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1860797397883420672
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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| date |
2024-08-27 12:22:12
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Restricted Document
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| id |
12553
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UniSZA
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| internalnotes |
1. Romsing, J., Moiniche, S., Dahl, J.B. 2002. Rectal and parenteral paracetamol, and paracetamol in combination with NSAIDs, for postoperative analgesia. British Journal of Anaesthesia. 88(2): 215-256. 2. Boutaud, O., Aronoff D.M., Richardson, J.H., Marnett, L.J., Oates, J.A. 2002. Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H2 synthases. ProcNatlAcad Sci. 99 (10): 7130-7135. 3. Raffa, R.B., Walker, E.A., Sterious, S.N. 2004. Opioid receptors and acetaminophen (paracetamol). European Journal of Pharmacology. 503: 209– 210. 4. Dubois, R.N., Abramson, S.B., Crofford, L., Gupta, R.A., Simon, L.S., Van De Putte, L.B.A., Lipsky, P.E. 1998. Cyclooxygenase in biology and disease. FASEB. 12: 1063-1073. 5. Mirochnitchenko, O., Weisbrot-Lefkowitz, M., Reuhl, K., Cheni, L., Yangi, C., Inouye, M. 1999. Acetaminophen toxicity: opposite effects of two forms of gluthathione peroxidase. The Journal of Biological Chemistry. 274 (15): 10349–10355. 6. Narongchai, P. and Narongchai, S. 2004. Paracetamol overdose in suicidal attempt patients. J Med Assoc Thai. 87(4): 423-426. 7. Gujral, S.J, Knight, T.R., Farhood, A., Bajt, M.L., Jaeschke, H. 2002. Mode of cell death after acetaminophen overdose in mice: apoptosis or oncotic necrosis? Toxicological Sciences. 67: 322- 328. 8. Lorz, C., Justo, P., Sanz, A., Subira’, D., Egido, J., Ortiz, A. 2004. Paracetamol-induced renal tubular injury: a role for ER stress. J Am SocNephrol 15: 380–389. 9. Burkittet al. 2000. Wheather’s Basic Histopathology: A Color Atlas and Text. 10. Laskin, D.L., Laskin, J.D., 2001. Role of macrophages and inflammatory mediators in chemically induced toxicity. Toxicology 160, 111-118. 11. Jaeschke, H., Gores, G.J., Cederbaum, A.I., Hinson, J.A., Pessayre, D., Lemasters, J.J. 2002. Mechanisms of Hepatotoxicity. Toxicology Sciences. 65: 166-176. 12. Jaeschke H., Ho Y.S., Fisher M.A., Lawson J.A., Farhood A. 1999. Glutathione peroxidase deficient mice are more succeptible to neutrophil-mediated hepatic parenchymal cell injury during endotoxemia; importance of an intracellular oxidant stress. Hepatology 29: 443-450. 13. Lawson, J.A., Farhood, A., Hopper, R.D., Bajt, M.L., Jaeschke, H. 2000. The hepatic inflammatory response after acetaminophen overdose: role of neutrophils. Toxicological Sciences 54:509– 516. 14. Gopinath, C. 1989. Hepatic lesions in rodent toxicity studies. Proceeding of the 2nd Japanese Symposium on current perspectives in toxicology. 28-30 November 1989. Osaka and Tokyo. 15. Stevens, A., Lowe, J.S., Young, B. 2002. Cellular responses to injury. Wheather’s Basic Histopathology 4: 2-9. 16. Jaeschke, H. 2000. Reactive oxygen and mechanisms of inflammatory liver injury. J. Gastroenterol. Hepatol. 15: 718-724.
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6860-01-FH02-FSK-15-04244.pdf
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Adobe Acrobat Pro DC 20.6.20042
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12553
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| spelling |
12553 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12553 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf Adobe Acrobat Pro DC 20 Paper Capture Plug-in with ClearScan 8 1.6 Adobe Acrobat Pro DC 20.6.20042 2024-08-27 12:22:12 6860-01-FH02-FSK-15-04244.pdf UniSZA Private Access Paracetamol Induced Liver Morphological Changes after Acute Dosing Research Journal of Pharmacy and Technology Paracetamol, N-acetyl-p-aminophenol (APAP) belongs to a class of drugs called analgesics and antipyretics. It is considered a safe drug and available Over-The-Counter in most countries. This study was conducted to observe the effect of paracetamol on the liver of male Sprague Dawley rats. 30 rats were randomly divided into groups of 100mg/kg and 200mg/kg paracetamol and the control group was given saline. Treatment was given at a single dose of 1.0 ml/rat by intraperitoneal route. 3 rats were treated as normal as no treatment was given. 3 rats from each group were sacrificed after 24, 48 and 72 hours post-treatment. The liver section was stained with H and E staining and viewed under light microscope for lesion scoring. The statistical test shows that there were no significant decrease (p<0.05) in the mean weight of rats treated with paracetamol compared to saline-treated rats. The mean ratio of liver/100g body weight from rats treated with 100mg/kg and 200mg/kg paracetamol showed significant increase compared to saline-treated rats. Histological observation showed mild to moderate portal inflammation with infiltration and severe hyperemia in the 100mg/kg of paracetamol group. Liver section from rats administered with 200mg/kg paracetamol showed severe portal infiltration with hepatocytes undergoing necrosis and severe hyperemia. 