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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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2024-08-30 11:25:22
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15238
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UniSZA
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1. Hussain MS, Fareed S, Ansari S, Rahman MA, Ahmad IZ, Saeed M, Current Approaches toward Production of Secondary Plant Metabolites, Journal of Pharmacy and Bioallied Sciences, 4, 2012, 10-20. 2. 2. Schmidt TJ, Khalid SA, Romanha AJ, Alves TMA, Biavatti MW, Brun R, Da Costa FB, De Castro SL, Ferreira VF, De Lacerda MVG, Lago JHG, Leon LL, Lopes NP, Das Neves Amorim RC, Niehues M, Ogungbe IV, Pohlit AM, Scotti MT, Setzer WN, De NC, Soeiro M, Steindel M, Tempone AG, The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases - Part II, Current Medicinal Chemistry, 19, 2012, 2176-2228. 3. Rasool Hassan BA, Medicinal Plants (Importance and Uses), Pharmaceutica Analytica Acta, 3, 2012, e139. doi:10.4172/2153-2435.1000e139. 4. Phillipson JD, Phytochemistry and Medicinal Plants, Phytochemistry, 56, 2001, 237−243. 5. Middleton DJ, An update on the Apocynaceae in Thailand, Thai Forest Bulletin (Botany), Special Issue, 2009, 143-155. 6. Tao LP, Leeuwenberg AJM, Middleton DJ, Apocynaceae, Flora of China, 16, 1995, 143-188. 7. Tan MJ, Yin C, Tang CP, Ke CQ, Lin G, Ye Y, Antitussive Indole Alkaloids from Kopsia hainanensis, Planta Medica, 77, 2011, 939-944. 8. Lim KH, Hiraku O, Komiyama K, Koyano T, Hayashi M, Kam TS, Biologically Active Indole Alkaloids from Kopsia arborea, Journal of Natural Products, 70, 2007, 1302-1307. 9. Wu Y, Kitajima M, Kogure N, Wang Y, Zhang R, Takayama H, Two New Aspidosperma Indole Alkaloids from Yunnan Kopsia arborea, Chemical and Pharmaceutical Bulletin, 58, 2010, 961-963. 10. Subramaniam G, Kam TS, New Indole Alkaloids from Kopsia. Alkaloids Variation in Kopsia singapurensis, Helvetica Chimica Acta, 91, 2008, 930-937. 11. Awang K, Ahmad K, Thomas NF, Hirasawa Y, Takeya K, Mukhtar MR, Mohamad K, Morita H, Singaporentine A: A New Indole Alkaloid from Kopsia singapurensis Ridl, Heterocycles, 75, 2008, 3051-3056. 12. Kam TS, Subramaniam G, Chen W, Alkaloids from Kopsia dasyrachis, Phytochemistry, 51, 1999, 159-169. 13. Yap WS, Gan CY, Low YY, Choo YM, Etoh T, Hayashi M, Komiyama K, Kam TS, Grandilodines A−C, Biologically Active Indole Alkaloids from Kopsia grandifolia, Journal of Natural Products, 74, 2011, 1309-1312. 14. Kaushik NK, Kaushik N, Attri P, Kumar N, Kim CH, Verma AK, Choi EH, Biomedical Importance of Indoles, Molecules, 18, 2013, 6620-6662. 15. Kitajima M, Murakami Y, Takahashi N, Wu Y, Kogure N, Zhang RP, Takayama H, Asymmetric Total Synthesis of Novel Pentacyclic Indole Alkaloid, Kopsiyunnanine E, Isolated from Kopsia arborea, Organic Letters, 16, 2014, 5000-5003. 