Characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics
| Format: | Restricted Document |
|---|
| _version_ | 1860797428350844928 |
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| building | INTELEK Repository |
| caption | Journal of Engineering and Applied Sciences |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 |
| date | 2020-05-04 10:31:38 |
| format | Restricted Document |
| id | 12677 |
| institution | UniSZA |
| originalfilename | 6984-01-FH02-FBIM-20-39452.pdf |
| person | N.A. Zulkifli M. Abd. Ghani and S. Abdul Mutalib |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12677 |
| spelling | 12677 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12677 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 8 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in Journal of Engineering and Applied Sciences N.A. Zulkifli M. Abd. Ghani and S. Abdul Mutalib 2020-05-04 10:31:38 Yeast strains local fruits and plant parts PCR-RFLP probiotic prebiotic Yeast strains local fruits and plant parts PCR-RFLP probiotic prebiotic 6984-01-FH02-FBIM-20-39452.pdf UniSZA Private Access Characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics Journal of Engineering and Applied Sciences Thirteen (n = 13) yeast strains isolated from different local fruits and plant parts were characterised by polymerase chain reaction–restriction fragment length polymorphism analysis. By using four restriction endonucleases (AluI, Hae-III, Hind III and HinfI) on amplicon-Targetedinternal Transcribed Spacer 1 (ITS1) and ITS2 regions, all yeast strains (n =13) were found to be Saccharomycessensuscripto group with Hae III which was the best limiting enzyme to differentiate between S. cerevisiae/S. paradoxus (325, 230, 170 and 125 bp) and S. bayanus/S. postrianus (495, 230 and 125 bp). Based on 5.8s rRNA gene, six yeast strains were identified as Saccharomyces cerevisiae, three strains were identified as S. paradoxus, and two strains were identified as S. bayanus and S. postrianus each. Probiotic test was conducted on 13 yeast strains which were grown on bile salt with concentrations from 0.3 to 1%. All strains were also examined for bile deconjugation, lysozyme tolerance and ability to utilise prebiotics. All yeast strains grew in bile salt at 0.3% concentration. However, only S. cerevisiaevar WU-Y2 (SM16) survived in 1% concentration. In bile deconjugation test, all yeast strains demonstrated reduction in growth and low tolerance towards high concentration of lysozyme (100 ppm). The yeast strains also revealed low growth in inulin, xylitol and lactulose (2% v/w). 15 9 2083-2090 |
| spellingShingle | Characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics |
| subject | Yeast strains local fruits and plant parts PCR-RFLP probiotic prebiotic |
| summary | Thirteen (n = 13) yeast strains isolated from different local fruits and plant parts were characterised by polymerase chain reaction–restriction fragment length polymorphism analysis. By using four restriction endonucleases (AluI, Hae-III, Hind III and HinfI) on amplicon-Targetedinternal Transcribed Spacer 1 (ITS1) and ITS2 regions, all yeast strains (n =13) were found to be Saccharomycessensuscripto group with Hae III which was the best limiting enzyme to differentiate between S. cerevisiae/S. paradoxus (325, 230, 170 and 125 bp) and S. bayanus/S. postrianus (495, 230 and 125 bp). Based on 5.8s rRNA gene, six yeast strains were identified as Saccharomyces cerevisiae, three strains were identified as S. paradoxus, and two strains were identified as S. bayanus and S. postrianus each. Probiotic test was conducted on 13 yeast strains which were grown on bile salt with concentrations from 0.3 to 1%. All strains were also examined for bile deconjugation, lysozyme tolerance and ability to utilise prebiotics. All yeast strains grew in bile salt at 0.3% concentration. However, only S. cerevisiaevar WU-Y2 (SM16) survived in 1% concentration. In bile deconjugation test, all yeast strains demonstrated reduction in growth and low tolerance towards high concentration of lysozyme (100 ppm). The yeast strains also revealed low growth in inulin, xylitol and lactulose (2% v/w). |
| title | Characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics |
| title_full | Characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics |
| title_fullStr | Characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics |
| title_full_unstemmed | Characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics |
| title_short | Characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics |
| title_sort | characterisation of yeasts isolated from different local fruits and plant parts and their potential as probiotics |