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1860797494453075968
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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| date |
2020-10-30 17:36:16
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| format |
Restricted Document
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12964
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UniSZA
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7272-01-FH02-FBIM-20-43240.pdf
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uniszai7-user
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12964
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12964 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12964 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 15 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 1.7 uniszai7-user 2020-10-30 17:36:16 7272-01-FH02-FBIM-20-43240.pdf UniSZA Private Access Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology Malaysian Journal of Applied Sciences Many researchers have focused chitosan as a source of potential bioactive material during the past few decades. However, chitosan has several drawbacks to be utilised in biological applications, including poor solubility under physiological conditions. Therefore, a new interest has recently emerged on partially hydrolysed chitosan, chitosan oligosaccharides (COS). In this study, degradation of chitosan was performed by Cellulase from Trichoderma reesei® 1.5L and Response Surface Methodology (RSM) were employed to optimize the hydrolysis temperature, pH, enzyme concentration and substrate concentration. Optimization of cellulase T. reesei® using central composite design (CCD) was to obtain optimum parameters and all the factors showed significant effects (p˂0.05). The maximum response, Celluclast® activity (1.268 U) was obtained by assaying the process at 49.79oC, pH 4.5, 3% (v/w) of enzyme concentration and 25% (w/v) concentration of chitosan for 24 hours. 5 2 Penerbit Universiti Sultan Zainal Abidin Penerbit Universiti Sultan Zainal Abidin 30-44
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| spellingShingle |
Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology
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| summary |
Many researchers have focused chitosan as a source of potential bioactive material during the past few decades. However, chitosan has several drawbacks to be utilised in biological applications, including poor solubility under physiological conditions. Therefore, a new interest has recently emerged on partially hydrolysed chitosan, chitosan oligosaccharides (COS). In this study, degradation of chitosan was performed by Cellulase from Trichoderma reesei® 1.5L and Response Surface Methodology (RSM) were employed to optimize the hydrolysis temperature, pH, enzyme concentration and substrate concentration. Optimization of cellulase T. reesei® using central composite design (CCD) was to obtain optimum parameters and all the factors showed significant effects (p˂0.05). The maximum response, Celluclast® activity (1.268 U) was obtained by assaying the process at 49.79oC, pH 4.5, 3% (v/w) of enzyme concentration and 25% (w/v) concentration of chitosan for 24 hours.
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| title |
Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology
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| title_full |
Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology
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| title_fullStr |
Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology
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| title_full_unstemmed |
Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology
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| title_short |
Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology
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| title_sort |
production of chitosan oligosaccharides using β-glycosidic degrading enzyme: optimization using response surface methodology
|