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2016-04-27 10:38:36
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12917
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UniSZA
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[1] Amarowicz, R., Naczk, M. et al. Antioxidant activity of condensed tannins of beach pea, canola hulls, evening primrose and Faba bean. Journal Food Lipids. 2000; 7: 195–205. [2] Arora-Kuruganti, P., Lucchesi, P. A. and Wurster, R. D. Proliferation of Cultured Human Astrocytoma Cells in Response to an Oxidant and Antioxidant. Journal of Neuro-Oncology. 1999;44 (3): 213–221. [3] Atangwho, I. J., Ebong, P. E., Egbung, G. E., Eteng, M. U. and Eyong, M. U. Effects of Vernonia amygdalina Del:leaf on liver function in alloxan induced hyperglycemic rats. J. Pharm. Bioresource. 2007c; 4(1): 1-7. [4] Atangwho, I. J., Ebong, P. E., Eyong, E. U., Eteng, M. U. and Obi, A. U. Effects of Vernonia amygdalina Del:leaf on kidney function of diabetic rats. Int. J. Pharm. 2007b; 3(2):143- 148. [5] Atangwho IJ, Ebong PO, et al. Vernonia amygdalina Del: A potential prophylactic antidiabetic agent in lipids complication. Glob. J Pure Applied Sci. 2007a; 3(1); 103-106. [6] Boeing, H., Bechthold, A., Bub, A. et al. Critical review: vegetables and fruit in the prevention of chronic diseases. European Journal of Nutrition. 2012; 51 (6): 637–663. [7] Buchanan DR, White JD, et al. Research-design issues in cancer-symptom-management trials using complementary and alternative medicine: lessons from the National Cancer Institute Community Clinical Oncology Program experience. Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology. 2005; 23 (27): 6682–9. [8] Chen, D., Pace, P. E., Coombes, R. C. and Ali, S. Phosphorylation of human estrogen receptor alpha by protein kinase A regulates dimerization. Molecular Cell Biology. 1999; 19 (2): 1002-15. [9] Duan, X., Wu, G. and Jiang, Y. Evaluation of the antioxidant properties of litchi fruit phenolics in relation to pericarp browning prevention. Molecules. 2007; 12 (4): 759-771. [10] Eleonora, T., Lorenzo, F. and Carmela, F. Potential effects of pomegranate polyphenols in cancer prevention and therapy. Oxidative medicine and cellular longevity. 2015:1-19. [11] Farombi, E. O. and Owoeye, O. Antioxidative and chemopreventive properties of Vernonia amygdalina and Garcinia biflavonoid. Int J Environ Res Public Health. 2011; 8(6): 2533-55. [12] Gawron, A. and Kruk, G. Cytotoxic effect of xanthotoxol 8-hydroxypsoralen on TCTC cells in vitro. Polish Journal of Pharmacology and Pharmacy. 1992; 44 (1): 51-57. [13] Geran, R. I., Greenberg, H. M., McDonald, M. and Abbott, B. J. Protocols for screening chemical agents and natural products against animal tumours and other biological systems. Cancer Chemoth Rep. 1972; 33: 1-17. [14] Gresham, L. J., Ross, J. and Izevbigie, E. B. Vernonia amygdalina; anticancer activity, authentication and adulteration detection. Int J Environ Res Public Health. 2008; 5(5): 342-8. [15] Grubben, G. J. H. Prota volume 2- vegetables, prota foundation. Wageningen. 2004: 543-544. [16] Harborne, J. B. Arsenal for Survival: Secondary Plant Products. Taxon. 2000; 49 (3): 435. [17] Kanatt, S. R., Chander, R. and Sharma, A. Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products. International Journal of Food Science & Technology. 2010; 45 (2): 216–222. [18] Khalili, M. A., Hadi, N. A., Naim, R., Baig, A. A. and Mahmud, K. Study on antioxidant capacity and anticancer activity of Bismillah leaf (Vernonia amygdalina). World Journal of Pharmaceutical Research. 2014; 3 (6): 14-29. [19] Khoshyomn, S., et al. Synergistic effect of genistein and BCNU on growth inhibition and cytotoxicity of glioblastoma cells. Journal of Neuro-Oncology. 2002; 57 (3): 193–200. [20] Li, H., Wang, X., Li, P., Li, Y. and Wang, H. Polyphenolics compounds and antioxidant properties of selected china wines. Food chemistry. 2009; 112 (2): 454-460. [21] Livingstone, J. Natural compounds in cancer therapy. International Journal Of Pharmaceutical Medicine. 2001; 15. [22] Lu, F. and Foo, L.Y. Toxicological aspects of food antioxidants. In: Madhavi DL, Deshpande SS, Salunkhe DK, editors. Food antioxidants. New York: Marcel Dekker. 