2018_Effect of Apis Dorsata Honey on Development of Morphine Tolerance in Rats: Behavioral and Oxidative Stress Study
| Format: | General Document |
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| _version_ | 1860798008111661056 |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2018-08-19 |
| format | General Document |
| id | 15406 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | EFFECT OF APIS DORSATA HONEY ON DEVELOPMENT OF MORPHINE TOLERANCE IN RATS_ BEHAVIORAL AND OXIDATIVE STRESS STUDY (MASTER_2018).pdf |
| person | Nur Husna Binti Zakaria |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15406 |
| sourcemedia | Server storage Scanned document |
| spelling | 15406 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15406 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 174 2018_Effect of Apis Dorsata Honey on Development of Morphine Tolerance in Rats: Behavioral and Oxidative Stress Study EFFECT OF APIS DORSATA HONEY ON DEVELOPMENT OF MORPHINE TOLERANCE IN RATS_ BEHAVIORAL AND OXIDATIVE STRESS STUDY (MASTER_2018).pdf 2018-08-19 Nur Husna Binti Zakaria Apis Dorsata Honey—Therapeutic use Copyright©PWB2025 Repeated morphine administration can cause tolerance, in which the effect of the drug reduces and causes the need to increase the dose. Previous studies demonstrated the involvement of morphine tolerance with oxidative stress. Production of nitric oxide synthase (NOS) was proven to cause morphine tolerance. The increase of free radicals due to tolerance led to decrease in glutathione (GSH) and antioxidant enzymes, indicating elevated oxidative stress. Honey, a supersaturated solution produced by honeybees possesses rich bioactive compounds, antioxidant, and analgesic effect. Therefore, this study was carried out to determine the effect of A. dorsata honey on the development of morphine tolerance, total glutathione (GSH), malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS) level in the brain of morphine-tolerant rats. Male Sprague Dawley rats were treated into five different groups (n=8) namely saline, morphine, and morphine with honey (0.5, 1.5 & 2.5 g/kg) for 14 days. Assessment of morphine tolerance was carried out using hotplate test. On day 15, the animals were euthanised and the thalamus, spinal cord, and hippocampus were homogenized for the assessment of total GSH, iNOS, and MDA (oxidative stress biomarkers) using ELISA kits. A. dorsata honey significantly prevented morphine tolerance to analgesic in hotplate test on Day 14 (p<0.05). The brain regions biochemical assessment showed that A. dorsata honey prevented GSH depletion compared to morphine control group. A. dorsata honey co-treatment also produced lower MDA compared to morphine control group, with 2.5g/kg served as the significant dose. iNOS increased caused by morphine were lowered in A. dorsata honey co-treatment. A. dorsata honey prevented the development of morphine tolerance with attenuation of oxidative stress biomarkers. This study suggests the therapeutic role of A. dorsata honey in preventing morphine tolerance via inhibition of oxidative stress Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Apis Dorsata Honey And Morphine Tolerance Behavioral Effects Of Morphine Tolerance Pharmacological Effects Of Honey Thesis |
| spellingShingle | 2018_Effect of Apis Dorsata Honey on Development of Morphine Tolerance in Rats: Behavioral and Oxidative Stress Study |
| state | Terengganu |
| subject | Apis Dorsata Honey—Therapeutic use Dissertations, Academic |
| summary | Repeated morphine administration can cause tolerance, in which the effect of the drug reduces and causes the need to increase the dose. Previous studies demonstrated the involvement of morphine tolerance with oxidative stress. Production of nitric oxide synthase (NOS) was proven to cause morphine tolerance. The increase of free radicals due to tolerance led to decrease in glutathione (GSH) and antioxidant enzymes, indicating elevated oxidative stress. Honey, a supersaturated solution produced by honeybees possesses rich bioactive compounds, antioxidant, and analgesic effect. Therefore, this study was carried out to determine the effect of A. dorsata honey on the development of morphine tolerance, total glutathione (GSH), malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS) level in the brain of morphine-tolerant rats. Male Sprague Dawley rats were treated into five different groups (n=8) namely saline, morphine, and morphine with honey (0.5, 1.5 & 2.5 g/kg) for 14 days. Assessment of morphine tolerance was carried out using hotplate test. On day 15, the animals were euthanised and the thalamus, spinal cord, and hippocampus were homogenized for the assessment of total GSH, iNOS, and MDA (oxidative stress biomarkers) using ELISA kits. A. dorsata honey significantly prevented morphine tolerance to analgesic in hotplate test on Day 14 (p<0.05). The brain regions biochemical assessment showed that A. dorsata honey prevented GSH depletion compared to morphine control group. A. dorsata honey co-treatment also produced lower MDA compared to morphine control group, with 2.5g/kg served as the significant dose. iNOS increased caused by morphine were lowered in A. dorsata honey co-treatment. A. dorsata honey prevented the development of morphine tolerance with attenuation of oxidative stress biomarkers. This study suggests the therapeutic role of A. dorsata honey in preventing morphine tolerance via inhibition of oxidative stress |
| title | 2018_Effect of Apis Dorsata Honey on Development of Morphine Tolerance in Rats: Behavioral and Oxidative Stress Study |
| title_full | 2018_Effect of Apis Dorsata Honey on Development of Morphine Tolerance in Rats: Behavioral and Oxidative Stress Study |
| title_fullStr | 2018_Effect of Apis Dorsata Honey on Development of Morphine Tolerance in Rats: Behavioral and Oxidative Stress Study |
| title_full_unstemmed | 2018_Effect of Apis Dorsata Honey on Development of Morphine Tolerance in Rats: Behavioral and Oxidative Stress Study |
| title_short | 2018_Effect of Apis Dorsata Honey on Development of Morphine Tolerance in Rats: Behavioral and Oxidative Stress Study |
| title_sort | 2018_effect of apis dorsata honey on development of morphine tolerance in rats: behavioral and oxidative stress study |