Loop-Mediated Isothermal Amplification (LAMP), an innovation in gene amplification: Bridging the gap in molecular diagnostics; a review
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| internalnotes | 1. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucl Acids Res. 2000; 28:e63. 2. Parida M, Sannarangaiah S, Dash PK, Rao PVL, Morita K. Loop Mediated Isothermal Amplification (LAMP): A new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol. 2008; 18:407–21. 3. Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002; 16:223–9. 4. Dhama K, Karthik K, Chakraborty S, Tiwari R, Kapoor S, Kumar A, Thomas P. Loop-Mediated Isothermal Amplification of DNA (LAMP): A new diagnostic tool lights the world of diagnosis of animal and human pathogens: A review. Pakistan Journal of Biological Sciences. 2014; 17(2):151–66. 5. Compton J. Nucleic acid sequence-based amplification. Nature. 1991; 350(613):91–2. 6. Guatelli JC, Whitfield KM, Kwoh DY, Barringer KJ, Richman DD, Gingeras TR. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proceedings of National Academy Science; USA. 1990. p. 1874–8. 7. Walker GT, Fraiser MS, Schram JL, Little MC, Nadeau JG, Malinowski DP. Strand displacement amplification, an isothermal invitro DNA amplification technique. Nucleic Acid Research.1992; 20(7):1691–6. 8. Lizardi PM, Huang X, Zhu Z, Bray-Ward P, Thomas DC, Ward DC. Mutation detection and single molecule counting using isothermal rolling circle amplification. Nat Genet. 1998; 19:225–32. 9. Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H. Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction. Cold Spring Harb Symp Quant Biol. 1986; 51:263–73. 10. Nagamine K, Watanabe K, Ohtsuka K, Hase T, Notomi T. Loop-mediated isothermal amplification reaction using a non-denatured template. Clin Chem. 2001; 47:1742–3. 11. Nagamine K, Kuzuhara Y, Notomi T. Isolation of single stranded DNA from loop-mediated isothermal amplification products. Biochem Biophys Res Commun. 2002; 290:1195–8. 12. Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun. 2001; 289:150–4. 13. Francois P, Tangomo M, Hibbs J, Bonetti EJ, Boehme CC, et al. Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications. FEMS Immunol Med Microbiol. 2011; 62:41–8. 14. Kaneko H, Iida T, Aoki K, Ohno S, Suzutani T. Sensitive and rapid detection of herpes simplex virus and vericellazoster virus by loop-mediated isothermal amplification. J Clin Microbiol. 2005; 43:3290–6. 15. Tomita N, Mori Y, Kanda H, Notomi T. Loop-Mediated Isothermal Amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc. 2008; 3:877–82. 16. Njiru ZK. Loop-mediated isothermal amplification technology: Towards point of care diagnostics. PLOS Negl Trop Dis. 2012; 6. DOI: 10.1371/journal.pntd.0001572. 17. Parida M, Posadas G, Inoue S, Hasebe F, Morita K. Realtime reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol. 2004; 42:257–63. 18. Parida MM, Horioke K, Ishida H, Dash PK, Saxena P, et al. Rapid detection and differentiation of Dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol. 2005; 43:2895–903. 19. Sen K, Ashbolt NK. Environmental microbiology: Current technology and water, applications. UK: Caister Academic Press; 2010. 20. Mori Y, Kitao M, Tomita N, Notomi T. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods. 2004; 59:145–57. 21. Goto M, Honda E, Ogura A, Nomoto A, Hanaki K. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxynaphthol blue. Biotechniques. 2009; 46:167–72. 22. Cutis KA, Rudolph DL, Omen SM. Rapid detection of HIV-1 by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP). J Virol Methods. 2008; 151:264–70. 23. Dukes JP, King DP, Alexanderson S. Novel reverse transcription Loop-mediated isothermal amplification for rapid and detection of foot- and -mouth disease virus. Arch Virol. 2006; 151:1093–106. 24. Thekisoe OM, Kuboki N, Nambota A, Fujisaki K, Sugimoto C, et al. Species-specific Loop-Mediated Isothermal Amplification (LAMP) for diagnosis of trypanosomosis. Acta Tropica. 2007; 102:182–9. 25. Hagiwara M, Sasaki H, Matsuo K, Honda M, Kawase M, Nakagawa H. Loop-Mediated Isothermal Amplification method for detection of human papillomavirus type 6, 11, 16 and 18. J Med Virol. 2007; 79:605–15. 26. Xia JF, Yan XF, Yu H, Qu D, Long JE. Simple and rapid detection of human enterovirus 71 by reverse-transcription and loop-mediated isothermal amplification: Cryopreservation affected the detection ability. Diagn Microbiol Infect Dis. 2011; 71:244–51. 27. Ihira M, Akimoto S, Miyake F, Fujita A, Sugata K, et al. Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method. J Clin Virol. 2007; 39:22–6. 28. Wakabayashi T, Yamashita R, Kakita T, et al. Rapid detection of Adenoviral keratoconjunctivitis by Loop-Mediated Isothermal Amplification (LAMP) method. Curr Eye Res. 2004; 128(6):445–50. 