200mg/kg paracetamol induced greater degree of necrosis compared to saline-treated group. The finding of this study suggested that even though paracetamol is considered a safe drug with low reported adverse effect, at higher dose (100-200mg/kg), there were significance morphological changes to the liver. 8 4 382-388 1. Romsing, J., Moiniche, S., Dahl, J.B. 2002. Rectal and parenteral paracetamol, and paracetamol in combination with NSAIDs, for postoperative analgesia. British Journal of Anaesthesia. 88(2): 215-256. 2. Boutaud, O., Aronoff D.M., Richardson, J.H., Marnett, L.J., Oates, J.A. 2002. Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H2 synthases. ProcNatlAcad Sci. 99 (10): 7130-7135. 3. Raffa, R.B., Walker, E.A., Sterious, S.N. 2004. Opioid receptors and acetaminophen (paracetamol). European Journal of Pharmacology. 503: 209– 210. 4. Dubois, R.N., Abramson, S.B., Crofford, L., Gupta, R.A., Simon, L.S., Van De Putte, L.B.A., Lipsky, P.E. 1998. Cyclooxygenase in biology and disease. FASEB. 12: 1063-1073. 5. Mirochnitchenko, O., Weisbrot-Lefkowitz, M., Reuhl, K., Cheni, L., Yangi, C., Inouye, M. 1999. Acetaminophen toxicity: opposite effects of two forms of gluthathione peroxidase. The Journal of Biological Chemistry. 274 (15): 10349–10355. 6. Narongchai, P. and Narongchai, S. 2004. Paracetamol overdose in suicidal attempt patients. J Med Assoc Thai. 87(4): 423-426. 7. Gujral, S.J, Knight, T.R., Farhood, A., Bajt, M.L., Jaeschke, H. 2002. Mode of cell death after acetaminophen overdose in mice: apoptosis or oncotic necrosis? Toxicological Sciences. 67: 322- 328. 8. Lorz, C., Justo, P., Sanz, A., Subira’, D., Egido, J., Ortiz, A. 2004. Paracetamol-induced renal tubular injury: a role for ER stress. J Am SocNephrol 15: 380–389. 9. Burkittet al. 2000. Wheather’s Basic Histopathology: A Color Atlas and Text. 10. Laskin, D.L., Laskin, J.D., 2001. Role of macrophages and inflammatory mediators in chemically induced toxicity. Toxicology 160, 111-118. 11. Jaeschke, H., Gores, G.J., Cederbaum, A.I., Hinson, J.A., Pessayre, D., Lemasters, J.J. 2002. Mechanisms of Hepatotoxicity. Toxicology Sciences. 65: 166-176. 12. Jaeschke H., Ho Y.S., Fisher M.A., Lawson J.A., Farhood A. 1999. Glutathione peroxidase deficient mice are more succeptible to neutrophil-mediated hepatic parenchymal cell injury during endotoxemia; importance of an intracellular oxidant stress. Hepatology 29: 443-450. 13. Lawson, J.A., Farhood, A., Hopper, R.D., Bajt, M.L., Jaeschke, H. 2000. The hepatic inflammatory response after acetaminophen overdose: role of neutrophils. Toxicological Sciences 54:509– 516. 14. Gopinath, C. 1989. Hepatic lesions in rodent toxicity studies. Proceeding of the 2nd Japanese Symposium on current perspectives in toxicology. 28-30 November 1989. Osaka and Tokyo. 15. Stevens, A., Lowe, J.S., Young, B. 2002. Cellular responses to injury. Wheather’s Basic Histopathology 4: 2-9. 16. Jaeschke, H. 2000. Reactive oxygen and mechanisms of inflammatory liver injury. J. Gastroenterol. Hepatol. 15: 718-724.
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| spellingShingle |
Paracetamol Induced Liver Morphological Changes after Acute Dosing
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| summary |
Paracetamol, N-acetyl-p-aminophenol (APAP) belongs to a class of drugs called analgesics and antipyretics. It is considered a safe drug and available Over-The-Counter in most countries. This study was conducted to observe the effect of paracetamol on the liver of male Sprague Dawley rats. 30 rats were randomly divided into groups of 100mg/kg and 200mg/kg paracetamol and the control group was given saline. Treatment was given at a single dose of 1.0 ml/rat by intraperitoneal route. 3 rats were treated as normal as no treatment was given. 3 rats from each group were sacrificed after 24, 48 and 72 hours post-treatment. The liver section was stained with H and E staining and viewed under light microscope for lesion scoring. The statistical test shows that there were no significant decrease (p<0.05) in the mean weight of rats treated with paracetamol compared to saline-treated rats. The mean ratio of liver/100g body weight from rats treated with 100mg/kg and 200mg/kg paracetamol showed significant increase compared to saline-treated rats. Histological observation showed mild to moderate portal inflammation with infiltration and severe hyperemia in the 100mg/kg of paracetamol group. Liver section from rats administered with 200mg/kg paracetamol showed severe portal infiltration with hepatocytes undergoing necrosis and severe hyperemia. 200mg/kg paracetamol induced greater degree of necrosis compared to saline-treated group. The finding of this study suggested that even though paracetamol is considered a safe drug with low reported adverse effect, at higher dose (100-200mg/kg), there were significance morphological changes to the liver.
|
| title |
Paracetamol Induced Liver Morphological Changes after Acute Dosing
|
| title_full |
Paracetamol Induced Liver Morphological Changes after Acute Dosing
|
| title_fullStr |
Paracetamol Induced Liver Morphological Changes after Acute Dosing
|
| title_full_unstemmed |
Paracetamol Induced Liver Morphological Changes after Acute Dosing
|
| title_short |
Paracetamol Induced Liver Morphological Changes after Acute Dosing
|
| title_sort |
paracetamol induced liver morphological changes after acute dosing
|