2024_Association of BDNF(RS6265) with Obesity in Adult Pakistani Population and Its Molecular Docking with Tropomyocin Receptor Kinase B (TrkB)
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
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| country | Malaysia |
| date | 2024-07-30 |
| format | General Document |
| id | 15368 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | ASSOCIATION OF BDNF(rs6265) WITH OBESITY IN ALDULT PAKISTANI POPULATION AND ITS MOLECULAR DOCKING WITH TROPOMYOCIN RECEPTOR KINASE B (TrkB) (MASTER_ 2024).pdf |
| person | Faheem Mustapha |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15368 |
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| spelling | 15368 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15368 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Pharmacy English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 3.0.10; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 127 ASSOCIATION OF BDNF(rs6265) WITH OBESITY IN ALDULT PAKISTANI POPULATION AND ITS MOLECULAR DOCKING WITH TROPOMYOCIN RECEPTOR KINASE B (TrkB) (MASTER_ 2024).pdf 2024_Association of BDNF(RS6265) with Obesity in Adult Pakistani Population and Its Molecular Docking with Tropomyocin Receptor Kinase B (TrkB) Faheem Mustapha BDNF (RS6265) Obesity—Genetic aspects—Pakistan Copyright©PWB2025 2024-07-30 Problem statement: A rapid increase in obesity prevalence has been observed in Pakistan. According to a WHO report, in 2016, 20.8% population was overweight (BMI 25-29.9), and 4.8% were obese (BMI >30) in Pakistan. Multiple genetic and environmental factors disrupt energy homeostasis and increase body weight. BDNF regulates leptinproopiomelanocortin which then regulating energy homeostasis, BMI, and lipid parameters. Polymorphism of BDNF Val66Met rs6265 can disrupt energy homeostasis and alter BMI and lipid parameters. Hyperphagia and obesity were observed in BDNF heterozygous mice. The primary goal of this study was to investigate the association of BDNF rs6265 with anthropometric and lipid profiles in obese Pakistani subjects. Methodology: This case-control study was conducted in the Pakistani population aged 20-59 years. Random samples were taken from volunteers. A total of 66 obese and 66 control subjects having a BMI ≥25 kg/m² (for obese) and 18.5-22.9 kg/m² (for normal healthy individuals) were included. The study was approved by UniSZA Human Research Ethics Committee UniSZA (UHREC/2022/388), followed by the approval from Office of Research Innovation and Commercialization (ORIC) at the UMT, Lahore (RE-009-2021). In-silico analysis of wild and mutant BDNF protein and its receptor TrkB was blind-docked with ClusPro 2.0. Anthropometric data, including weight, height, waist circumference, hip circumference, and neck circumference were measured. A total of 4 ml blood samples were collected from median cubital vein. Genotyping analysis of variation in BDNF (rs6265) was determined by using PCR-RFLP. Clinical characteristics of the sample population were expressed as mean ±SD. Statistical analysis was performed by using SPSS 21.0. Results: Docking results showed that the rs6265 polymorphism in BDNF reduced the binding affinity between the BDNF and TrkB. Obese attributes had significantly greater neck circumference (P<0.05), body fat percentage (P<0.05), and lower high-density lipoprotein (P<0.05), as compared to healthy individuals. Significant association was observed between genotypic variation rs6265 and hip circumference, waist circumference, BMI, body fat percentage, and cholesterol (P<0.05). The AA genotype in obese subjects had the highest count contributing 56% of the obese samples, followed by GG, having a frequency of 44%. Non-obese subjects revealed the highest frequency (70%) of the GG genotype and 30% of the AA genotype. There was also an apparent significant difference in both allelic (P<0.005) and genotypic (P<0.05) frequencies between the obese and normal subjects. Conclusion: The docking result showed that the rs6265 polymorphism in BDNF reduced the binding affinity between BDNF and TrkB. BDNF rs6265 genotypes showed a significant association between obesity and its attributes. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Tropomyosin Receptor Kinase B (TrkB) Brain-Derived Neurotrophic Factor Thesis |
