Isolation and antibiogram of Acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from Lahore, Pakistan

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date 2019-11-28 12:48:14
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id 12335
institution UniSZA
originalfilename 6635-01-FH02-FP-19-35978.pdf
person M. A. Razvy
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resourceurl https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12335
spelling 12335 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12335 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 7 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in M. A. Razvy 2019-11-28 12:48:14 6635-01-FH02-FP-19-35978.pdf UniSZA Private Access Isolation and antibiogram of Acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from Lahore, Pakistan International Journal of Biosciences Acinetobacter baumannii is non-fermentive oxygen-consuming Gram-negative coccobacilli pathogen that has appeared as an essential bacteria responsible for hospital-acquired infections. It also has proved itself as an opportunistic pathogen and colonises, especially in those patients which were immunocompromised and were admitted in intensive care units (ICUs), orthopaedic wards, gynae wards and medical wards. The present study was designed to isolate the A.baumannii recovered from different wards of the hospital from various samples. The biochemical characterisation was done by analytical profile index for non-Enterobacteriaceae (API 20NE) system and was subjected for antimicrobial susceptibility pattern. Human clinical specimens like urine, blood, CSF, pus, sputum, HVS and other fluids were examined by the specific methods, and Kirby-Bauer disk diffusion method was adopted according to the Clinical and Laboratory Standards Institute guidelines (CLSI) for the examination of resistivity and susceptibility pattern of pathogens. A total of 150 isolates were recovered out of 912 specimens over the six months (July 2018 to December 2018) in a tertiary care hospital. A high proportion of isolates were resistant against the Ampicillin-Salbactum (69.33%), Cefepime (64.67%), Ceftriaxone (61.33%) and Ceftazidime (60%) whereas Papracillin-Tazobactam (66.67%), Tigecyclin (59.33%), Doxycyclin (56.67%) and Imipenem (55.33%) show maximum efficacy against the isolated pathogens. By adopting the standard policies for the use of antibiotics, increasing resistance of A. baumannii against different classes of drugs could be minimised. 15 5 587-593
spellingShingle Isolation and antibiogram of Acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from Lahore, Pakistan
summary Acinetobacter baumannii is non-fermentive oxygen-consuming Gram-negative coccobacilli pathogen that has appeared as an essential bacteria responsible for hospital-acquired infections. It also has proved itself as an opportunistic pathogen and colonises, especially in those patients which were immunocompromised and were admitted in intensive care units (ICUs), orthopaedic wards, gynae wards and medical wards. The present study was designed to isolate the A.baumannii recovered from different wards of the hospital from various samples. The biochemical characterisation was done by analytical profile index for non-Enterobacteriaceae (API 20NE) system and was subjected for antimicrobial susceptibility pattern. Human clinical specimens like urine, blood, CSF, pus, sputum, HVS and other fluids were examined by the specific methods, and Kirby-Bauer disk diffusion method was adopted according to the Clinical and Laboratory Standards Institute guidelines (CLSI) for the examination of resistivity and susceptibility pattern of pathogens. A total of 150 isolates were recovered out of 912 specimens over the six months (July 2018 to December 2018) in a tertiary care hospital. A high proportion of isolates were resistant against the Ampicillin-Salbactum (69.33%), Cefepime (64.67%), Ceftriaxone (61.33%) and Ceftazidime (60%) whereas Papracillin-Tazobactam (66.67%), Tigecyclin (59.33%), Doxycyclin (56.67%) and Imipenem (55.33%) show maximum efficacy against the isolated pathogens. By adopting the standard policies for the use of antibiotics, increasing resistance of A. baumannii against different classes of drugs could be minimised.
title Isolation and antibiogram of Acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from Lahore, Pakistan
title_full Isolation and antibiogram of Acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from Lahore, Pakistan
title_fullStr Isolation and antibiogram of Acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from Lahore, Pakistan
title_full_unstemmed Isolation and antibiogram of Acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from Lahore, Pakistan
title_short Isolation and antibiogram of Acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from Lahore, Pakistan
title_sort isolation and antibiogram of acinetobacter bauminnii recovered from human clinical specimen in a tertiary care setting from lahore, pakistan