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12868
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UniSZA
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[1] Epps SVR, Harvey RB, Hume ME, Phillips TD, Anderson RC, Nisbet DJ. Foodborne Campylobacter: infections, metabolism, pathogenesis and reservoirs. Int J Environ Res Public Health 2013; 10: 6292-304. [2] Whiley H, van den Akker B, Giglio S, Betham R. The role of environmental reservoirs in human Campylobacteriosis. Int J En- viron Res Public Health 2013; 10: 5886-907. [3] Mansouri-najand L, Saleha AA, Wai SS. Prevalence of multidrug resistance Campylobacter jejuni and Campylobacter coli in chickens slaughtered in selected markets, Malaysia. Trop Biomed 2012; 29: 231-8. [4] Chai LC, Fatimah AB, Ghazali FM, Lee HY, Tunung R, Shamsinar AT, et al. Biosafety of Campylobacter jejuni from raw vegetables consumed as Ulam with reference to their resistance to antibiotics. Int Food Res J 2008; 15: 125-34. [5] Krumperman PH. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamina- tion of foods. Appl Environ Microbiol 1983; 46: 165-70. [6] Yoo JH, Choi NY, Bae YM, Lee JS, Lee SY. Development of a selective agar plate for the detection of Campylobacter spp. in fresh produce. Int J Food Microbiol 2014; 189: 67-74. [7] Chai LC, Robin T, Ragavan UM, Gunsalam JW, Bakar FA, Ghazali FM, et al. Thermophilic Campylobacter spp. in salad vegetables in Malaysia. Int J Food Microbiol 2007; 117: 106-11. [8] Khalid MI, Tang JY, Baharuddin NH, Rahman NS, Rahimi NF, Radu S. Prevalence, antibiogram, and cdt genes of toxigenic Campylobacter jejuni in salad styled vegetables (ulam) at farms and retail outlets in Terengganu. J Food Prot 2015; 78: 65-71. [9] Chai LC, Ghazali FM, Bakar FA, Lee HY, Suhaimi LR, Talib SA, et al. Occurrence of thermophilic Campylobacter spp. on vegeta- bles farms in Malaysia. J Microbiol Biotechnol 2009; 19: 1415-20. [10] Wang G, Clark CG, Taylor TM, Pucknell C, Barton C, Price L, et al. Colony multiplex PCR assay for identification and differen- tiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J Clin Microbiol 2002; 40: 4744-7. [11] Clinical and Laboratory Standards Institute. Methods for antimi- crobial dilution and disk susceptibility testing of infrequently iso- lated or fastidious bacteria; approved guide. Line M45-A. Wayne: Clinical and Laboratory Standards Institute; 2006. [12] Ge B, Wang F, Sjӧlund-Karlsson M, McDermott PF. Antimicrobial resistance in Campylobacter: susceptibility testing methods and resistance trends. J Microbiol Methods 2013; 95: 57-67. [13] Keller J, Perreten V. Genetic diversity in fluoroquinolone and macrolide-resistant Campylobacter coli from pigs. Vet Microbiol 2006; 113: 103-8. [14] Andersen SR, Saadbye P, Shukri NM, Rosenquist H, Nielsen NL, Boel J. Antimicrobial resistance among Campylobacter jejuni isolated from raw poultry meat at retail level in Denmark. Int J Food Microbiol 2006; 107: 250-5. [15] Bae W, Kaya KN, Hancock DD, Call DR, Park YH, Besser TE, et al. Prevalence and antimicrobial resistance of thermophilic Campylobacter spp. from cattle farms in Washington State. Appl Environ Microbiol 2005; 71: 169-74. [16] Ge B, White DG, McDermott PF, Girard W, Zhao S, Hubert S, et al. Antimicrobial-resistant Campylobacter species from retail raw meats. Appl Environ Microbiol 2003; 69: 3005-7. [17] Wieczorek K, Denis E, Osek J. Comparative analysis of antimicro- bial resistance and genetic diversity of Campylobacter from broilers slaughtered in Poland. Int J Food Microbiol 2015; 210: 24-32. [18] Han K, Jang SS, Choo E, Heu S, Ryu S. Prevalence, genetic di- versity and antibiotic resistance patterns of Campylobacter jejuni from retail raw chicken in Korea. Int J Food Microbiol 2007; 114: 50-9. [19] Rodrigo S, Adesiyun A, Asgarali Z, Swanston W. Antimicrobial resistance of Campylobacter spp. isolated from broilers