A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat
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| building | INTELEK Repository |
| caption | Advances in Wound Care 2015.4:312-320 |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 |
| date | 2024-08-26 20:53:36 |
| format | Restricted Document |
| id | 12296 |
| institution | UniSZA |
| internalnotes | 1. Shevchenko RV, James SL, James SE. A review of tissue-engineered skin bioconstructs available for skin reconstruction. J R Soc Interface 2010;7:229– 258. 2. Ovington L. Dressing and skin substitute. In: McCulloch J, Kloth L, eds. Wound Healing Evidence-Based Management, 4th ed. Philadelphia, PA: FA Davis, 2010:183–186. 3. Chakrabarty K, Dawson R, Harris P, et al. Development of autologous human dermal–epidermal composites based on sterilized human allodermis for clinical use. Br J Dermatol 1999;141:811–823. 4. Hilmi ABM, Halim AS, Noor NM, et al. A simple culture method for epithelial stem cells derived from human hair follicle. Cent Eur J Biol 2013; 8:432–439. 5. Hilmi M, Bakar A, Halim AS, et al. In vitro characterization of a chitosan skin regenerating template as a scaffold for cells cultivation. Springerplus 2013;2:79. 6. Xiao S, Zhu S, Ma B, et al. A new system for cultivation of human keratinocytes on acellular dermal matrix substitute with the use of human fibroblast feeder layer. Cells Tissues Organs 2007; 187:123–130. 7. Lim CK, Halim AS, Zainol I, Noorsal K. In vitro evaluation of a biomedical-grade bilayer chitosan porous skin regenerating template as a potential dermal scaffold in skin tissue engineering. Int J Polym Sci 2011;2011:Article ID 645820. 8. Mohd Hilmi AB, Halim AS, Jaafar H, Asiah AB, Hassan A. Chitosan dermal substitute and chitosan skin substitute contribute to accelerated fullthickness wound healing in irradiated rats. Biomed Res Int 2013;795458:2013. 9. Biel MA, Kim T, Trump MJ. Effect of radiation therapy and Photofrin on tissue response in a rat model. Lasers Surg Med 1993;13:672–676. 10. Richter GT, Fan CY, Ozgursoy O, McCoy J, Vural E. Effect of vascular endothelial growth factor on skin graft survival in Sprague-Dawley rats. Arch Otolaryngol Head Neck Surg 2006;132:637. 11. Sxakrak T, Ko¨se AA, Kivanc¸ O¨, et al. The effects of combined application of autogenous fibroblast cell culture and full-tissue skin graft (FTSG) on wound healing and contraction in full-thickness tissue defects. Burns 2012;38:225–231. 12. Kubo K, Kuroyanagi Y. A study of cytokines released from fibroblasts in cultured dermal substitute. Artif Organs 2005;29:845–849. 13. Holloway S, Harding K, Stechmiller JK, Schultz G. Acute and chronic wound healing. In: Baranoski S, Ayello EA, eds. Wound Care Essential, 3rd ed. Ambler: Lippincott Williams & Wilkins, 2012:89. 14. Imaizumi N, Monnier Y, Hegi M, Mirimanoff R-O, Ru¨egg C. Radiotherapy suppresses angiogenesis in mice through TGF-bRI/ALK5-dependent inhibition of endothelial cell sprouting. PLoS One 2010;5:e11084. 15. Hardy MA. The biology of scar formation. Phys Ther 1989;69:1014–1024. 16. Mallefet P, Dweck AC. Mechanisms involved in wound healing. Biomed Sci 2008;9:609–615. 17. Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci U S A 1979;76:1274–1278. 18. Bell E, Sher S, Hull B, et al. The reconstitution of living skin. J Invest Dermatol 1983;81(1 Suppl):2s– 10s. 19. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T. Living tissue formed in vitro and accepted as skinequivalent tissue of full thickness. Science 1981; 211:1052–1054. 20. Yannas I, Burke J, Orgill D, Skrabut E. Wound tissue can utilize a polymeric template to synthesize a functional extension of skin. Science 1982;215:174–176. 