16. Schultz EE, Pujanauski BG, Sarpong R, Synthetic Studies toward Lapidilectine-type Kopsia Alkaloids, Organic Letters, 14, 2012, 648-651. 17. Leal RA, Beaudry DR, Alzghari SK, Sarpong R, Synthesis of the Pentacyclic Skeleton of the Indole Alkaloid Arboflorine, Organic Letters, 14, 2012, 5350-5353. 18. Arai S, Nakajima M, Nishida A, A Concise and Versatile Synthesis of Alkaloids from Kopsia tenuis: Total Synthesis of (±)-Lundurine A and B, Angewandte Chemies, 126, 2014, 5675-5678. 19. Ahmad K, Hirasawa Y, Nugroho AE, Hadi AHA, Morita H, New Aspidofractinine, Aspidospermatan and Akuamiline Indole Alkaloids from the Roots of Kopsia singapurensis Ridl., Heterocycles, 86, 2012, 1611-1619. 20. Lim KH, Komiyama K, Kam TS, Arboricine and Arboricinine, Unusual Tetracyclic Indole Regioisomers from Kopsia, Tetrahedron Letters, 48, 2007, 1143-1145. 21. Subramaniam G, Kam TS, Mersinaline and Mersirachine, Novel Quinolinic Alkaloids of the Mersinine Group from Kopsia, Tetrahedron Letters, 48, 2007, 6677-6680. 22. Low YY, Subramaniam G, Lim KH, Wong RCS, Robinson WT, Kam TS, Mersiphyllines A and B from Kopsia. Determination of Relative Configuration at A Quaternary Center via Formation of an Alkaloid-borane Complex, Tetrahedron, 65, 2009, 6873-6876. 23. Ahmad K, Hirasawa Y, Nugroho AE, Hadi AHA, Takeya K, Thomas NF, Awang K, Morita H, Tan SP, Nafiah MA, New Indole Alkaloids from Kopsia Singapurensis (Ridl.), The Open Conference Proceedings Journal, 4 (Suppl-2, M18), 2013, 75-82. 24. Lee YS, Tee CT, Tan SP, Awang K, Hashim NM, Nafiah MA, Ahmad K, Cytotoxic, Antibacterial and Antioxidant Activity of Triterpenoids from Kopsia singapurensis Ridl, Journal of Chemical and Pharmaceutical Research, 6, 2014, 815-822. 25. Lamchouri F, Zemzami M, Jossang A, Settaf A, Israili ZH, Lyoussi B, Cytotoxicity of alkaloids isolated from Peganum harmala seeds, Pakistan Journal of Pharmaceutical Sciences, 26, 2013, 699-706. 26. Tajudin, TJSA, Mat N, Aishah ABS, Yusran AZM, Alwi A, Ali AM, Cytotoxicity, Antiproliferative Effects, and Apoptosis Induction of Methanolic Extract of Cynometra cauliflora Linn. Whole Fruit on Human Promyelocytic Leukemia HL-60 Cells, Evidence-Based Complementary and Alternative Medicine, 2012, 2012, 1-6. 27. Kam TS, Choo YM, Chen W, Yao JX, Indole and Monoterpene Alkaloids from the Leaves of Kopsia dasyrachis, Phytochemistry, 52, 1999, 959-963. 28. Lim KH, Hiraku O, Komiyama K, Koyano T, Hayashi M, Kam TS, Biologically Active Indole Alkaloids from Kopsia arborea, Journal of Natural Products, 70, 2007, 1302-1307. 29. Kam TS, Subramaniam G, Sire KM, Yoganathan K, Koyano T, Toyoshima M, Rho MC, Hayashi M, Komiyama K, Reversal of Multidrug Resistance (MDR) by Aspidofractinine-Type Indole Alkaloids, Bioorganic & Medicinal Chemistry Letters, 8, 1998, 2769-2772. 30. Subramaniam G, Hiraku O, Hayashi M, Koyano T, Komiyama K, Kam TS, Biologically Active Aspidofractinine, Rhazinilam, Akuammiline, and Vincorine Alkaloids from Kopsia, Journal of Natural Products, 70, 2007, 1783-1789.
