2019_Bioremediatio of Industrial Textile Dyes Effluent by Bacillus Species With Process Optimization
| Format: | General Document |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2019-10-15 16:15 |
| format | General Document |
| id | 15292 |
| institution | UniSZA |
| originalfilename | BIOREMEDIATION OF INDUSTRIAL TEXTILE DYES EFFLUENT BY BACILLUS SPECIES WITH PROCESS OPTIMIZATION |
| person | PDFsam Basic v4.2.10 Nur Hanis Mohamad Hanapi |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15292 |
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| spelling | 15292 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15292 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu English UniSZA East Coast Environmental Research Institute application/pdf 1.5 PDFsam Basic v4.2.10 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin SAMBox 2.3.4; modified using iTextSharp™ 5.5.10 ©2000-2016 iText Group NV (AGPL-version) 2019-10-15 16:15 262 BIOREMEDIATION OF INDUSTRIAL TEXTILE DYES EFFLUENT BY BACILLUS SPECIES WITH PROCESS OPTIMIZATION 2019_Bioremediatio of Industrial Textile Dyes Effluent by Bacillus Species With Process Optimization Copyright©PWB2025 Textile industry—Waste disposal Bioremediation Industrial wastewater—Purification Nur Hanis Mohamad Hanapi Bioremediation Industrial Textile Dyes Dyes Effluent Bacillus Species Process Optimization Dyes and dyeing—Environmental aspects Bacillus (Bacteria)—Biotechnology Microbial biotechnology Water—Pollution—Biodegradation Wastewater treatment—Biological treatment Environmental microbiology Process optimization—Biotechnology Textile industries is one of the contributing factors in water pollution. Production of textiles which dated centuries ago, witnessed the application of colours to the textiles industries. Textile dyes can be grouped into natural and synthetic dyes. The natural type of dyes is usually hard to be acquired since it were derived from plants, low in production and time consuming. Due to the textiles production beginning to bloom and expands throughout the world, the invention of synthetic dyes has started to evolve and conquered the textile industries. It is cheap, robust and easy to be processed as compared to the natural dyes which acquired from plant sources such as roots, berries, barks, leaves, woo, fungi and lichens. The increasing demands in textile industries created huge demands of textile coloured products. However, large production of untreated textile effluent caused the water bodies to be affected with the colouring agents thus creating danger to the environment since the synthetic dyes used are usually hard to be broken down into lesser dangerous components. This will cause harm to the aquatic life especially, since they are most to be affected with this water pollution. In this study, biological treatment of textile wastewater using microorganisms was applied. Three potential local bacterial isolates were isolated from the textile wastewater (raw and activated sludge tank), which acquired from local textile wastewater treatment plant. Biochemical tests, antibiotic resistance, polymerase chain reaction (PCR) and electrophoresis gel were performed for the bacterial identification process. Two statistical analyses namely two-level full factorial and central composite designs (CCD) from response surface methodology (RSM) were applied in order to find the optimized treatment process condition for the textile dyes wastewater degradation. For two levels factorial design, a total of thirty-two runs of experiments with five factors were performed. Bacterial inoculum (%, v/v), temperature (℃), agitation (rpm), pH and aeration (aerobic/anaerobic) were tested. It was observed that during the ten days incubation of preliminary screening, 90-95% of textile dyes and COD were able to be removed in the textile wastewater. Three bacterial isolates were successfully identified through DNA sequencing procedures and were identified as Bacillus species according to their band length (~1500kbp). It was observed that during preliminary screening, degradation of textile dye and COD removal were achieved with 52.74% and 86% respectively with different process conditions. Highest dye degradation was achieved with pH 10, 15% (v/v) of initial bacterial inoculum, 30 ℃ and 200 rpm while COD removal required initial pH solution at pH 4, with 5% (v/v) of initial bacterial inoculum with same temperature and agitation speed. Anaerobic condition was preferred in both conditions. The process optimization with CCD gave 99.2% of COD removal with pH 10, 10% of bacterial inoculum and 100 rpm agitation speed while colour degradation was achieved with 88.67% with pH 4, 10% (v/v) of bacterial inoculum and 100 rpm of agitation speed. The optimized process condition in colour and chemical oxygen demand (COD) degradation with biological treatment by Bacillus sp. confirmed that almost complete degradation of textile dyes and COD can be achieved and completed. Dissertations, Academic Thesis |