1995. [23] Luo, X., Jiang, Y., Fronczek, F. R., Lin, C., Izevbigie, E. B. and Lee, K. S. Isolation and structure determination of a sesquiterpene lactone (vernodalinol) from Vernonia amygdalina extracts. Pharm boil. 2011; 49 (5): 464-70. [24] Luo, X., Oyugi, D. A., Lin, C., Izevbigie, E. B. and Lee, K. S. Isolation and characterization of the antibreast carcinoma cell growth components of Vernonia amygdalina extracts. Exp Boil Med (Maywood). 2010; 235 (12): 1472-8. [25] Mohd, Zin, Z., Abdul Hamid, A. and Osman, A. Antioxidative activity of extracts from mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food. Chem. 2002; 78: 227-231. [26] Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983; 65 (1-2): 55–63. [27] Mohd Adzim Khalili Rohin, Norhayati Abd Hadi, Rochman Naim, Atif Amin Baig, Khairil Mahmud: Study On Antioxidant Capacity And Anticancer Activity Of Bismillah Leaf (Vernonia Amygdalina). World Journal of Pharmaceutical Research 09/2014; 3(6):14-29. [28] Mohd Adzim Khalili R, Che Abdullah A.B, And Abdul Manaf A: Total Antioxidant Activity, Total Phenolic Content And Radical Scavenging Activity Both Flesh And Peel Of Red Pitaya, White Pitaya And Papaya. International Journal of Pharmacy and Pharmaceutical Sciences 04/2012; Vol 4,(Issue 2):113-122. [29] Mohd Adzim Khalili R, Norhayati AH, et al. Determination of radical scavenging activity and Vitamin A, C and E in organically grown Red Pitaya (Hylocereus sp.). International Food Research Journal 06/2010; Volume 17.(Issue 2.-ISSN 22317546):405-409. [30] Nwaoguikpe, R. N. The effect of extract of bitter leaf (Vernonia amygdalina) on blood glucose levels of diabetic rats. Int. Journal Biology Chemical Science. 2010; 4 (3): 721-729. [31] Oyugi, D. A., Luo, X., Lee, K. S., Hill, B. and Izevbigie, E. B. Activity markers of the anti-breast carcinoma cell growth fractions of Vernonia amygdalina extracts. Exp Boil Med (Maywood). 2009; 234 (4): 410-7. [32] Pan, Y., Wang, K., Huang, S., et al. Antioxidant activity of micro-wave assisted extract of Longan (Dimocarpus longan lour.) peel. Food chemistry. 2008;106 (3): 1264-1270. [33] Phyllistin AB, James FB.. Tips for preventing food poisoning “Herbs” American No.1 Guide Natural health, 3rd edn. Publ. Averge, 2000; 9: 383-386. [34] Richardson MA, et al. Complementary/Alternative Medicine Use in a Comprehensive Cancer Center and the Implications for Oncology. J. Clin. Oncol.. 2000; 18 (13): 2505–2514. [35] Scheck, A. C., Perry, K., Hank, N. C. and Clark, W. D. Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells. BMC Complementary and Alternative Medicine. 2006; 6 (27). [36] Scheck, A. C., Shapiro, J. R., Coons, S. W., Norman, S. A. and Johnson, P. C. Biological and molecular analysis of a low-grade recurrence of a glioblastoma multiforme. Clinical Cancer Research : An Official Journal of the American Association for Cancer Research. 1996; 2 (1):187–99. [37] Schiffers, R.R. African indigenous vegetables: an overview of the cultivated species. University Greenwich Press, England. 2000. [38] Schwartsmann, G., Winograd, B. and Pinedo, H. M. The main steps in the development of anticancer agents. Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology. 1988;12 (4): 301–13. [39] Turrini, E., Ferruzzi, L. and Fimognari, C. Potential Effects of Pomegranate Polyphenols in Cancer Prevention and Therapy. 2015. [40] Yedjou, C., Izevbigie, E. and Tchounwou, P. Preclinical assessment of Vernonia amygdalina leaf extracts as DNA damaging anti-cancer agent in the management of breast cancer. Int J Environ Res Public Health. 2008; 5(5): 337-41. [41] Zakaria ZA, et al. In vitro antistaphylococcal activity of the extracts of several neglected plants in Malaysia. International Journal of Pharmacology. 2007; 3 (5): 428-431. [42] Zakaria ZA, et al. In vitro antimicrobial activity of Mutingia Calabura extracts and fractions. African Journal of Microbiology Research. 2010;4 (4):304-308. [43] Zanotto-Filho A. et al.The curry spice curcumin selectively inhibits cancer cells growth in vitro and in preclinical model of glioblastoma. The Journal of Nutritional Biochemistry. 2012; 23 (6): 591–601.
