2018_Hydroxychavicol as an Antifungal Agent: Effects on Trichophyton Rubrum and Cytotoxicity
| Format: | General Document |
|---|
| _version_ | 1860798001943937024 |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2018-05-20 |
| format | General Document |
| id | 15375 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | HYDROXYCHAVICOL AS AN ANTIFUNGAL AGENT_ EFFECTS ON TRICHOPHYTON RUBRUM AND CYTOTOXICITY (PHD_2018).pdf |
| person | Muhd Ridzuan Bin Pauzi |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15375 |
| sourcemedia | Server storage Scanned document |
| spelling | 15375 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15375 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Medicine English application/pdf 1.5 223 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) HYDROXYCHAVICOL AS AN ANTIFUNGAL AGENT_ EFFECTS ON TRICHOPHYTON RUBRUM AND CYTOTOXICITY (PHD_2018).pdf 2018_Hydroxychavicol as an Antifungal Agent: Effects on Trichophyton Rubrum and Cytotoxicity 2018-05-20 Antifungal agents Hydroxychavicol Cytotoxicity Copyright©PWB2025 Muhd Ridzuan Bin Pauzi Trichophyton rubrum is one of the most prevalent dermatophytes causing both local and systemic infection. Current available antifungal agents may cause severe side effects and emergence of resistant strains. Hydroxychavicol (HC) is an active compound of Piper leaf species. It has been reported to have antifungal properties against dermatophytes including T. rubrum. The properties of HC and its mechanisms of action as an antifungal agent against T. rubrum has not been comprehensively explained. Hence this study is aimed to identify the effects of HC as an antifungal agent against T. rubrum. Broth dilution method was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of HC against T. rubrum (ATCC 28188). The morphology of T. rubrum was then studied using transmission electron microscope (TEM). On the other hand, fungal cytoskeletal enzymes (chitin synthase) gene expressions were evaluated using real-time PCR. Subsequently, cytotoxicity studies using human dermal fibroblast (HDF) and pre adipocyte (3T3-L1) cell lines were performed by means of MTT cell proliferation assay. The level of MIC and MFC of the HC against T. rubrum was 0.49µg/mL. Subsequently, microscopic study revealed significant destruction of the fungal organelles and cell wall. The fungal cytoskeletal enzyme (chitin synthase) gene expressions showed upregulated at Day-1 and down regulated from Day-5 until Day-7 of treatment. On the other hand, HC was less toxic when compared to miconazole as the reference antifungal agent to the tested cell lines (HDF and 3T3-L1). Administration of HC led to T. rubrum cell wall distruption and organelles destruction as well as changes in the expression of the chitin synthase gene. Hence, HC could potentially be used as an alternative therapeutic agent against T. rubrum infections. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Trichophyton Rubrum Thesis |
| spellingShingle | 2018_Hydroxychavicol as an Antifungal Agent: Effects on Trichophyton Rubrum and Cytotoxicity |
| state | Terengganu |
| subject | Antifungal agents Dissertations, Academic |
| summary | Trichophyton rubrum is one of the most prevalent dermatophytes causing both local and systemic infection. Current available antifungal agents may cause severe side effects and emergence of resistant strains. Hydroxychavicol (HC) is an active compound of Piper leaf species. It has been reported to have antifungal properties against dermatophytes including T. rubrum. The properties of HC and its mechanisms of action as an antifungal agent against T. rubrum has not been comprehensively explained. Hence this study is aimed to identify the effects of HC as an antifungal agent against T. rubrum. Broth dilution method was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of HC against T. rubrum (ATCC 28188). The morphology of T. rubrum was then studied using transmission electron microscope (TEM). On the other hand, fungal cytoskeletal enzymes (chitin synthase) gene expressions were evaluated using real-time PCR. Subsequently, cytotoxicity studies using human dermal fibroblast (HDF) and pre adipocyte (3T3-L1) cell lines were performed by means of MTT cell proliferation assay. The level of MIC and MFC of the HC against T. rubrum was 0.49µg/mL. Subsequently, microscopic study revealed significant destruction of the fungal organelles and cell wall. The fungal cytoskeletal enzyme (chitin synthase) gene expressions showed upregulated at Day-1 and down regulated from Day-5 until Day-7 of treatment. On the other hand, HC was less toxic when compared to miconazole as the reference antifungal agent to the tested cell lines (HDF and 3T3-L1). Administration of HC led to T. rubrum cell wall distruption and organelles destruction as well as changes in the expression of the chitin synthase gene. Hence, HC could potentially be used as an alternative therapeutic agent against T. rubrum infections. |
| title | 2018_Hydroxychavicol as an Antifungal Agent: Effects on Trichophyton Rubrum and Cytotoxicity |
| title_full | 2018_Hydroxychavicol as an Antifungal Agent: Effects on Trichophyton Rubrum and Cytotoxicity |
| title_fullStr | 2018_Hydroxychavicol as an Antifungal Agent: Effects on Trichophyton Rubrum and Cytotoxicity |
| title_full_unstemmed | 2018_Hydroxychavicol as an Antifungal Agent: Effects on Trichophyton Rubrum and Cytotoxicity |
| title_short | 2018_Hydroxychavicol as an Antifungal Agent: Effects on Trichophyton Rubrum and Cytotoxicity |
| title_sort | 2018_hydroxychavicol as an antifungal agent: effects on trichophyton rubrum and cytotoxicity |