2022_Monitoring Invasive Peacock Bass (Cichla spp.) In Malaysia Through Molecular DNA Methods
| Format: | General Document |
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| _version_ | 1860797996093931520 |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2022-10-02 |
| format | General Document |
| id | 15347 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | 15347_33a6e7cf651ffed.pdf |
| person | Aliyu Garba Khaleel |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15347 |
| sourcemedia | Server storage Scanned document |
| spelling | 15347 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15347 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 242 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 15347_33a6e7cf651ffed.pdf 2022_Monitoring Invasive Peacock Bass (Cichla spp.) In Malaysia Through Molecular DNA Methods Aliyu Garba Khaleel Copyright©PWB2025 2022-10-02 The establishment of feral population of invasive Cichla spp. is associated with the declining of local aquatic species in Malaysia. Monitoring the Cichla spp. is critical to minimize the effect it may cause to the local biodiversity. Conventional monitoring, morphology-based identification and impact evaluation are time-consuming, invasive and financially expensive. Introduction of molecular approach such as environmental DNA (eDNA) method offers more rapid monitoring approach within a shorter time. DNA barcoding and genetic diversity studies are necessary for the developing a reliable genus-specific primer set for detecting Cichla spp. Objectives of this study were; i) to determine the origin, genetic diversity, propagule pressure and population structure of invasive Cichla spp. by utilising molecular approach, ii) to evaluate the diet content and impact of Cichla spp. in Lake Telabak biodiversity, and iii) to develop a genus-specific primer for Cichla spp. from mitochondrial DNA region for eDNA application. A total of 186 Cichla spp. were captured from different areas of Peninsular Malaysia for this study. Mitochondrial DNA analyses was used to determine the origin, genetic diversity, propagule pressure and population structure of Cichla spp. DNA barcoding technique was used for the stomach content analysis. A genus-specific primer was designed using a Primer3 Pro software and validated by amplifying targeted Cichla spp. sequence and six non-targeted fish species to ensure only Cichla spp. sample was amplified. Aquarium and field water samples were used for in vitro and in situ workability tests for designed primer, respectively. The findings revealed the origin of Cichla spp. in Lake Telabak as Cichla ocellaris (Bloch and Schneider, 1801) with a sequence similarity of 99.72%. High genetic diversity (Hd, 0.6700 and π, 0.0254), multiple propagule pressure and two population structures were observed among Cichla spp. in Malaysia. Seven prey species (six fishes 43.0% and one shrimp 5.1%) belongs to five families were identified in fishes’ stomach. A genus-specific primer designed was successfully yielded positive results by detecting only Cichla spp. for water samples collected from both aquarium and field. The molecular approaches were successfully utilised to determine origin, genetic diversity, propagule pressure and population structure of Cichla spp. Besides, several prey items consist of fishes and shrimp were detected in the Cichla spp. stomach. Finally, the genus specific primer set was successfully designed and can be used to detect Cichla spp. both in aquarium as well as in the field. Peacock bass Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Invasive Species Monitoring Peacock Bass (Cichla Spp.) Molecular DNA Methods Thesis |
| spellingShingle | 2022_Monitoring Invasive Peacock Bass (Cichla spp.) In Malaysia Through Molecular DNA Methods |
| state | Terengganu |
| subject | Peacock bass Dissertations, Academic |
| summary | The establishment of feral population of invasive Cichla spp. is associated with the declining of local aquatic species in Malaysia. Monitoring the Cichla spp. is critical to minimize the effect it may cause to the local biodiversity. Conventional monitoring, morphology-based identification and impact evaluation are time-consuming, invasive and financially expensive. Introduction of molecular approach such as environmental DNA (eDNA) method offers more rapid monitoring approach within a shorter time. DNA barcoding and genetic diversity studies are necessary for the developing a reliable genus-specific primer set for detecting Cichla spp. Objectives of this study were; i) to determine the origin, genetic diversity, propagule pressure and population structure of invasive Cichla spp. by utilising molecular approach, ii) to evaluate the diet content and impact of Cichla spp. in Lake Telabak biodiversity, and iii) to develop a genus-specific primer for Cichla spp. from mitochondrial DNA region for eDNA application. A total of 186 Cichla spp. were captured from different areas of Peninsular Malaysia for this study. Mitochondrial DNA analyses was used to determine the origin, genetic diversity, propagule pressure and population structure of Cichla spp. DNA barcoding technique was used for the stomach content analysis. A genus-specific primer was designed using a Primer3 Pro software and validated by amplifying targeted Cichla spp. sequence and six non-targeted fish species to ensure only Cichla spp. sample was amplified. Aquarium and field water samples were used for in vitro and in situ workability tests for designed primer, respectively. The findings revealed the origin of Cichla spp. in Lake Telabak as Cichla ocellaris (Bloch and Schneider, 1801) with a sequence similarity of 99.72%. High genetic diversity (Hd, 0.6700 and π, 0.0254), multiple propagule pressure and two population structures were observed among Cichla spp. in Malaysia. Seven prey species (six fishes 43.0% and one shrimp 5.1%) belongs to five families were identified in fishes’ stomach. A genus-specific primer designed was successfully yielded positive results by detecting only Cichla spp. for water samples collected from both aquarium and field. The molecular approaches were successfully utilised to determine origin, genetic diversity, propagule pressure and population structure of Cichla spp. Besides, several prey items consist of fishes and shrimp were detected in the Cichla spp. stomach. Finally, the genus specific primer set was successfully designed and can be used to detect Cichla spp. both in aquarium as well as in the field. |
| title | 2022_Monitoring Invasive Peacock Bass (Cichla spp.) In Malaysia Through Molecular DNA Methods |
| title_full | 2022_Monitoring Invasive Peacock Bass (Cichla spp.) In Malaysia Through Molecular DNA Methods |
| title_fullStr | 2022_Monitoring Invasive Peacock Bass (Cichla spp.) In Malaysia Through Molecular DNA Methods |
| title_full_unstemmed | 2022_Monitoring Invasive Peacock Bass (Cichla spp.) In Malaysia Through Molecular DNA Methods |
| title_short | 2022_Monitoring Invasive Peacock Bass (Cichla spp.) In Malaysia Through Molecular DNA Methods |
| title_sort | 2022_monitoring invasive peacock bass (cichla spp.) in malaysia through molecular dna methods |