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6031-01-FH02-FBIM-15-03316.pdf
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Adobe Acrobat 20.6
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15238
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15238 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=15238 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf Adobe Acrobat Pro DC 20 Paper Capture Plug-in with ClearScan 8 1.6 Adobe Acrobat 20.6 2024-08-30 11:25:22 6031-01-FH02-FBIM-15-03316.pdf UniSZA Private Access In vitro Cytotoxic Effect of Indole Alkaloids from The Roots of Kopsia singapurensis ridl. Against The Human Promyelocytic leukemia (HL-60) and The Human Cervical Cancer (HeLa) Cells International Journal of Pharmaceutical Sciences Review and Research Six indole alkaloids; kopsamine N(4)-oxide 1, kopsifine 2, rhazinicine 3, aspidodasycarpine 4, kopsamine 5 and akuammidine 6, were isolated from the roots of Kopsia singapurensis Ridl. (Apocynaceae). Compound 2 is first time isolated from the roots of K. singapurensis Ridl. Those compounds were screened for in vitro cytotoxic activity against human cervical cancer (HeLa), human promyelocytic leukemia (HL-60), and the normal mouse fibroblast (NIH/3T3) cell lines by using the MTT assay. Based on comparison with vincristine (control), kopsifine 2 showed very strong cytotoxic effects against HL-60 cells, while, kopsamine 5 exhibited a moderate cytotoxic activity effects against HL-60 cells with CD50 values of 0.9 μg/mL and 6.9 μg/mL, respectively. Akuammidine 6 and rhazinicine 3 exhibited significant cytotoxicity effects against HeLa cells with the CD50 values 2.8 μg/mL and 2.9 μg/mL, respectively. However, aspidodasycarpine 4 showed a moderate cytotoxicity effects against HeLa cells with the CD50 value of 7.5 μg/mL, and kopsamine N(4)-oxide 1 was inactive against all these cancer cells. Although kopsifine 2, rhazinicine 3, and aspidodasycarpine 4 showed high cytotoxic effects against HL-60 and HeLa cell lines, but they also showed cytotoxic on normal cell (NIH/3T3) with CD50 values of 20.7, 20.8, and 6.4 μg/mL, respectively. Therefore, these three alkaloids are not suggested as cytotoxic agents for these cancer cells. This is the first cytotoxicity report on HL-60, HeLa and NIH/3T3 cell lines for these compounds. 31 2 Global Research Online Global Research Online 89-95 1. Hussain MS, Fareed S, Ansari S, Rahman MA, Ahmad IZ, Saeed M, Current Approaches toward Production of Secondary Plant Metabolites, Journal of Pharmacy and Bioallied Sciences, 4, 2012, 10-20. 2. 2. Schmidt TJ, Khalid SA, Romanha AJ, Alves TMA, Biavatti MW, Brun R, Da Costa FB, De Castro SL, Ferreira VF, De Lacerda MVG, Lago JHG, Leon LL, Lopes NP, Das Neves Amorim RC, Niehues M, Ogungbe IV, Pohlit AM, Scotti MT, Setzer WN, De NC, Soeiro M, Steindel M, Tempone AG, The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases - Part II, Current Medicinal Chemistry, 19, 2012, 2176-2228. 3. Rasool Hassan BA, Medicinal Plants (Importance and Uses), Pharmaceutica Analytica Acta, 3, 2012, e139. doi:10.4172/2153-2435.1000e139. 4. Phillipson JD, Phytochemistry and Medicinal Plants, Phytochemistry, 56, 2001, 237−243. 5. Middleton DJ, An update on the Apocynaceae in Thailand, Thai Forest Bulletin (Botany), Special Issue, 2009, 143-155. 6. Tao LP, Leeuwenberg AJM, Middleton DJ, Apocynaceae, Flora of China, 16, 1995, 143-188. 7. Tan MJ, Yin C, Tang CP, Ke CQ, Lin G, Ye Y, Antitussive Indole Alkaloids from Kopsia hainanensis, Planta Medica, 77, 2011, 939-944. 8. Lim KH, Hiraku O, Komiyama K, Koyano T, Hayashi M, Kam TS, Biologically Active Indole Alkaloids from Kopsia arborea, Journal of Natural Products, 70, 2007, 1302-1307. 9. Wu Y, Kitajima M, Kogure N, Wang Y, Zhang R, Takayama H, Two New Aspidosperma Indole Alkaloids from Yunnan Kopsia arborea, Chemical and Pharmaceutical Bulletin, 58, 2010, 961-963. 