in small poultry processing operations in Trinidad. Food Control 2007; 18: 321-5. [20] Luo N, Pereira S, Sahin O, Lin J, Huang S, Michel L, et al. Enhanced in vivo fitness of fluoroquinolone-resistant Campylo- bacter jejuni in the absence of antibiotic selection pressure. Proc Natl Adad Sci U S A 2005; 102: 541-6. [21] Price LB, Johnson E, Vailes R, Silbergeld E. Fluoroquinolone- resistant Campylobacter isolates from conventional and antibiotic- free chicken products. Environ Health Perspect 2005; 113: 557-60. [22] Economou V, Zisides N, Gousia P, Petsios S, Sakkas H, Soultos N, et al. Prevalence and antimicrobial profile of Campylobacter iso- lates from free-range and conventional farming chicken meat during a 6-year survey. Food Control 2015; 56: 161-8. [23] Siddique FM, Akram M, Noureen N, Noreen Z, Bokhari H. Antibiotic susceptibility profiling and virulence potential of Campylobacter jejuni isolates from different sources in Pakistan. Asian Pac J Trop Med 2015; 8: 197-202. [24] Engberg J, Aarestrup FM, Taylor DE, Gerner-Smidt P, Nachamkin I. Quinolone and macrolide resistance in Campylo- bacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg Infect Dis 2001; 7(1): 24-34. [25] Gibreel A, Taylor DE. Macrolide resistance in Campylobacter jejuni and Campylobacter coli. J Antimicrob Chemother 2006; 58: 243-55. [26] Navarro F, Mir ́o E, Mirelis B, Prats G. Campylobacter spp. anti- biotic susceptibility. J Antimicrob Chemother 1993; 32: 906-7. [27] Sj ̈ogren E, Lindblom GB, Kaijser B. Norfloxacin resistance in Campylobacter jejuni and Campylobacter coli isolates from Swedish patients. J Antimicrob Chemother 1997; 40: 257-61. [28] Padungtod P, Kaneene JB, Hanson R, Morita Y, Boonmar S. Antimicrobial resistance in Campylobacter isolated from food an- imals and humans in northern Thailand. FEMS Immunol Med Microbiol 2006; 47: 217-25. [29] Garcia BC, Dimasupil MA, Vital PG, Widmer KW, Rivera WL. Fecal contamination in irrigation water and microbial quality of vegetable primary production in urban farms of Metro Manila, Philippines. J Environ Sci Health B 2015; 50: 734-43. [30] Munther D, Luo Y, Wu J, Magpantay FM, Srinivasan P. A mathematical model for pathogen cross-contamination dynamics during produce wash. Food Microbiol 2015; 51: 101-7. John Yew Huat Tang et al./Asian Pac J Trop Biomed 2016; 6(1): 71–75 75
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norman
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12868 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12868 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal image/jpeg inches 96 96 norman 37 37 1422 772 Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets 2016-02-17 09:34:43 1422x772 7175-01-FH02-FBIM-16-05358.jpg Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets UniSZA Private Access Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets Asian Pacific Journal of Tropical Biomedicine Objective: To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion method. RAPD analysis was done to characterize 98 C. jejuni isolates. Results: Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and below. This indicated that the isolates in the vegetables were not from the high risk environment or extensive farming practices. C. jejuni isolates found resistant towards penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), genta- mycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance to- wards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni in vegetables was likely due to cross contamination at retail markets. Conclusions: C. jejuni contamination in vegetables at retail markets was due to cross contamination. Current finding proved that C. jejuni in small scale vegetables production was less expose towards antibiotic abuse. 