2022_Characterization and Risk Assessment of Listeria Monocytogenes in Chicken Offal from Wet Markets and Supermarkets Around Klang Valley, Malaysia
| Format: | General Document |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2022-10-07 |
| format | General Document |
| id | 15338 |
| institution | UniSZA |
| internalnotes | Sila masukkan subject wajib Dissertations, Academic. Terima kasih... |
| originalfilename | 15338_270bc463d51114b.pdf |
| person | Wai Ga Yarn |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15338 |
| sourcemedia | Server storage Scanned document |
| spelling | 15338 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15338 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.5 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin 209 SAMBox 2.4.24; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 2022-10-07 15338_270bc463d51114b.pdf 2022_Characterization and Risk Assessment of Listeria Monocytogenes in Chicken Offal from Wet Markets and Supermarkets Around Klang Valley, Malaysia Listeria monocytogenes Risk Assessment Food Safety Wai Ga Yarn Copyright©PWB2025 Listeria monocytogenes is an important emerging foodborne pathogen with infections causing high mortality rates (20-30%). It can lead to serious cases of listeriosis especially among pregnant women and the elderly. The emergence of multiple antibiotic resistant strains further complicate treatment of listeriosis cases. The goals of this present study include: a) To detect and characterize Listeria spp. and Listeria monocytogenes as hazard in chicken offal, b) To characterize the antibiotic resistance profiles of L. monocytogenes isolates, c) To assess the risk of listeriosis among general population, pregnant women, elderly and immunocompromised from chicken offal consumption and, d) To evaluate the food safety knowledge, attitude and practices (KAP) among consumers. Multiplex Polymerase Chain Reaction (PCR) assay and plating method were used to detect and isolate Listeria spp. and L. monocytogenes in chicken offal from several wet markets and supermarkets in the Klang Valley. Prevalence data was used to determine the probability of listeriosis from chicken offal consumption using beta-Poisson model. Antibiotic resistance profile of L. monocytogenes isolates was characterized based on 13 types of antibiotics. The cluster analysis was analysed using Pyelph 1.4 software based on similarity index. Survivability of L. monocytogenes ATCC7644 and L. monocytogenes AMPG1 were determined for refrigeration (4oC) storage and temperature abuse (28oC). The data obtained was used to calculate the probability of infection among vulnerable groups using exponential model. Monte Carlo simulation with 10,000 iteration was used to determine the distribution of expected cases and rate of infection. An online survey using Google form was conducted to determine the level of knowledge, attitudes and practices among 101 consumers in Malaysia. Prevalence of L. monocytogenes at wet markets and supermarkets was 0.5% and 11.3%, respectively. The level of contamination in chicken gizzard, liver and heart was 13.6%, 10.5% and 1.5%, respectively. Beta-Poisson analysis showed possible sporadic cases of listeriosis from chicken offal consumption. The rate of illness per 100,000 population caused by chicken offal consumption from wet markets and supermarkets were 0.15 and 3.36, respectively. The estimated rate of illness per 100,000 population for gizzard, liver and heart was 3.09, 2.08 and 0.45 respectively. L. monocytogenes isolates showed multiple antibiotic resistance patterns with 71.43% of the isolates having a MAR index above 0.20. Cluster analysis showed the isolates exhibited similar resistance patterns which can be differentiated into three clusters at a 70% cut-off similarity. L. monocytogenes AMPG1 showed similar growth pattern with L. monocytogenes ATCC 7644 inoculated on chicken liver stored at 4oC and 28oC. Mean growth of L. monocytogenes at 4oC and 28oC were 0.40±0.11 and 0.18±0.04, respectively. Monte Carlo simulation showed L. monocytogenes in chicken liver at refrigerated and room temperature increased by 20.50-fold and 243.06-fold, respectively. Immunocompromised patients were estimated to show the highest risk of listeriosis followed by pregnant women, elderly and the general population. The results of KAP survey showed that there was significantly positive correlation among the three levels and consumers possess good knowledge (71.29% ± 24.2) and attitudes (67.14% ± 18.8) but average on practices (54.0% ± 18.9). This study concluded that the presence of L. monocytogenes in chicken offal along with inadequate safe food practices among consumers pose a significant risk of listeriosis. Dissertations, Academic Sila masukkan subject wajib Dissertations, Academic. Terima kasih... Listeria Monocytogenes Thesis |