21. Periayah MH, Halim AS, Hussein AR, et al. In vitro capacity of different grades of chitosan derivatives to induce platelet adhesion and aggregation. Inte J Biol Macromol 2013;52:244–249. 22. Shah Jumaat MY, Ahmad Sukari H, Arman Zaharil MS, Hasnan J. Evaluation of the biocompatibility of a bilayer chitosan skin regenerating template, human skin allograft, and integra implants in rats. ISRN Mater Sci 2011;2011:Article ID 857483. 23. Jeon SJ, Oh M, Yeo W-S, Galva˜o KN, Jeong KC. Underlying mechanism of antimicrobial activity of chitosan microparticles and implications for the treatment of infectious diseases. PLoS One 2014; 9:e92723. 24. Simo˜es M, Ga¨rtner A, Shirosaki Y, et al. In vitro and in vivo chitosan membranes testing for peripheral nerve reconstruction. Acta Me´d Port 2011;24:43–52. 25. Chen B, Bohnert D, Borgens RB, Cho Y. Pushing the science forward: chitosan nanoparticles and functional repair of CNS tissue after spinal cord injury. Injury 2013;5:7–9. 26. Albanna MZ, Bou-Akl TH, Blowytsky O, Walters Iii HL, Matthew HWT. Chitosan fibers with improved biological and mechanical properties for tissue engineering applications. J Mech Behav Biomed Mater 2013;20:217–226. 27. Zhou Y, Yang H, Liu X, et al. Potential of quaternization-functionalized chitosan fiber for wound dressing. Int J Biol Macromol 2013;52:327–332. 28. Brienza DM, Geyer MJ, Sprigle S, Zulkowski K. Pressure redistribution: seating, positioning and support surface. In: Baranoski S, Ayello EA, eds. Wound Care Essentials: Practice Principles, 3rd ed. Philadelphia: Lippincott Williams & Wilkins, 2012:265–294. 29. Skibinski G, Elborn JS, Ennis M. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am J Physiol Lung Cell Mol Physiol 2007;293:L69–L76. 30. Wang Z, Wang Y, Farhangfar F, Zimmer M, Zhang Y. Enhanced keratinocyte proliferation and migration in co-culture with fibroblasts. PLoS One 2012;7:e40951. 31. Keogh MB, O’Brien FJ, Daly JS. A novel collagen scaffold supports human osteogenesis—applications for bone tissue engineering. Cell Tissue Res 2010; 340:169–177. 32. Keck M, Haluza D, Lumenta DB, et al. Construction of a multi-layer skin substitute: simultaneous cultivation of keratinocytes and preadipocytes on a dermal template. Burns 2011;37:626–630. 33. Dai N-T, Yeh M-K, Liu DD, et al. A co-cultured skin model based on cell support membranes. Biochem Biophys Res Commun 2005;329:905–908. 34. Almeida E, Caraca R, Adam R, et al. Photodamage in feline skin: clinical and histomorphometric analysis. Vet Pathol Online 2008;45:327–335. 35. Kozikowski RT, Smith SE, Lee JA, et al. Comparative evaluation of differential laser-induced perturbation spectroscopy as a technique to discriminate emerging skin pathology. J Biomed Opt 2012;17:0670021–06700211. 36. Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci 2004;9:283–289. 37. Guo S, DiPietro LA. Factors affecting wound healing. J Dent Res 2010;89:219–229. 38. Seth AK, De la Garza M, Fang RC, Hong SJ, Galiano RD. Excisional wound healing is delayed in a murine model of chronic kidney disease. PLoS One 2013;8:e59979. 39. Charernsriwilaiwat N, Rojanarata T, Ngawhirunpat T, Sukma M, Opanasopit P. Electrospun chitosan-based nanofiber mats loaded with Garcinia mangostana extracts. Int J Pharm 2013;452: 333–343. 40. Garric X, Guillaume O, Dabboue H, Vert M, Mole`s J-P. Potential of a PLA–PEO–PLA-based scaffold for skin tissue engineering: in vitro evaluation. J Biomater Sci Polym Ed 2012;23: 1687–1700. |
| originalfilename | 6596-01-FH02-FSK-15-03746.pdf |
| person | Mohd Hilmi A.B. Hassan Asma and Halim Ahmad Sukari |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12296 |