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12917 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12917 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal image/jpeg inches 758 96 96 norman 1421 56 56 2016-04-27 10:38:36 1421x758 7224-01-FH02-FSK-16-05740.jpg UniSZA Private Access Screening Of bismillah leaf (Vernonia Amygdalina) extraction for antiproliferative activies in human glioblastoma brain cancer cell lines Research Journal of Pharmaceutical, Biological and Chemical Sciences Vernonia amygdalina is a member of the Asteraceae family. It is a shrub or small tree that can reach up to 10 m in height and with a trunk diameter reaching up to 40 cm. The plant is a vegetable in its native land and is put through a detoxification process before it is cooked as food. The process involves boiling the leaves several times while changing the water after each cycle until the bitterness disappears. This study was carried out to screen the ethanol, methanol and ethyl acetate extracts of Vernonia Amygdalina as an anti-proliferative agent that inhibits the growth of human glioblastoma brain cancer cell lines (U-87). The anti-proliferative study indicated that only ethyl acetate extracts from Vernonia amygdalina were found to be cytotoxic towards U-87 cancer cell lines with an IC50 value of 18.80 ± 1.11 µg/ml. There was no IC50 value for ethanol and methanol extracts from Vernonia amygdalina. Thus, ethyl acetate extracts from Vernonia amygdalina could be envisaged for further exploration in efforts to develop newer and safer cytotoxic compounds. 7 2 Research Journal of Pharmaceutical, Biological and Chemical Sciences Research Journal of Pharmaceutical, Biological and Chemical Sciences 1084-1089 [1] Amarowicz, R., Naczk, M. et al. Antioxidant activity of condensed tannins of beach pea, canola hulls, evening primrose and Faba bean. Journal Food Lipids. 2000; 7: 195–205. [2] Arora-Kuruganti, P., Lucchesi, P. A. and Wurster, R. D. Proliferation of Cultured Human Astrocytoma Cells in Response to an Oxidant and Antioxidant. Journal of Neuro-Oncology. 1999;44 (3): 213–221. [3] Atangwho, I. J., Ebong, P. E., Egbung, G. E., Eteng, M. U. and Eyong, M. U. Effects of Vernonia amygdalina Del:leaf on liver function in alloxan induced hyperglycemic rats. J. Pharm. Bioresource. 2007c; 4(1): 1-7. [4] Atangwho, I. J., Ebong, P. E., Eyong, E. U., Eteng, M. U. and Obi, A. U. Effects of Vernonia amygdalina Del:leaf on kidney function of diabetic rats. Int. J. Pharm. 2007b; 3(2):143- 148. [5] Atangwho IJ, Ebong PO, et al. Vernonia amygdalina Del: A potential prophylactic antidiabetic agent in lipids complication. Glob. J Pure Applied Sci. 2007a; 3(1); 103-106. [6] Boeing, H., Bechthold, A., Bub, A. et al. Critical review: vegetables and fruit in the prevention of chronic diseases. European Journal of Nutrition. 2012; 51 (6): 637–663. [7] Buchanan DR, White JD, et al. Research-design issues in cancer-symptom-management trials using complementary and alternative medicine: lessons from the National Cancer Institute Community Clinical Oncology Program experience. Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology. 2005; 23 (27): 6682–9. [8] Chen, D., Pace, P. E., Coombes, R. C. and Ali, S. Phosphorylation of human estrogen receptor alpha by protein kinase A regulates dimerization. Molecular Cell Biology. 1999; 19 (2): 1002-15. [9] Duan, X., Wu, G. and Jiang, Y. Evaluation of the antioxidant properties of litchi fruit phenolics in relation to pericarp browning prevention. Molecules. 2007; 12 (4): 759-771. [10] Eleonora, T., Lorenzo, F. and Carmela, F. Potential effects of pomegranate polyphenols in cancer prevention and therapy. Oxidative medicine and cellular longevity. 2015:1-19. [11] Farombi, E. O. and Owoeye, O. Antioxidative and chemopreventive properties of Vernonia amygdalina and Garcinia biflavonoid. Int J Environ Res Public Health. 2011; 8(6): 2533-55. [12] Gawron, A. and Kruk, G. Cytotoxic effect of xanthotoxol 8-hydroxypsoralen on TCTC cells in vitro. Polish Journal of Pharmacology and Pharmacy. 