| spellingShingle | 2022_Characterization and Risk Assessment of Listeria Monocytogenes in Chicken Offal from Wet Markets and Supermarkets Around Klang Valley, Malaysia |
| state | Terengganu |
| subject | Listeria monocytogenes Dissertations, Academic |
| summary | Listeria monocytogenes is an important emerging foodborne pathogen with infections causing high mortality rates (20-30%). It can lead to serious cases of listeriosis especially among pregnant women and the elderly. The emergence of multiple antibiotic resistant strains further complicate treatment of listeriosis cases. The goals of this present study include: a) To detect and characterize Listeria spp. and Listeria monocytogenes as hazard in chicken offal, b) To characterize the antibiotic resistance profiles of L. monocytogenes isolates, c) To assess the risk of listeriosis among general population, pregnant women, elderly and immunocompromised from chicken offal consumption and, d) To evaluate the food safety knowledge, attitude and practices (KAP) among consumers. Multiplex Polymerase Chain Reaction (PCR) assay and plating method were used to detect and isolate Listeria spp. and L. monocytogenes in chicken offal from several wet markets and supermarkets in the Klang Valley. Prevalence data was used to determine the probability of listeriosis from chicken offal consumption using beta-Poisson model. Antibiotic resistance profile of L. monocytogenes isolates was characterized based on 13 types of antibiotics. The cluster analysis was analysed using Pyelph 1.4 software based on similarity index. Survivability of L. monocytogenes ATCC7644 and L. monocytogenes AMPG1 were determined for refrigeration (4oC) storage and temperature abuse (28oC). The data obtained was used to calculate the probability of infection among vulnerable groups using exponential model. Monte Carlo simulation with 10,000 iteration was used to determine the distribution of expected cases and rate of infection. An online survey using Google form was conducted to determine the level of knowledge, attitudes and practices among 101 consumers in Malaysia. Prevalence of L. monocytogenes at wet markets and supermarkets was 0.5% and 11.3%, respectively. The level of contamination in chicken gizzard, liver and heart was 13.6%, 10.5% and 1.5%, respectively. Beta-Poisson analysis showed possible sporadic cases of listeriosis from chicken offal consumption. The rate of illness per 100,000 population caused by chicken offal consumption from wet markets and supermarkets were 0.15 and 3.36, respectively. The estimated rate of illness per 100,000 population for gizzard, liver and heart was 3.09, 2.08 and 0.45 respectively. L. monocytogenes isolates showed multiple antibiotic resistance patterns with 71.43% of the isolates having a MAR index above 0.20. Cluster analysis showed the isolates exhibited similar resistance patterns which can be differentiated into three clusters at a 70% cut-off similarity. L. monocytogenes AMPG1 showed similar growth pattern with L. monocytogenes ATCC 7644 inoculated on chicken liver stored at 4oC and 28oC. Mean growth of L. monocytogenes at 4oC and 28oC were 0.40±0.11 and 0.18±0.04, respectively. Monte Carlo simulation showed L. monocytogenes in chicken liver at refrigerated and room temperature increased by 20.50-fold and 243.06-fold, respectively. Immunocompromised patients were estimated to show the highest risk of listeriosis followed by pregnant women, elderly and the general population. The results of KAP survey showed that there was significantly positive correlation among the three levels and consumers possess good knowledge (71.29% ± 24.2) and attitudes (67.14% ± 18.8) but average on practices (54.0% ± 18.9). This study concluded that the presence of L. monocytogenes in chicken offal along with inadequate safe food practices among consumers pose a significant risk of listeriosis. |
| title | 2022_Characterization and Risk Assessment of Listeria Monocytogenes in Chicken Offal from Wet Markets and Supermarkets Around Klang Valley, Malaysia |
| title_full | 2022_Characterization and Risk Assessment of Listeria Monocytogenes in Chicken Offal from Wet Markets and Supermarkets Around Klang Valley, Malaysia |
| title_fullStr | 2022_Characterization and Risk Assessment of Listeria Monocytogenes in Chicken Offal from Wet Markets and Supermarkets Around Klang Valley, Malaysia |
| title_full_unstemmed | 2022_Characterization and Risk Assessment of Listeria Monocytogenes in Chicken Offal from Wet Markets and Supermarkets Around Klang Valley, Malaysia |
| title_short | 2022_Characterization and Risk Assessment of Listeria Monocytogenes in Chicken Offal from Wet Markets and Supermarkets Around Klang Valley, Malaysia |
| title_sort | 2022_characterization and risk assessment of listeria monocytogenes in chicken offal from wet markets and supermarkets around klang valley, malaysia |