| spelling | 12296 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12296 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf Adobe Acrobat Pro DC 20 Paper Capture Plug-in with ClearScan 10 1.6 Mohd Hilmi A.B. Hassan Asma and Halim Ahmad Sukari 2024-08-26 20:53:36 Advances in Wound Care 2015.4:312-320 Advances in Wound Care 2015.4:312-320 6596-01-FH02-FSK-15-03746.pdf UniSZA Private Access A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat Advances in Wound Care Objective: An engineered skin substitute is produced to accelerate wound healing by increasing the mechanical strength of the skin wound via high production of collagen bundles. During the remodeling stage of wound healing, collagen deposition is the most important event. The collagen deposition process may be altered by nutritional deficiency, diabetes mellitus, microbial infection, or radiation exposure, leading to impaired healing. This study describes the fabrication of an engineered bilayer skin substitute and evaluates its effectiveness for the production of collagen bundles in an impaired healing model. Approach: Rats were exposed to 10 Gy of radiation. Two months postirradiation, the wounds were excised and treated with one of three skin replacement products: bilayer engineered skin substitutes, chitosan skin templates, or duodermª. The collagen deposition was analyzed by hematoxylin and eosin staining. Results: On day 21 postwound, the irradiated wounds displayed increased collagen bundle deposition after treatment using bilayer engineered skin substitutes (3.4 – 0.25) and chitosan skin templates (3.2 – 0.58) compared with duoderm (2.0 – 0.63). Innovation: We provide the first report on the fabrication of bilayer engineered skin substitutes using high density human dermal fibroblasts cocultured with HFSCs on chitosan skin templates. Conclusion: The high density of fibroblasts significantly increases the penetration of cells into chitosan skin templates, contributing to the fabrication of bilayer engineered skin substitute. 4 5 312-320 1. Shevchenko RV, James SL, James SE. A review of tissue-engineered skin bioconstructs available for skin reconstruction. J R Soc Interface 2010;7:229– 258. 2. Ovington L. Dressing and skin substitute. In: McCulloch J, Kloth L, eds. Wound Healing Evidence-Based Management, 4th ed. Philadelphia, PA: FA Davis, 2010:183–186. 3. Chakrabarty K, Dawson R, Harris P, et al. Development of autologous human dermal–epidermal composites based on sterilized human allodermis for clinical use. Br J Dermatol 1999;141:811–823. 4. Hilmi ABM, Halim AS, Noor NM, et al. A simple culture method for epithelial stem cells derived from human hair follicle. Cent Eur J Biol 2013; 8:432–439. 5. Hilmi M, Bakar A, Halim AS, et al. In vitro characterization of a chitosan skin regenerating template as a scaffold for cells cultivation. Springerplus 2013;2:79. 6. Xiao S, Zhu S, Ma B, et al. A new system for cultivation of human keratinocytes on acellular dermal matrix substitute with the use of human fibroblast feeder layer. Cells Tissues Organs 2007; 187:123–130. 7. Lim CK, Halim AS, Zainol I, Noorsal K. In vitro evaluation of a biomedical-grade bilayer chitosan porous skin regenerating template as a potential dermal scaffold in skin tissue engineering. Int J Polym Sci 2011;2011:Article ID 645820. 8. Mohd Hilmi AB, Halim AS, Jaafar H, Asiah AB, Hassan A. Chitosan dermal substitute and chitosan skin substitute contribute to accelerated fullthickness wound healing in irradiated rats. Biomed Res Int 2013;795458:2013. 9. Biel MA, Kim T, Trump MJ. Effect of radiation therapy and Photofrin on tissue response in a rat model. Lasers Surg Med 1993;13:672–676. 