| spellingShingle | 2019_Bioremediatio of Industrial Textile Dyes Effluent by Bacillus Species With Process Optimization |
| state | Terengganu |
| subject | Textile industry—Waste disposal Bioremediation Industrial wastewater—Purification Dyes and dyeing—Environmental aspects Bacillus (Bacteria)—Biotechnology Microbial biotechnology Water—Pollution—Biodegradation Wastewater treatment—Biological treatment Environmental microbiology Process optimization—Biotechnology Dissertations, Academic |
| summary | Textile industries is one of the contributing factors in water pollution. Production of textiles which dated centuries ago, witnessed the application of colours to the textiles industries. Textile dyes can be grouped into natural and synthetic dyes. The natural type of dyes is usually hard to be acquired since it were derived from plants, low in production and time consuming. Due to the textiles production beginning to bloom and expands throughout the world, the invention of synthetic dyes has started to evolve and conquered the textile industries. It is cheap, robust and easy to be processed as compared to the natural dyes which acquired from plant sources such as roots, berries, barks, leaves, woo, fungi and lichens. The increasing demands in textile industries created huge demands of textile coloured products. However, large production of untreated textile effluent caused the water bodies to be affected with the colouring agents thus creating danger to the environment since the synthetic dyes used are usually hard to be broken down into lesser dangerous components. This will cause harm to the aquatic life especially, since they are most to be affected with this water pollution. In this study, biological treatment of textile wastewater using microorganisms was applied. Three potential local bacterial isolates were isolated from the textile wastewater (raw and activated sludge tank), which acquired from local textile wastewater treatment plant. Biochemical tests, antibiotic resistance, polymerase chain reaction (PCR) and electrophoresis gel were performed for the bacterial identification process. Two statistical analyses namely two-level full factorial and central composite designs (CCD) from response surface methodology (RSM) were applied in order to find the optimized treatment process condition for the textile dyes wastewater degradation. For two levels factorial design, a total of thirty-two runs of experiments with five factors were performed. Bacterial inoculum (%, v/v), temperature (℃), agitation (rpm), pH and aeration (aerobic/anaerobic) were tested. It was observed that during the ten days incubation of preliminary screening, 90-95% of textile dyes and COD were able to be removed in the textile wastewater. Three bacterial isolates were successfully identified through DNA sequencing procedures and were identified as Bacillus species according to their band length (~1500kbp). It was observed that during preliminary screening, degradation of textile dye and COD removal were achieved with 52.74% and 86% respectively with different process conditions. Highest dye degradation was achieved with pH 10, 15% (v/v) of initial bacterial inoculum, 30 ℃ and 200 rpm while COD removal required initial pH solution at pH 4, with 5% (v/v) of initial bacterial inoculum with same temperature and agitation speed. Anaerobic condition was preferred in both conditions. The process optimization with CCD gave 99.2% of COD removal with pH 10, 10% of bacterial inoculum and 100 rpm agitation speed while colour degradation was achieved with 88.67% with pH 4, 10% (v/v) of bacterial inoculum and 100 rpm of agitation speed. The optimized process condition in colour and chemical oxygen demand (COD) degradation with biological treatment by Bacillus sp. confirmed that almost complete degradation of textile dyes and COD can be achieved and completed. |
| title | 2019_Bioremediatio of Industrial Textile Dyes Effluent by Bacillus Species With Process Optimization |
| title_full | 2019_Bioremediatio of Industrial Textile Dyes Effluent by Bacillus Species With Process Optimization |
| title_fullStr | 2019_Bioremediatio of Industrial Textile Dyes Effluent by Bacillus Species With Process Optimization |
| title_full_unstemmed | 2019_Bioremediatio of Industrial Textile Dyes Effluent by Bacillus Species With Process Optimization |
| title_short | 2019_Bioremediatio of Industrial Textile Dyes Effluent by Bacillus Species With Process Optimization |
| title_sort | 2019_bioremediatio of industrial textile dyes effluent by bacillus species with process optimization |