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1860797880625790976
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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2024-08-26 14:39:46
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Restricted Document
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14501
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UniSZA
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2141-01-FH02-FPBSM-14-00664.pdf
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Adobe Acrobat 20.6
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=14501
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14501 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=14501 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 3 Adobe Acrobat Pro DC 20 Paper Capture Plug-in with ClearScan 1.6 Adobe Acrobat 20.6 2024-08-26 14:39:46 2141-01-FH02-FPBSM-14-00664.pdf UniSZA Private Access Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis Journal of plant physiology We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1- chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABAinduced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca 2+ -permeable channel currents by ABA or oscillation of the cytosolic free Ca 2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca 2+ oscillation in ABA signal pathway of Arabidopsis guard cells.
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Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis
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| summary |
We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1- chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABAinduced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca 2+ -permeable channel currents by ABA or oscillation of the cytosolic free Ca 2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca 2+ oscillation in ABA signal pathway of Arabidopsis guard cells.
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| title |
Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis
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| title_full |
Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis
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| title_fullStr |
Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis
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| title_full_unstemmed |
Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis
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| title_short |
Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis
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| title_sort |
negative regulation of abscisic acid-induced stomatal closure by glutathione in arabidopsis
|