1992; 44 (1): 51-57. [13] Geran, R. I., Greenberg, H. M., McDonald, M. and Abbott, B. J. Protocols for screening chemical agents and natural products against animal tumours and other biological systems. Cancer Chemoth Rep. 1972; 33: 1-17. [14] Gresham, L. J., Ross, J. and Izevbigie, E. B. Vernonia amygdalina; anticancer activity, authentication and adulteration detection. Int J Environ Res Public Health. 2008; 5(5): 342-8. [15] Grubben, G. J. H. Prota volume 2- vegetables, prota foundation. Wageningen. 2004: 543-544. [16] Harborne, J. B. Arsenal for Survival: Secondary Plant Products. Taxon. 2000; 49 (3): 435. [17] Kanatt, S. R., Chander, R. and Sharma, A. Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products. International Journal of Food Science & Technology. 2010; 45 (2): 216–222. [18] Khalili, M. A., Hadi, N. A., Naim, R., Baig, A. A. and Mahmud, K. Study on antioxidant capacity and anticancer activity of Bismillah leaf (Vernonia amygdalina). World Journal of Pharmaceutical Research. 2014; 3 (6): 14-29. [19] Khoshyomn, S., et al. Synergistic effect of genistein and BCNU on growth inhibition and cytotoxicity of glioblastoma cells. Journal of Neuro-Oncology. 2002; 57 (3): 193–200. [20] Li, H., Wang, X., Li, P., Li, Y. and Wang, H. Polyphenolics compounds and antioxidant properties of selected china wines. Food chemistry. 2009; 112 (2): 454-460. [21] Livingstone, J. Natural compounds in cancer therapy. International Journal Of Pharmaceutical Medicine. 2001; 15. [22] Lu, F. and Foo, L.Y. Toxicological aspects of food antioxidants. In: Madhavi DL, Deshpande SS, Salunkhe DK, editors. Food antioxidants. New York: Marcel Dekker. 1995. [23] Luo, X., Jiang, Y., Fronczek, F. R., Lin, C., Izevbigie, E. B. and Lee, K. S. Isolation and structure determination of a sesquiterpene lactone (vernodalinol) from Vernonia amygdalina extracts. Pharm boil. 2011; 49 (5): 464-70. [24] Luo, X., Oyugi, D. A., Lin, C., Izevbigie, E. B. and Lee, K. S. Isolation and characterization of the antibreast carcinoma cell growth components of Vernonia amygdalina extracts. Exp Boil Med (Maywood). 2010; 235 (12): 1472-8. [25] Mohd, Zin, Z., Abdul Hamid, A. and Osman, A. Antioxidative activity of extracts from mengkudu (Morinda citrifolia L.) root, fruit and leaf. Food. Chem. 2002; 78: 227-231. [26] Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983; 65 (1-2): 55–63. [27] Mohd Adzim Khalili Rohin, Norhayati Abd Hadi, Rochman Naim, Atif Amin Baig, Khairil Mahmud: Study On Antioxidant Capacity And Anticancer Activity Of Bismillah Leaf (Vernonia Amygdalina). World Journal of Pharmaceutical Research 09/2014; 3(6):14-29. [28] Mohd Adzim Khalili R, Che Abdullah A.B, And Abdul Manaf A: Total Antioxidant Activity, Total Phenolic Content And Radical Scavenging Activity Both Flesh And Peel Of Red Pitaya, White Pitaya And Papaya. International Journal of Pharmacy and Pharmaceutical Sciences 04/2012; Vol 4,(Issue 2):113-122. [29] Mohd Adzim Khalili R, Norhayati AH, et al. Determination of radical scavenging activity and Vitamin A, C and E in organically grown Red Pitaya (Hylocereus sp.). International Food Research Journal 06/2010; Volume 17.(Issue 2.-ISSN 22317546):405-409. [30] Nwaoguikpe, R. N. The effect of extract of bitter leaf (Vernonia amygdalina) on blood glucose levels of diabetic rats. Int. Journal Biology Chemical Science. 2010; 4 (3): 721-729. [31] Oyugi, D. A., Luo, X., Lee, K. S., Hill, B. and Izevbigie, E. B. Activity markers of the anti-breast carcinoma cell growth fractions of Vernonia amygdalina extracts. Exp Boil Med (Maywood). 2009; 234 (4): 410-7. [32] Pan, Y., Wang, K., Huang, S., et al. Antioxidant activity of micro-wave assisted extract of Longan (Dimocarpus longan lour.) peel. Food chemistry. 2008;106 (3): 1264-1270. [33] Phyllistin AB, James FB.. Tips for preventing food poisoning “Herbs” American No.1 Guide Natural health, 3rd edn. Publ. Averge, 2000; 9: 383-386. [34] Richardson MA, et al. Complementary/Alternative Medicine Use in a Comprehensive Cancer Center and the Implications for Oncology. J. Clin. Oncol.. 2000; 18 (13): 2505–2514. [35] Scheck, A. C., Perry, K., Hank, N. C. and Clark, W. D. Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells. BMC Complementary and Alternative Medicine. 