Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a Malaysian Aaptos aaptos

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internalnotes [1] EL Larghi; ML Bohn; TS Kaufman. Tetrahedron, 2009, 65, 4257-4282. [2] H Nakamura; J Kobayashi; Y Ohizumi. Tetrahedron Lett., 1982, 23, 5555-5558. [3] A Rudi; Y Kashman. Tetrahedron Lett., 1993, 34, 4683-4684. [4] WF Tinto. Heterocycles, 1998, 48, 2089-2093. [5] AF Coutinho; B Chanas; TML e Souza; ICPP Frugrulhetti; RdA Epifanio. Heterocycles, 2002, 57, 1265-1272. [6] A Herlt; L Mander; W Rombang; R Rumampuk; S Soemitro; W Steglich; P Tarigan; FV Nussbaum. Tetrahedron, 2004, 60, 6101-6104. [7] S Takamatsu; TW Hodges; I Rajbhandari; WH Gerwick; MT Hamann; DG Nagle. J.Nat.Prod., 2003, 66, 605- 608. [8] S Aoki; D Kong; H Suna; Y Sowa; T Sakai; A Setiawan; M Kobayashi. Biochem and Biophysic. Res. Comm., 2006, 342, 101-106. [9] JA Diers; JJ Bowling; SO Duke; S Wahyuono; M Kelly; MT Hamann. Mar. Biotechnol., 2006, 8, 366-372. [10] W Gul; NL Hammond; M Yousaf; JJ Bowling; RF Schinazi; SS Wirtz; GdC Andrews; C Cuevas; MT Hamann. Bioorg.Med.Chem., 2006, 14, 8495-8505. [11] JJ Bowling; HK Pennaka; K Ivey; S Wahyuono; Kelly M; Schinazi RF; Valeriote FA; Graves DE; Hamann MT. Abst. Chem. Biol. Drug Des., 2007, 71, 205-215. [12] JA Diers; KD Ivey; A El-Alfy; J Shaikh; J Wang; AJ Kochanowska; JF Stoker; MT Hamann; RR Matsumoto. Pharmacol. Biochem.Behav., 2008, 89, 46-53. [13]M Habsah; ZM Rashid; F Nurhana; SM Khamsah; S Jasnizat; AM Ali; MN Lajis; F Shaharom; MH Zain. In Proceeding of Natural Product Malaysia 2005 the Twenty First Annual Seminar, Putrajaya, Malaysia, 2005, November 22-23. [14] S Khozirah; KC Ling; ZM Rashid; TP Jean; A Faridah; MR Salahudin; Z Zurina; NH Lajis; M Habsah; AM Ali. Mar. Drugs, 2009,7, 1-8. [15] M Habsah; ZM Rashid; S Khozirah; L Jalifah; MN Lajis; AM Ali. Pertanika J. Trop. Agric. Sci., 2009, 32 (1), 43-50. [16] WC Earnshaw. Trends in cell biology, 1995, 5 (6), 217-220. [17] G Majno; I Joris. Review. Am.J.Pathol., 1995, 146 (1), 3-15. [18] A Ishaque, M Al-rubeai. Monitoring of Apoptosis. In: Cell Engineering IV, 1st edition, Kluwer Academic Publishers, Netherlands, 2004, 281-306. [19] T Mosmann. J.Immunol.Methods, 1983, 65, 55-63. [20] ZM Rashid; E Lahaye; D Defer; P Douzenel; B Perrin; N Bourgougnon; O Sire. Int.J.Biol.Macromol., 2009, 44, 286-293. [21] Y Andriani; AWM Effendy; TMT Sifzizul; M Habsah. Int. J. Pharm. Res. 2011, 2, 1700–1706. [22] Y Andriani; TS Tengku Muhammad; H Mohamad; DF Syamsumir; J Saidinl; GS Chew; AWM Effendy. Molecules, 2015, 20, 4410-4429. [23] L Ding; B Liu; L-I Qi; Q-Y Zhou; Q Hou; J Li; Q Zhang. Toxicol.in Vitro, 2009, xxx, 1-10. [24] HI Bank. In Vitro Cell. Dev.Biol., 1988, 24 (4), 266-273. [25] AA Nanji; S Hiller-Sturmhöfel. Alcohol Health Res.World , 1997, 21, 325-330. [26] MD Lovelace; DM Cahill. J.Neurosci.Methods, 165, 2007, 223-229. [27] Q He & Q Na. Leuk.Res., 2001, 25, 455-461. [28] RA Fleck; S Romero-Steiner; MH Nahm. Clin.Diagn.Lab.Immun., 2005, 12 (1), 19-27. [29] RF Martins; MF Ramos; L Herfindal; JA Sousa; K Skarven; VM Vasconcelos. Mar. Drugs, 2008, 6 (1), 1-11. [30] AH Wyllie; JFR Kerr; AR Currie. Int.Rev.Cytol.,1980, 68, 251-306. [31] AH Wyllie. Cell death-Apoptosis and necrosis. In: Apoptosis and cell proliferation, 2nd edition. Boehringer, Mannheim, 1998, 2-61. [32] MB Hampton & S Orrenius. FEBS Letters, 1997, 414, 552-556. [33] JF Curtin; M Donovan; TG Cotter. J.Immunol.Methods, 2002, 265, 49-72. [34] J Li; W Li; J Su; W Liu; BT Altura; BM Altura. Brain Res.Bull., 2003, 62, 101-106. [35] H Nakamura; J Kobayashi; Y Ohizumi. J. Chem. Soc., Perkin Trans., 1987, 1, 173-176.