10. Richter GT, Fan CY, Ozgursoy O, McCoy J, Vural E. Effect of vascular endothelial growth factor on skin graft survival in Sprague-Dawley rats. Arch Otolaryngol Head Neck Surg 2006;132:637. 11. Sxakrak T, Ko¨se AA, Kivanc¸ O¨, et al. The effects of combined application of autogenous fibroblast cell culture and full-tissue skin graft (FTSG) on wound healing and contraction in full-thickness tissue defects. Burns 2012;38:225–231. 12. Kubo K, Kuroyanagi Y. A study of cytokines released from fibroblasts in cultured dermal substitute. Artif Organs 2005;29:845–849. 13. Holloway S, Harding K, Stechmiller JK, Schultz G. Acute and chronic wound healing. In: Baranoski S, Ayello EA, eds. Wound Care Essential, 3rd ed. Ambler: Lippincott Williams & Wilkins, 2012:89. 14. Imaizumi N, Monnier Y, Hegi M, Mirimanoff R-O, Ru¨egg C. Radiotherapy suppresses angiogenesis in mice through TGF-bRI/ALK5-dependent inhibition of endothelial cell sprouting. PLoS One 2010;5:e11084. 15. Hardy MA. The biology of scar formation. Phys Ther 1989;69:1014–1024. 16. Mallefet P, Dweck AC. Mechanisms involved in wound healing. Biomed Sci 2008;9:609–615. 17. Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci U S A 1979;76:1274–1278. 18. Bell E, Sher S, Hull B, et al. The reconstitution of living skin. J Invest Dermatol 1983;81(1 Suppl):2s– 10s. 19. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T. Living tissue formed in vitro and accepted as skinequivalent tissue of full thickness. Science 1981; 211:1052–1054. 20. Yannas I, Burke J, Orgill D, Skrabut E. Wound tissue can utilize a polymeric template to synthesize a functional extension of skin. Science 1982;215:174–176. 21. Periayah MH, Halim AS, Hussein AR, et al. In vitro capacity of different grades of chitosan derivatives to induce platelet adhesion and aggregation. Inte J Biol Macromol 2013;52:244–249. 22. Shah Jumaat MY, Ahmad Sukari H, Arman Zaharil MS, Hasnan J. Evaluation of the biocompatibility of a bilayer chitosan skin regenerating template, human skin allograft, and integra implants in rats. ISRN Mater Sci 2011;2011:Article ID 857483. 23. Jeon SJ, Oh M, Yeo W-S, Galva˜o KN, Jeong KC. Underlying mechanism of antimicrobial activity of chitosan microparticles and implications for the treatment of infectious diseases. PLoS One 2014; 9:e92723. 24. Simo˜es M, Ga¨rtner A, Shirosaki Y, et al. In vitro and in vivo chitosan membranes testing for peripheral nerve reconstruction. Acta Me´d Port 2011;24:43–52. 25. Chen B, Bohnert D, Borgens RB, Cho Y. Pushing the science forward: chitosan nanoparticles and functional repair of CNS tissue after spinal cord injury. Injury 2013;5:7–9. 26. Albanna MZ, Bou-Akl TH, Blowytsky O, Walters Iii HL, Matthew HWT. Chitosan fibers with improved biological and mechanical properties for tissue engineering applications. J Mech Behav Biomed Mater 2013;20:217–226. 27. Zhou Y, Yang H, Liu X, et al. Potential of quaternization-functionalized chitosan fiber for wound dressing. Int J Biol Macromol 2013;52:327–332. 28. Brienza DM, Geyer MJ, Sprigle S, Zulkowski K. Pressure redistribution: seating, positioning and support surface. In: Baranoski S, Ayello EA, eds. Wound Care Essentials: Practice Principles, 3rd ed. Philadelphia: Lippincott Williams & Wilkins, 2012:265–294. 29. Skibinski G, Elborn JS, Ennis M. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am J Physiol Lung Cell Mol Physiol 2007;293:L69–L76. 30. Wang Z, Wang Y, Farhangfar F, Zimmer M, Zhang Y. Enhanced keratinocyte proliferation and migration in co-culture with fibroblasts. PLoS One 2012;7:e40951. 31. Keogh MB, O’Brien FJ, Daly JS. A novel collagen scaffold supports human osteogenesis—applications for bone tissue engineering. Cell Tissue Res 2010; 340:169–177. 