6 1 Hainan Medical University Hainan Medical University 71-75 [1] Epps SVR, Harvey RB, Hume ME, Phillips TD, Anderson RC, Nisbet DJ. Foodborne Campylobacter: infections, metabolism, pathogenesis and reservoirs. Int J Environ Res Public Health 2013; 10: 6292-304. [2] Whiley H, van den Akker B, Giglio S, Betham R. The role of environmental reservoirs in human Campylobacteriosis. Int J En- viron Res Public Health 2013; 10: 5886-907. [3] Mansouri-najand L, Saleha AA, Wai SS. Prevalence of multidrug resistance Campylobacter jejuni and Campylobacter coli in chickens slaughtered in selected markets, Malaysia. Trop Biomed 2012; 29: 231-8. [4] Chai LC, Fatimah AB, Ghazali FM, Lee HY, Tunung R, Shamsinar AT, et al. Biosafety of Campylobacter jejuni from raw vegetables consumed as Ulam with reference to their resistance to antibiotics. Int Food Res J 2008; 15: 125-34. [5] Krumperman PH. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamina- tion of foods. Appl Environ Microbiol 1983; 46: 165-70. [6] Yoo JH, Choi NY, Bae YM, Lee JS, Lee SY. Development of a selective agar plate for the detection of Campylobacter spp. in fresh produce. Int J Food Microbiol 2014; 189: 67-74. [7] Chai LC, Robin T, Ragavan UM, Gunsalam JW, Bakar FA, Ghazali FM, et al. Thermophilic Campylobacter spp. in salad vegetables in Malaysia. Int J Food Microbiol 2007; 117: 106-11. [8] Khalid MI, Tang JY, Baharuddin NH, Rahman NS, Rahimi NF, Radu S. Prevalence, antibiogram, and cdt genes of toxigenic Campylobacter jejuni in salad styled vegetables (ulam) at farms and retail outlets in Terengganu. J Food Prot 2015; 78: 65-71. [9] Chai LC, Ghazali FM, Bakar FA, Lee HY, Suhaimi LR, Talib SA, et al. Occurrence of thermophilic Campylobacter spp. on vegeta- bles farms in Malaysia. J Microbiol Biotechnol 2009; 19: 1415-20. [10] Wang G, Clark CG, Taylor TM, Pucknell C, Barton C, Price L, et al. Colony multiplex PCR assay for identification and differen- tiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J Clin Microbiol 2002; 40: 4744-7. [11] Clinical and Laboratory Standards Institute. Methods for antimi- crobial dilution and disk susceptibility testing of infrequently iso- lated or fastidious bacteria; approved guide. Line M45-A. Wayne: Clinical and Laboratory Standards Institute; 2006. [12] Ge B, Wang F, Sjӧlund-Karlsson M, McDermott PF. Antimicrobial resistance in Campylobacter: susceptibility testing methods and resistance trends. J Microbiol Methods 2013; 95: 57-67. [13] Keller J, Perreten V. Genetic diversity in fluoroquinolone and macrolide-resistant Campylobacter coli from pigs. Vet Microbiol 2006; 113: 103-8. [14] Andersen SR, Saadbye P, Shukri NM, Rosenquist H, Nielsen NL, Boel J. Antimicrobial resistance among Campylobacter jejuni isolated from raw poultry meat at retail level in Denmark. Int J Food Microbiol 2006; 107: 250-5. [15] Bae W, Kaya KN, Hancock DD, Call DR, Park YH, Besser TE, et al. Prevalence and antimicrobial resistance of thermophilic Campylobacter spp. from cattle farms in Washington State. Appl Environ Microbiol 2005; 71: 169-74. [16] Ge B, White DG, McDermott PF, Girard W, Zhao S, Hubert S, et al. Antimicrobial-resistant Campylobacter species from retail raw meats. Appl Environ Microbiol 2003; 69: 3005-7. [17] Wieczorek K, Denis E, Osek J. Comparative analysis of antimicro- bial resistance and genetic diversity of Campylobacter from broilers slaughtered in Poland. Int J Food Microbiol 2015; 210: 24-32. [18] Han K, Jang SS, Choo E, Heu S, Ryu S. Prevalence, genetic di- versity and antibiotic resistance patterns of Campylobacter jejuni from retail raw chicken in Korea. Int J Food Microbiol 2007; 114: 50-9. [19] Rodrigo S, Adesiyun A, Asgarali Z, Swanston W. Antimicrobial resistance of Campylobacter spp. isolated from broilers in small poultry processing operations in Trinidad. Food Control 2007; 18: 321-5. [20] Luo N, Pereira S, Sahin O, Lin J, Huang S, Michel L, et al. Enhanced in vivo fitness of fluoroquinolone-resistant Campylo- bacter