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1860797492599193600
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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| date |
2020-06-14 21:01:34
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| format |
Restricted Document
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| id |
12957
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UniSZA
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7264-01-FH02-FSK-20-43201.pdf
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osama
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12957
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12957 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12957 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 6 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 6 osama 2020-06-14 21:01:34 7264-01-FH02-FSK-20-43201.pdf UniSZA Private Access Effects of Trigona honey on the Gene Expression Profile of Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615 Jordan Journal of Biological Sciences Honey is a broad-spectrum antimicrobial agent that seems to affect different bacteria in many different ways. The aim of this study was to evaluate the antibacterial activities of Trigona honey against P. aeruginosa and S. pyogenes. The effect of Trigona honey on P. aeruginosa and S. pyogenes was investigated using growth kinetics curve and real-time PCR. The growth kinetics of P. aeruginosa and S. pyogenes with 20% (w/v)(MIC) Trigona honey inhibited the growth cells number of P. aeruginosa and S. pyogenes compared with cells grown without honey. Treatment with 10% (w/v) (half-MIC) showed slightly decreased number of cells of P. aeruginosa and S. pyogenes over a period of 24 hours. Conversely, treatment with 5% (w/v) (quarter-MIC) was observed to have a similar untreated samples of P. aeruginosa and S. pyogenes. The RT-qPCR results showed that the expression of Sof and Sfbl decreased 7.82-fold and 9.23-fold respectively after exposure to 20% concentration of Trigona honey, whereas the expression of algD and oprF decreased 6.28-fold and 11.11-fold respectively after exposure to 20% concentration of Trigona honey. Trigona honey demonstrated the highest antibacterial activity against P. aeruginosa and S. pyogenes in vitro. Our results indicate that Trigona honey has the potential to be an effective inhibitor on virulence genes of P. aeruginosa and S. pyogenes. 13 2 133-138
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| spellingShingle |
Effects of Trigona honey on the Gene Expression Profile of Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615
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| subject |
6
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| summary |
Honey is a broad-spectrum antimicrobial agent that seems to affect different bacteria in many different ways. The aim of this study was to evaluate the antibacterial activities of Trigona honey against P. aeruginosa and S. pyogenes. The effect of Trigona honey on P. aeruginosa and S. pyogenes was investigated using growth kinetics curve and real-time PCR. The growth kinetics of P. aeruginosa and S. pyogenes with 20% (w/v)(MIC) Trigona honey inhibited the growth cells number of P. aeruginosa and S. pyogenes compared with cells grown without honey. Treatment with 10% (w/v) (half-MIC) showed slightly decreased number of cells of P. aeruginosa and S. pyogenes over a period of 24 hours. Conversely, treatment with 5% (w/v) (quarter-MIC) was observed to have a similar untreated samples of P. aeruginosa and S. pyogenes. The RT-qPCR results showed that the expression of Sof and Sfbl decreased 7.82-fold and 9.23-fold respectively after exposure to 20% concentration of Trigona honey, whereas the expression of algD and oprF decreased 6.28-fold and 11.11-fold respectively after exposure to 20% concentration of Trigona honey. Trigona honey demonstrated the highest antibacterial activity against P. aeruginosa and S. pyogenes in vitro. Our results indicate that Trigona honey has the potential to be an effective inhibitor on virulence genes of P. aeruginosa and S. pyogenes.
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| title |
Effects of Trigona honey on the Gene Expression Profile of Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615
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| title_full |
Effects of Trigona honey on the Gene Expression Profile of Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615
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| title_fullStr |
Effects of Trigona honey on the Gene Expression Profile of Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615
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| title_full_unstemmed |
Effects of Trigona honey on the Gene Expression Profile of Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615
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| title_short |
Effects of Trigona honey on the Gene Expression Profile of Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615
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| title_sort |
effects of trigona honey on the gene expression profile of pseudomonas aeruginosa atcc 10145 and streptococcus pyogenes atcc 19615
|