2006; 6 (27). [36] Scheck, A. C., Shapiro, J. R., Coons, S. W., Norman, S. A. and Johnson, P. C. Biological and molecular analysis of a low-grade recurrence of a glioblastoma multiforme. Clinical Cancer Research : An Official Journal of the American Association for Cancer Research. 1996; 2 (1):187–99. [37] Schiffers, R.R. African indigenous vegetables: an overview of the cultivated species. University Greenwich Press, England. 2000. [38] Schwartsmann, G., Winograd, B. and Pinedo, H. M. The main steps in the development of anticancer agents. Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology. 1988;12 (4): 301–13. [39] Turrini, E., Ferruzzi, L. and Fimognari, C. Potential Effects of Pomegranate Polyphenols in Cancer Prevention and Therapy. 2015. [40] Yedjou, C., Izevbigie, E. and Tchounwou, P. Preclinical assessment of Vernonia amygdalina leaf extracts as DNA damaging anti-cancer agent in the management of breast cancer. Int J Environ Res Public Health. 2008; 5(5): 337-41. [41] Zakaria ZA, et al. In vitro antistaphylococcal activity of the extracts of several neglected plants in Malaysia. International Journal of Pharmacology. 2007; 3 (5): 428-431. [42] Zakaria ZA, et al. In vitro antimicrobial activity of Mutingia Calabura extracts and fractions. African Journal of Microbiology Research. 2010;4 (4):304-308. [43] Zanotto-Filho A. et al.The curry spice curcumin selectively inhibits cancer cells growth in vitro and in preclinical model of glioblastoma. The Journal of Nutritional Biochemistry. 2012; 23 (6): 591–601.
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Screening Of bismillah leaf (Vernonia Amygdalina) extraction for antiproliferative activies in human glioblastoma brain cancer cell lines
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| summary |
Vernonia amygdalina is a member of the Asteraceae family. It is a shrub or small tree that can reach up to 10 m in height and with a trunk diameter reaching up to 40 cm. The plant is a vegetable in its native land and is put through a detoxification process before it is cooked as food. The process involves boiling the leaves several times while changing the water after each cycle until the bitterness disappears. This study was carried out to screen the ethanol, methanol and ethyl acetate extracts of Vernonia Amygdalina as an anti-proliferative agent that inhibits the growth of human glioblastoma brain cancer cell lines (U-87). The anti-proliferative study indicated that only ethyl acetate extracts from Vernonia amygdalina were found to be cytotoxic towards U-87 cancer cell lines with an IC50 value of 18.80 ± 1.11 µg/ml. There was no IC50 value for ethanol and methanol extracts from Vernonia amygdalina. Thus, ethyl acetate extracts from Vernonia amygdalina could be envisaged for further exploration in efforts to develop newer and safer cytotoxic compounds.
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| title |
Screening Of bismillah leaf (Vernonia Amygdalina) extraction for antiproliferative activies in human glioblastoma brain cancer cell lines
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| title_full |
Screening Of bismillah leaf (Vernonia Amygdalina) extraction for antiproliferative activies in human glioblastoma brain cancer cell lines
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| title_fullStr |
Screening Of bismillah leaf (Vernonia Amygdalina) extraction for antiproliferative activies in human glioblastoma brain cancer cell lines
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| title_full_unstemmed |
Screening Of bismillah leaf (Vernonia Amygdalina) extraction for antiproliferative activies in human glioblastoma brain cancer cell lines
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| title_short |
Screening Of bismillah leaf (Vernonia Amygdalina) extraction for antiproliferative activies in human glioblastoma brain cancer cell lines
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| title_sort |
screening of bismillah leaf (vernonia amygdalina) extraction for antiproliferative activies in human glioblastoma brain cancer cell lines
|