29. Kubo T, Agoh M, Le Mai Q, Fukushima K, Nishimura H, et al. Development of a reverse transcription-loop-mediated isothermal amplification assay for detection of pandemic (H1N1) 2009 virus as a novel molecular method for diagnosis of pandemic influenza in resource-limited settings. J Clin Microbiol. 2010; 48:728–35. 30. Wozniakowski G. KozdrunW, Samorek-Salamonowicz E. 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Rapid and specific detection of Escherichia coli sero groups O26, O45, O103, O111, O121, O145 and O157 in ground beef, beef trim and produce by loop-mediated isothermal amplification. Applied Environ Microbiol. 2012; 78:2727–36. 40. Sonthayanon P, Chierakul W, Wuthiekanun V, Thaipadungpanit J, Kalambaheti T, et al. Accuracy of loopmediated isothermal amplification for diagnosis of human leptospirosis in Thailand. Am J Trop Med Hygiene. 2011; 84:614–20. 41. Suzuki R, Yoshikawa T, Ihira M, Enomoto Y, Inagaki S, Matsumoto K, Kato K, Kudo K, Kojima S, Asano S. Development of the loop-mediated isothermal amplification method for rapid detection of cytomegalovirus DNA. Journal of Virological Methods. 2007; 132(1):216–21. 42. Song L, Li J, Hou S, Li X, Chen S. Establishment of LoopMediated Isothermal Amplification (LAMP) for rapid detection of Brucella spp. and application to milk and blood samples. J Microbiol Met. 2012; 90:292–7. 43. Poon LLM, Wong BWY, Ma EHT, Chan KH, Chow LMC, et al. Sensitive and inexpensive molecular test for Falciparum malaria: Detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. 2006; 52:303–6. 44. Badolo A, Okado K, Guelbeogo WM, Aonuma H, Bando H, Fukumoto S, Sagnnon S, Kanuka H. Development of an allele-specific Loop-Mediated Isothermal Amplification (AS-LAMP) to detect the L1014F Kdr-W mutation in Anopheles gambiae S.I. Malaria Journal. 2012; 11:227. 45. Dinh DT, Le MTQ, Vuong CD, Hasebe F, Morita K. An updated loop-mediated isothermal amplification method for rapid diagnosis of H5N1 Avian Influenza Viruses. Trop Med Health. 2011; 39:3–7. 46. Uemura N, Makimura K, Onozaki M, Otsuka Y, Shibuya Y, et al. Development of a loop-mediated isothermal amplification method for diagnosing Pneumocystis pneumonia. J Med Microbiol. 2008; 57:50–7. 47. Inacio J, Flores O, Spencer-Martins I. 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The Loop-mediated isothermal amplification assay for rapid diagnosis of Bebesia canis canis infection in dogs. Polish Journal of Veterinary Sciences. 2013; 16(1):131–3. 97. Salant H, Abbassi I, Hamburger J. The development of a Loop-Mediated Isothermal Amplification Method (LAMP) for Echinococcus granulosus [corrected] coprodetection. Am J Trop Med Hygiene. 2012; 87(5):883–7. |
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| spelling | 12266 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12266 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 767 96 96 1420 88 88 2015-09-03 10:22:14 1420x767 6566-01-FH02-FP-15-03715.jpg UniSZA Private Access Loop-Mediated Isothermal Amplification (LAMP), an innovation in gene amplification: Bridging the gap in molecular diagnostics; a review Indian Journal of Science and Technology Loop-Mediated Isothermal Amplification (LAMP), through its uniqueness has provided the world with a powerful molecular diagnostic tool. This innovative gene amplification technique, amplifies nucleic acid at a very rapid pace, maintaining high sensitivity, specificity and efficiency. The most innovative features of LAMP, is the simplicity of its protocol, and overall low cost of application. Other features of LAMP are highlighted in this review, though with few limitations. The demerits include highly technical and cumbersome primer design protocol, and relative scarcity of some materials needed for the technique. The standout features however, ranges from the strand displacement activity of the Bst and Bsm polymerase enzymes, to its ability to amplify gene in an unprocessed samples. The PCR and other several pre-existing techniques, has contributed immensely in disease diagnosis and molecular biology at large. However, their use in molecular diagnostics is mostly limited to highly resourced establishment, leaving the low resourced settings with little or no hope. The cheapness and user-friendliness of LAMP amongst other advantages made it an ideal diagnostic tool that provides solution to the odds of PCR and its likes. The outstanding qualities displayed by LAMP in various researches, has led to several modifications in the technique. Presently several forms of LAMP kits, such as Lyophilized LAMP Kit, electric LAMP device (eLAMP), and combined use of LAMP and Lateral Flow Assay (LFA), are been adopted for diagnosis of many pathogens. 8 17 Indian Society for Education and Environment Indian Society for Education and Environment 1-12 1. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucl Acids Res. 2000; 28:e63. 