+TQ group as compared to the untreated MDMA group (P≤0.05). The histopathology analysis revealed a statistically significant increase in numbers of positive cells by Fluoro-Jade C following the effect of MDMA on neuronal damage (MDMA induced group) compared to control. Next, the TQ treatments observed in MDMA+TQ exhibited a decline in positive cells from Fluoro Jade C. The index of recognition memory was found to be increased in MDMA+TQ compared to the MDMA alone. This study suggests that the neuronal damage inflicted by MDMA in a rat model has the potential to be treated by TQ. |
| title | 2020_Effects of Thymoquinone on Neurotoxic Rats Induced By 3, 4 Methylenedioxymethamphetamine (MDMA) |
| title_full | 2020_Effects of Thymoquinone on Neurotoxic Rats Induced By 3, 4 Methylenedioxymethamphetamine (MDMA) |
| title_fullStr | 2020_Effects of Thymoquinone on Neurotoxic Rats Induced By 3, 4 Methylenedioxymethamphetamine (MDMA) |
| title_full_unstemmed | 2020_Effects of Thymoquinone on Neurotoxic Rats Induced By 3, 4 Methylenedioxymethamphetamine (MDMA) |
| title_short | 2020_Effects of Thymoquinone on Neurotoxic Rats Induced By 3, 4 Methylenedioxymethamphetamine (MDMA) |
| title_sort | 2020_effects of thymoquinone on neurotoxic rats induced by 3, 4 methylenedioxymethamphetamine (mdma) |