jejuni in the absence of antibiotic selection pressure. Proc Natl Adad Sci U S A 2005; 102: 541-6. [21] Price LB, Johnson E, Vailes R, Silbergeld E. Fluoroquinolone- resistant Campylobacter isolates from conventional and antibiotic- free chicken products. Environ Health Perspect 2005; 113: 557-60. [22] Economou V, Zisides N, Gousia P, Petsios S, Sakkas H, Soultos N, et al. Prevalence and antimicrobial profile of Campylobacter iso- lates from free-range and conventional farming chicken meat during a 6-year survey. Food Control 2015; 56: 161-8. [23] Siddique FM, Akram M, Noureen N, Noreen Z, Bokhari H. Antibiotic susceptibility profiling and virulence potential of Campylobacter jejuni isolates from different sources in Pakistan. Asian Pac J Trop Med 2015; 8: 197-202. [24] Engberg J, Aarestrup FM, Taylor DE, Gerner-Smidt P, Nachamkin I. Quinolone and macrolide resistance in Campylo- bacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg Infect Dis 2001; 7(1): 24-34. [25] Gibreel A, Taylor DE. Macrolide resistance in Campylobacter jejuni and Campylobacter coli. J Antimicrob Chemother 2006; 58: 243-55. [26] Navarro F, Mir ́o E, Mirelis B, Prats G. Campylobacter spp. anti- biotic susceptibility. J Antimicrob Chemother 1993; 32: 906-7. [27] Sj ̈ogren E, Lindblom GB, Kaijser B. Norfloxacin resistance in Campylobacter jejuni and Campylobacter coli isolates from Swedish patients. J Antimicrob Chemother 1997; 40: 257-61. [28] Padungtod P, Kaneene JB, Hanson R, Morita Y, Boonmar S. Antimicrobial resistance in Campylobacter isolated from food an- imals and humans in northern Thailand. FEMS Immunol Med Microbiol 2006; 47: 217-25. [29] Garcia BC, Dimasupil MA, Vital PG, Widmer KW, Rivera WL. Fecal contamination in irrigation water and microbial quality of vegetable primary production in urban farms of Metro Manila, Philippines. J Environ Sci Health B 2015; 50: 734-43. [30] Munther D, Luo Y, Wu J, Magpantay FM, Srinivasan P. A mathematical model for pathogen cross-contamination dynamics during produce wash. Food Microbiol 2015; 51: 101-7. John Yew Huat Tang et al./Asian Pac J Trop Biomed 2016; 6(1): 71–75 75
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| spellingShingle |
Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
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| summary |
Objective: To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion method. RAPD analysis was done to characterize 98 C. jejuni isolates. Results: Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and below. This indicated that the isolates in the vegetables were not from the high risk environment or extensive farming practices. C. jejuni isolates found resistant towards penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), genta- mycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance to- wards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni in vegetables was likely due to cross contamination at retail markets. Conclusions: C. jejuni contamination in vegetables at retail markets was due to cross contamination. Current finding proved that C. jejuni in small scale vegetables production was less expose towards antibiotic abuse.
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| title |
Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
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| title_full |
Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
|
| title_fullStr |
Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
|
| title_full_unstemmed |
Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
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| title_short |
Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
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| title_sort |
antibiotic resistance profile and rapd analysis of campylobacter jejuni isolated from vegetables farms and retail markets
|