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person Hamendra ji
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spelling 12769 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12769 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf Adobe Acrobat Pro DC 20 Paper Capture Plug-in with ClearScan 13 1.6 Hamendra ji 2024-08-27 13:54:14 7076-01-FH02-FBIM-16-05500.pdf UniSZA Private Access Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a Malaysian Aaptos aaptos Journal of Chemical and Pharmaceutical Research The present study was investigated the cytotoxic activity of a new aaptaminoid of A. aaptos against human myeloid leukemia cell line HL-60 and murine myelomonocytic leukemia cell line WEHI-3B, as well as the mode of cells death and antiviral activity against Herpes simplex virus type 1 HSV-1, infected normal mammalian cells, Cercopithecus aethiops African green monkey kidney cell line VERO. Cell viability was evaluated by Methyl Thiazole Tetrazolium (MTT) assay, following the induction of apoptosis assessment by light microscopy and fluorescence microscopy (Acridine orange/ Propidium iodide (AO/PI) double staining analysis) while using hydrogen peroxide (H2O2) as a standard. The anti-HSV-1 activity was evaluated using neutral red uptake assay. A new alkaloid 3-(phenethylamino)demethyl(oxy)aaptamine(8-methoxy-2-(phenethylamino)-9H-benzo[d,e][1,6] naphthyridin-9-one) (1) and aaptamine (8,9-dimethoxy-1H-benzo[d,e][1,6]-naphthyridin) (2) strongly reduced HL60 viability while also showing cytotoxicity against WEHI-3B, in which the mode of both cells death was through induction of apoptosis. In anti-herpetic investigation against HSV-1, only compound 2 showed strong anti-HSV-1 activity. The compounds however did not exert significant cytotoxic effect on VERO, confirming their tumour-selective cytotoxicity. Result concluded that compounds 1 and 2 produced a good cytotoxicity activity on HL-60 and WEHI-3B. Both compounds inhibited the cells via apoptotic cell death mechanism. In addition, compound 2 also exhibited a good antivirus activity on HSV-1. 7 10 330-341 [1] EL Larghi; ML Bohn; TS Kaufman. Tetrahedron, 2009, 65, 4257-4282. [2] H Nakamura; J Kobayashi; Y Ohizumi. Tetrahedron Lett., 1982, 23, 5555-5558. [3] A Rudi; Y Kashman. Tetrahedron Lett., 1993, 34, 4683-4684. [4] WF Tinto. Heterocycles, 1998, 48, 2089-2093. [5] AF Coutinho; B Chanas; TML e Souza; ICPP Frugrulhetti; RdA Epifanio. Heterocycles, 2002, 57, 1265-1272. [6] A Herlt; L Mander; W Rombang; R Rumampuk; S Soemitro; W Steglich; P Tarigan; FV Nussbaum. Tetrahedron, 2004, 60, 6101-6104. [7] S Takamatsu; TW Hodges; I Rajbhandari; WH Gerwick; MT Hamann; DG Nagle. J.Nat.Prod., 2003, 66, 605- 608. [8] S Aoki; D Kong; H Suna; Y Sowa; T Sakai; A Setiawan; M Kobayashi. Biochem and Biophysic. Res. Comm., 2006, 342, 101-106. [9] JA Diers; JJ Bowling; SO Duke; S Wahyuono; M Kelly; MT Hamann. Mar. Biotechnol., 2006, 8, 366-372. [10] W Gul; NL Hammond; M Yousaf; JJ Bowling; RF Schinazi; SS Wirtz; GdC Andrews; C Cuevas; MT Hamann. Bioorg.Med.Chem., 2006, 14, 8495-8505. [11] JJ Bowling; HK Pennaka; K Ivey; S Wahyuono; Kelly M; Schinazi RF; Valeriote FA; Graves DE; Hamann MT. Abst. Chem. Biol. Drug Des., 2007, 71, 205-215. [12] JA Diers; KD Ivey; A El-Alfy; J Shaikh; J Wang; AJ Kochanowska; JF Stoker; MT Hamann; RR Matsumoto. Pharmacol. Biochem.Behav., 2008, 89, 46-53. [13]M Habsah; ZM Rashid; F Nurhana; SM Khamsah; S Jasnizat; AM Ali; MN Lajis; F Shaharom; MH Zain. In Proceeding of Natural Product Malaysia 2005 the Twenty First Annual Seminar, Putrajaya, Malaysia, 2005, November 22-23. [14] S Khozirah; KC Ling; ZM Rashid; TP Jean; A Faridah; MR Salahudin; Z Zurina; NH Lajis; M Habsah; AM Ali. Mar. Drugs, 2009,7, 1-8. [15] M Habsah; ZM Rashid; S Khozirah; L Jalifah; MN Lajis; AM Ali. Pertanika J. Trop. Agric. Sci., 2009, 32 (1), 43-50. [16] WC Earnshaw. Trends in cell biology, 1995, 5 (6), 217-220. [17] G Majno; I