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1860797513756311552
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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2024-08-27 16:05:09
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Restricted Document
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13043
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UniSZA
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7355-01-FH02-FBIM-20-45671.pdf
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uniszai7-user
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=13043
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13043 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=13043 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 11 1.6 uniszai7-user Adobe Acrobat Pro DC 20.6.20042 2024-08-27 16:05:09 7355-01-FH02-FBIM-20-45671.pdf UniSZA Private Access Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media Journal Of Agrobiotechnology Dioscorea hispida Dennst produces tuber which possess valuable medicinal properties but unsustainable harvesting has led to its reduction. The plant propagates slowly because of its low tuber sprouting rate. In average, Dioscorea hispida Dennst tubers took approximately 60 d to break dormancy and sprout. Hence, callus culture is proposed as a possible efficient type of culture for manipulation of this species. In the present study, calli were induced from stem segments to evaluate callus culture potential of Dioscorea hispida Dennst. Results indicate that the combination of 1 mgL-1 naphthaleneacetic acid (NAA), 1 mgL-1 6- benzylaminopurine (BAP) and 0.5 mgL-1 2,4-dichlorophenoxyacetic acid (2, 4-D) in Gamborg (B5) medium improved callus multiplication and differentiation in the stem culture as opposed to those in Murashige and Skoog (MS) medium. The findings from the present study provide the basis of callus culture protocol for stem explant of Dioscorea hispida Dennst with B5 being the more effective basal medium. 11 2 Penerbit Universiti Sultan Zainal Abidin Penerbit Universiti Sultan Zainal Abidin 30-38
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Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media
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| summary |
Dioscorea hispida Dennst produces tuber which possess valuable medicinal properties but unsustainable harvesting has led to its reduction. The plant propagates slowly because of its low tuber sprouting rate. In average, Dioscorea hispida Dennst tubers took approximately 60 d to break dormancy and sprout. Hence, callus culture is proposed as a possible efficient type of culture for manipulation of this species. In the present study, calli were induced from stem segments to evaluate callus culture potential of Dioscorea hispida Dennst. Results indicate that the combination of 1 mgL-1 naphthaleneacetic acid (NAA), 1 mgL-1 6- benzylaminopurine (BAP) and 0.5 mgL-1 2,4-dichlorophenoxyacetic acid (2, 4-D) in Gamborg (B5) medium improved callus multiplication and differentiation in the stem culture as opposed to those in Murashige and Skoog (MS) medium. The findings from the present study provide the basis of callus culture protocol for stem explant of Dioscorea hispida Dennst with B5 being the more effective basal medium.
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| title |
Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media
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| title_full |
Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media
|
| title_fullStr |
Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media
|
| title_full_unstemmed |
Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media
|
| title_short |
Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media
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| title_sort |
shoot generation and callus induction of dioscorea hispida dennst by different plant growth hormones and basal media
|