10. Subramaniam G, Kam TS, New Indole Alkaloids from Kopsia. Alkaloids Variation in Kopsia singapurensis, Helvetica Chimica Acta, 91, 2008, 930-937. 11. Awang K, Ahmad K, Thomas NF, Hirasawa Y, Takeya K, Mukhtar MR, Mohamad K, Morita H, Singaporentine A: A New Indole Alkaloid from Kopsia singapurensis Ridl, Heterocycles, 75, 2008, 3051-3056. 12. Kam TS, Subramaniam G, Chen W, Alkaloids from Kopsia dasyrachis, Phytochemistry, 51, 1999, 159-169. 13. Yap WS, Gan CY, Low YY, Choo YM, Etoh T, Hayashi M, Komiyama K, Kam TS, Grandilodines A−C, Biologically Active Indole Alkaloids from Kopsia grandifolia, Journal of Natural Products, 74, 2011, 1309-1312. 14. Kaushik NK, Kaushik N, Attri P, Kumar N, Kim CH, Verma AK, Choi EH, Biomedical Importance of Indoles, Molecules, 18, 2013, 6620-6662. 15. Kitajima M, Murakami Y, Takahashi N, Wu Y, Kogure N, Zhang RP, Takayama H, Asymmetric Total Synthesis of Novel Pentacyclic Indole Alkaloid, Kopsiyunnanine E, Isolated from Kopsia arborea, Organic Letters, 16, 2014, 5000-5003. 16. Schultz EE, Pujanauski BG, Sarpong R, Synthetic Studies toward Lapidilectine-type Kopsia Alkaloids, Organic Letters, 14, 2012, 648-651. 17. Leal RA, Beaudry DR, Alzghari SK, Sarpong R, Synthesis of the Pentacyclic Skeleton of the Indole Alkaloid Arboflorine, Organic Letters, 14, 2012, 5350-5353. 18. Arai S, Nakajima M, Nishida A, A Concise and Versatile Synthesis of Alkaloids from Kopsia tenuis: Total Synthesis of (±)-Lundurine A and B, Angewandte Chemies, 126, 2014, 5675-5678. 19. Ahmad K, Hirasawa Y, Nugroho AE, Hadi AHA, Morita H, New Aspidofractinine, Aspidospermatan and Akuamiline Indole Alkaloids from the Roots of Kopsia singapurensis Ridl., Heterocycles, 86, 2012, 1611-1619. 20. Lim KH, Komiyama K, Kam TS, Arboricine and Arboricinine, Unusual Tetracyclic Indole Regioisomers from Kopsia, Tetrahedron Letters, 48, 2007, 1143-1145. 21. Subramaniam G, Kam TS, Mersinaline and Mersirachine, Novel Quinolinic Alkaloids of the Mersinine Group from Kopsia, Tetrahedron Letters, 48, 2007, 6677-6680. 22. Low YY, Subramaniam G, Lim KH, Wong RCS, Robinson WT, Kam TS, Mersiphyllines A and B from Kopsia. Determination of Relative Configuration at A Quaternary Center via Formation of an Alkaloid-borane Complex, Tetrahedron, 65, 2009, 6873-6876. 23. Ahmad K, Hirasawa Y, Nugroho AE, Hadi AHA, Takeya K, Thomas NF, Awang K, Morita H, Tan SP, Nafiah MA, New Indole Alkaloids from Kopsia Singapurensis (Ridl.), The Open Conference Proceedings Journal, 4 (Suppl-2, M18), 2013, 75-82. 24. Lee YS, Tee CT, Tan SP, Awang K, Hashim NM, Nafiah MA, Ahmad K, Cytotoxic, Antibacterial and Antioxidant Activity of Triterpenoids from Kopsia singapurensis Ridl, Journal of Chemical and Pharmaceutical Research, 6, 2014, 815-822. 25. Lamchouri F, Zemzami M, Jossang A, Settaf A, Israili ZH, Lyoussi B, Cytotoxicity of alkaloids isolated from Peganum harmala seeds, Pakistan Journal of Pharmaceutical Sciences, 26, 2013, 699-706. 26. Tajudin, TJSA, Mat N, Aishah ABS, Yusran AZM, Alwi A, Ali AM, Cytotoxicity, Antiproliferative Effects, and Apoptosis Induction of Methanolic Extract of Cynometra cauliflora Linn. Whole Fruit on Human Promyelocytic Leukemia HL-60 Cells, Evidence-Based Complementary and Alternative Medicine, 2012, 2012, 1-6. 27. Kam TS, Choo YM, Chen W, Yao JX, Indole and Monoterpene Alkaloids from the Leaves of Kopsia dasyrachis, Phytochemistry, 52, 1999, 959-963. 28. Lim KH, Hiraku O, Komiyama K, Koyano T, Hayashi M, Kam TS, Biologically Active Indole Alkaloids from Kopsia arborea, Journal of Natural Products, 70, 2007, 1302-1307. 29. Kam TS, Subramaniam G, Sire KM, Yoganathan K, Koyano T, Toyoshima M, Rho MC, Hayashi M, Komiyama K, Reversal of Multidrug Resistance (MDR) by Aspidofractinine-Type Indole Alkaloids, Bioorganic & Medicinal Chemistry Letters, 8, 1998, 2769-2772. 30. Subramaniam G, Hiraku O, Hayashi M, Koyano T, Komiyama K, Kam TS, Biologically Active Aspidofractinine, Rhazinilam, Akuammiline, and Vincorine Alkaloids from Kopsia, Journal of Natural Products, 70, 2007, 1783-1789.