Joris. Review. Am.J.Pathol., 1995, 146 (1), 3-15. [18] A Ishaque, M Al-rubeai. Monitoring of Apoptosis. In: Cell Engineering IV, 1st edition, Kluwer Academic Publishers, Netherlands, 2004, 281-306. [19] T Mosmann. J.Immunol.Methods, 1983, 65, 55-63. [20] ZM Rashid; E Lahaye; D Defer; P Douzenel; B Perrin; N Bourgougnon; O Sire. Int.J.Biol.Macromol., 2009, 44, 286-293. [21] Y Andriani; AWM Effendy; TMT Sifzizul; M Habsah. Int. J. Pharm. Res. 2011, 2, 1700–1706. [22] Y Andriani; TS Tengku Muhammad; H Mohamad; DF Syamsumir; J Saidinl; GS Chew; AWM Effendy. Molecules, 2015, 20, 4410-4429. [23] L Ding; B Liu; L-I Qi; Q-Y Zhou; Q Hou; J Li; Q Zhang. Toxicol.in Vitro, 2009, xxx, 1-10. [24] HI Bank. In Vitro Cell. Dev.Biol., 1988, 24 (4), 266-273. [25] AA Nanji; S Hiller-Sturmhöfel. Alcohol Health Res.World , 1997, 21, 325-330. [26] MD Lovelace; DM Cahill. J.Neurosci.Methods, 165, 2007, 223-229. [27] Q He & Q Na. Leuk.Res., 2001, 25, 455-461. [28] RA Fleck; S Romero-Steiner; MH Nahm. Clin.Diagn.Lab.Immun., 2005, 12 (1), 19-27. [29] RF Martins; MF Ramos; L Herfindal; JA Sousa; K Skarven; VM Vasconcelos. Mar. Drugs, 2008, 6 (1), 1-11. [30] AH Wyllie; JFR Kerr; AR Currie. Int.Rev.Cytol.,1980, 68, 251-306. [31] AH Wyllie. Cell death-Apoptosis and necrosis. In: Apoptosis and cell proliferation, 2nd edition. Boehringer, Mannheim, 1998, 2-61. [32] MB Hampton & S Orrenius. FEBS Letters, 1997, 414, 552-556. [33] JF Curtin; M Donovan; TG Cotter. J.Immunol.Methods, 2002, 265, 49-72. [34] J Li; W Li; J Su; W Liu; BT Altura; BM Altura. Brain Res.Bull., 2003, 62, 101-106. [35] H Nakamura; J Kobayashi; Y Ohizumi. J. Chem. Soc., Perkin Trans., 1987, 1, 173-176.
spellingShingle Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a Malaysian Aaptos aaptos
summary The present study was investigated the cytotoxic activity of a new aaptaminoid of A. aaptos against human myeloid leukemia cell line HL-60 and murine myelomonocytic leukemia cell line WEHI-3B, as well as the mode of cells death and antiviral activity against Herpes simplex virus type 1 HSV-1, infected normal mammalian cells, Cercopithecus aethiops African green monkey kidney cell line VERO. Cell viability was evaluated by Methyl Thiazole Tetrazolium (MTT) assay, following the induction of apoptosis assessment by light microscopy and fluorescence microscopy (Acridine orange/ Propidium iodide (AO/PI) double staining analysis) while using hydrogen peroxide (H2O2) as a standard. The anti-HSV-1 activity was evaluated using neutral red uptake assay. A new alkaloid 3-(phenethylamino)demethyl(oxy)aaptamine(8-methoxy-2-(phenethylamino)-9H-benzo[d,e][1,6] naphthyridin-9-one) (1) and aaptamine (8,9-dimethoxy-1H-benzo[d,e][1,6]-naphthyridin) (2) strongly reduced HL60 viability while also showing cytotoxicity against WEHI-3B, in which the mode of both cells death was through induction of apoptosis. In anti-herpetic investigation against HSV-1, only compound 2 showed strong anti-HSV-1 activity. The compounds however did not exert significant cytotoxic effect on VERO, confirming their tumour-selective cytotoxicity. Result concluded that compounds 1 and 2 produced a good cytotoxicity activity on HL-60 and WEHI-3B. Both compounds inhibited the cells via apoptotic cell death mechanism. In addition, compound 2 also exhibited a good antivirus activity on HSV-1.
title Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a Malaysian Aaptos aaptos
title_full Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a Malaysian Aaptos aaptos
title_fullStr Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a Malaysian Aaptos aaptos
title_full_unstemmed Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a Malaysian Aaptos aaptos
title_short Induction of apoptosis and anti HSV-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a Malaysian Aaptos aaptos
title_sort induction of apoptosis and anti hsv-1 activity of 3-(phenethylamino)demethyl(oxy)aaptamine from a malaysian aaptos aaptos