| spellingShingle | 2024_Association of BDNF(RS6265) with Obesity in Adult Pakistani Population and Its Molecular Docking with Tropomyocin Receptor Kinase B (TrkB) |
| state | Terengganu |
| subject | Obesity—Genetic aspects—Pakistan Dissertations, Academic |
| summary | Problem statement: A rapid increase in obesity prevalence has been observed in Pakistan. According to a WHO report, in 2016, 20.8% population was overweight (BMI 25-29.9), and 4.8% were obese (BMI >30) in Pakistan. Multiple genetic and environmental factors disrupt energy homeostasis and increase body weight. BDNF regulates leptinproopiomelanocortin which then regulating energy homeostasis, BMI, and lipid parameters. Polymorphism of BDNF Val66Met rs6265 can disrupt energy homeostasis and alter BMI and lipid parameters. Hyperphagia and obesity were observed in BDNF heterozygous mice. The primary goal of this study was to investigate the association of BDNF rs6265 with anthropometric and lipid profiles in obese Pakistani subjects. Methodology: This case-control study was conducted in the Pakistani population aged 20-59 years. Random samples were taken from volunteers. A total of 66 obese and 66 control subjects having a BMI ≥25 kg/m² (for obese) and 18.5-22.9 kg/m² (for normal healthy individuals) were included. The study was approved by UniSZA Human Research Ethics Committee UniSZA (UHREC/2022/388), followed by the approval from Office of Research Innovation and Commercialization (ORIC) at the UMT, Lahore (RE-009-2021). In-silico analysis of wild and mutant BDNF protein and its receptor TrkB was blind-docked with ClusPro 2.0. Anthropometric data, including weight, height, waist circumference, hip circumference, and neck circumference were measured. A total of 4 ml blood samples were collected from median cubital vein. Genotyping analysis of variation in BDNF (rs6265) was determined by using PCR-RFLP. Clinical characteristics of the sample population were expressed as mean ±SD. Statistical analysis was performed by using SPSS 21.0. Results: Docking results showed that the rs6265 polymorphism in BDNF reduced the binding affinity between the BDNF and TrkB. Obese attributes had significantly greater neck circumference (P<0.05), body fat percentage (P<0.05), and lower high-density lipoprotein (P<0.05), as compared to healthy individuals. Significant association was observed between genotypic variation rs6265 and hip circumference, waist circumference, BMI, body fat percentage, and cholesterol (P<0.05). The AA genotype in obese subjects had the highest count contributing 56% of the obese samples, followed by GG, having a frequency of 44%. Non-obese subjects revealed the highest frequency (70%) of the GG genotype and 30% of the AA genotype. There was also an apparent significant difference in both allelic (P<0.005) and genotypic (P<0.05) frequencies between the obese and normal subjects. Conclusion: The docking result showed that the rs6265 polymorphism in BDNF reduced the binding affinity between BDNF and TrkB. BDNF rs6265 genotypes showed a significant association between obesity and its attributes. |
| title | 2024_Association of BDNF(RS6265) with Obesity in Adult Pakistani Population and Its Molecular Docking with Tropomyocin Receptor Kinase B (TrkB) |
| title_full | 2024_Association of BDNF(RS6265) with Obesity in Adult Pakistani Population and Its Molecular Docking with Tropomyocin Receptor Kinase B (TrkB) |
| title_fullStr | 2024_Association of BDNF(RS6265) with Obesity in Adult Pakistani Population and Its Molecular Docking with Tropomyocin Receptor Kinase B (TrkB) |
| title_full_unstemmed | 2024_Association of BDNF(RS6265) with Obesity in Adult Pakistani Population and Its Molecular Docking with Tropomyocin Receptor Kinase B (TrkB) |
| title_short | 2024_Association of BDNF(RS6265) with Obesity in Adult Pakistani Population and Its Molecular Docking with Tropomyocin Receptor Kinase B (TrkB) |
| title_sort | 2024_association of bdnf(rs6265) with obesity in adult pakistani population and its molecular docking with tropomyocin receptor kinase b (trkb) |