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In vitro Cytotoxic Effect of Indole Alkaloids from The Roots of Kopsia singapurensis ridl. Against The Human Promyelocytic leukemia (HL-60) and The Human Cervical Cancer (HeLa) Cells
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| summary |
Six indole alkaloids; kopsamine N(4)-oxide 1, kopsifine 2, rhazinicine 3, aspidodasycarpine 4, kopsamine 5 and akuammidine 6, were isolated from the roots of Kopsia singapurensis Ridl. (Apocynaceae). Compound 2 is first time isolated from the roots of K. singapurensis Ridl. Those compounds were screened for in vitro cytotoxic activity against human cervical cancer (HeLa), human promyelocytic leukemia (HL-60), and the normal mouse fibroblast (NIH/3T3) cell lines by using the MTT assay. Based on comparison with vincristine (control), kopsifine 2 showed very strong cytotoxic effects against HL-60 cells, while, kopsamine 5 exhibited a moderate cytotoxic activity effects against HL-60 cells with CD50 values of 0.9 μg/mL and 6.9 μg/mL, respectively. Akuammidine 6 and rhazinicine 3 exhibited significant cytotoxicity effects against HeLa cells with the CD50 values 2.8 μg/mL and 2.9 μg/mL, respectively. However, aspidodasycarpine 4 showed a moderate cytotoxicity effects against HeLa cells with the CD50 value of 7.5 μg/mL, and kopsamine N(4)-oxide 1 was inactive against all these cancer cells. Although kopsifine 2, rhazinicine 3, and aspidodasycarpine 4 showed high cytotoxic effects against HL-60 and HeLa cell lines, but they also showed cytotoxic on normal cell (NIH/3T3) with CD50 values of 20.7, 20.8, and 6.4 μg/mL, respectively. Therefore, these three alkaloids are not suggested as cytotoxic agents for these cancer cells. This is the first cytotoxicity report on HL-60, HeLa and NIH/3T3 cell lines for these compounds.
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| title |
In vitro Cytotoxic Effect of Indole Alkaloids from The Roots of Kopsia singapurensis ridl. Against The Human Promyelocytic leukemia (HL-60) and The Human Cervical Cancer (HeLa) Cells
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| title_full |
In vitro Cytotoxic Effect of Indole Alkaloids from The Roots of Kopsia singapurensis ridl. Against The Human Promyelocytic leukemia (HL-60) and The Human Cervical Cancer (HeLa) Cells
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| title_fullStr |
In vitro Cytotoxic Effect of Indole Alkaloids from The Roots of Kopsia singapurensis ridl. Against The Human Promyelocytic leukemia (HL-60) and The Human Cervical Cancer (HeLa) Cells
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| title_full_unstemmed |
In vitro Cytotoxic Effect of Indole Alkaloids from The Roots of Kopsia singapurensis ridl. Against The Human Promyelocytic leukemia (HL-60) and The Human Cervical Cancer (HeLa) Cells
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| title_short |
In vitro Cytotoxic Effect of Indole Alkaloids from The Roots of Kopsia singapurensis ridl. Against The Human Promyelocytic leukemia (HL-60) and The Human Cervical Cancer (HeLa) Cells
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| title_sort |
in vitro cytotoxic effect of indole alkaloids from the roots of kopsia singapurensis ridl. against the human promyelocytic leukemia (hl-60) and the human cervical cancer (hela) cells
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