32. Keck M, Haluza D, Lumenta DB, et al. Construction of a multi-layer skin substitute: simultaneous cultivation of keratinocytes and preadipocytes on a dermal template. Burns 2011;37:626–630. 33. Dai N-T, Yeh M-K, Liu DD, et al. A co-cultured skin model based on cell support membranes. Biochem Biophys Res Commun 2005;329:905–908. 34. Almeida E, Caraca R, Adam R, et al. Photodamage in feline skin: clinical and histomorphometric analysis. Vet Pathol Online 2008;45:327–335. 35. Kozikowski RT, Smith SE, Lee JA, et al. Comparative evaluation of differential laser-induced perturbation spectroscopy as a technique to discriminate emerging skin pathology. J Biomed Opt 2012;17:0670021–06700211. 36. Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci 2004;9:283–289. 37. Guo S, DiPietro LA. Factors affecting wound healing. J Dent Res 2010;89:219–229. 38. Seth AK, De la Garza M, Fang RC, Hong SJ, Galiano RD. Excisional wound healing is delayed in a murine model of chronic kidney disease. PLoS One 2013;8:e59979. 39. Charernsriwilaiwat N, Rojanarata T, Ngawhirunpat T, Sukma M, Opanasopit P. Electrospun chitosan-based nanofiber mats loaded with Garcinia mangostana extracts. Int J Pharm 2013;452: 333–343. 40. Garric X, Guillaume O, Dabboue H, Vert M, Mole`s J-P. Potential of a PLA–PEO–PLA-based scaffold for skin tissue engineering: in vitro evaluation. J Biomater Sci Polym Ed 2012;23: 1687–1700. |
| spellingShingle | A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat |
| subject | Advances in Wound Care 2015.4:312-320 |
| summary | Objective: An engineered skin substitute is produced to accelerate wound healing by increasing the mechanical strength of the skin wound via high production of collagen bundles. During the remodeling stage of wound healing, collagen deposition is the most important event. The collagen deposition process may be altered by nutritional deficiency, diabetes mellitus, microbial infection, or radiation exposure, leading to impaired healing. This study describes the fabrication of an engineered bilayer skin substitute and evaluates its effectiveness for the production of collagen bundles in an impaired healing model. Approach: Rats were exposed to 10 Gy of radiation. Two months postirradiation, the wounds were excised and treated with one of three skin replacement products: bilayer engineered skin substitutes, chitosan skin templates, or duodermª. The collagen deposition was analyzed by hematoxylin and eosin staining. Results: On day 21 postwound, the irradiated wounds displayed increased collagen bundle deposition after treatment using bilayer engineered skin substitutes (3.4 – 0.25) and chitosan skin templates (3.2 – 0.58) compared with duoderm (2.0 – 0.63). Innovation: We provide the first report on the fabrication of bilayer engineered skin substitutes using high density human dermal fibroblasts cocultured with HFSCs on chitosan skin templates. Conclusion: The high density of fibroblasts significantly increases the penetration of cells into chitosan skin templates, contributing to the fabrication of bilayer engineered skin substitute. |
| title | A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat |
| title_full | A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat |
| title_fullStr | A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat |
| title_full_unstemmed | A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat |
| title_short | A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat |
| title_sort | bilayer engineered skin substitute for wound repair in an irradiation-impeded healing model on rat |