2. Parida M, Sannarangaiah S, Dash PK, Rao PVL, Morita K. Loop Mediated Isothermal Amplification (LAMP): A new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol. 2008; 18:407–21. 3. Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002; 16:223–9. 4. Dhama K, Karthik K, Chakraborty S, Tiwari R, Kapoor S, Kumar A, Thomas P. Loop-Mediated Isothermal Amplification of DNA (LAMP): A new diagnostic tool lights the world of diagnosis of animal and human pathogens: A review. Pakistan Journal of Biological Sciences. 2014; 17(2):151–66. 5. Compton J. Nucleic acid sequence-based amplification. Nature. 1991; 350(613):91–2. 6. Guatelli JC, Whitfield KM, Kwoh DY, Barringer KJ, Richman DD, Gingeras TR. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proceedings of National Academy Science; USA. 1990. p. 1874–8. 7. Walker GT, Fraiser MS, Schram JL, Little MC, Nadeau JG, Malinowski DP. Strand displacement amplification, an isothermal invitro DNA amplification technique. Nucleic Acid Research.1992; 20(7):1691–6. 8. Lizardi PM, Huang X, Zhu Z, Bray-Ward P, Thomas DC, Ward DC. Mutation detection and single molecule counting using isothermal rolling circle amplification. Nat Genet. 1998; 19:225–32. 9. Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H. Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction. Cold Spring Harb Symp Quant Biol. 1986; 51:263–73. 10. Nagamine K, Watanabe K, Ohtsuka K, Hase T, Notomi T. Loop-mediated isothermal amplification reaction using a non-denatured template. Clin Chem. 2001; 47:1742–3. 11. Nagamine K, Kuzuhara Y, Notomi T. Isolation of single stranded DNA from loop-mediated isothermal amplification products. Biochem Biophys Res Commun. 2002; 290:1195–8. 12. Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun. 2001; 289:150–4. 13. Francois P, Tangomo M, Hibbs J, Bonetti EJ, Boehme CC, et al. Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications. FEMS Immunol Med Microbiol. 2011; 62:41–8. 14. Kaneko H, Iida T, Aoki K, Ohno S, Suzutani T. Sensitive and rapid detection of herpes simplex virus and vericellazoster virus by loop-mediated isothermal amplification. J Clin Microbiol. 2005; 43:3290–6. 15. Tomita N, Mori Y, Kanda H, Notomi T. Loop-Mediated Isothermal Amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc. 2008; 3:877–82. 16. Njiru ZK. Loop-mediated isothermal amplification technology: Towards point of care diagnostics. PLOS Negl Trop Dis. 2012; 6. DOI: 10.1371/journal.pntd.0001572. 17. Parida M, Posadas G, Inoue S, Hasebe F, Morita K. Realtime reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol. 2004; 42:257–63. 18. Parida MM, Horioke K, Ishida H, Dash PK, Saxena P, et al. Rapid detection and differentiation of Dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol. 2005; 43:2895–903. 19. Sen K, Ashbolt NK. Environmental microbiology: Current technology and water, applications. UK: Caister Academic Press; 2010. 20. Mori Y, Kitao M, Tomita N, Notomi T. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods. 2004; 59:145–57. 21. Goto M, Honda E, Ogura A, Nomoto A, Hanaki K. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxynaphthol blue. Biotechniques. 2009; 46:167–72. 22. Cutis KA, Rudolph DL, Omen SM. Rapid detection of HIV-1 by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP). J Virol Methods. 2008; 151:264–70. 23. Dukes JP, King DP, Alexanderson S. Novel reverse transcription Loop-mediated isothermal amplification for rapid and detection of foot- and -mouth disease virus. Arch Virol. 2006; 151:1093–106. 24. Thekisoe OM, Kuboki N, Nambota A, Fujisaki K, Sugimoto C, et al. Species-specific Loop-Mediated Isothermal Amplification (LAMP) for diagnosis of trypanosomosis. 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| spellingShingle | Loop-Mediated Isothermal Amplification (LAMP), an innovation in gene amplification: Bridging the gap in molecular diagnostics; a review |
| summary | Loop-Mediated Isothermal Amplification (LAMP), through its uniqueness has provided the world with a powerful molecular diagnostic tool. This innovative gene amplification technique, amplifies nucleic acid at a very rapid pace, maintaining high sensitivity, specificity and efficiency. The most innovative features of LAMP, is the simplicity of its protocol, and overall low cost of application. Other features of LAMP are highlighted in this review, though with few limitations. The demerits include highly technical and cumbersome primer design protocol, and relative scarcity of some materials needed for the technique. The standout features however, ranges from the strand displacement activity of the Bst and Bsm polymerase enzymes, to its ability to amplify gene in an unprocessed samples. The PCR and other several pre-existing techniques, has contributed immensely in disease diagnosis and molecular biology at large. However, their use in molecular diagnostics is mostly limited to highly resourced establishment, leaving the low resourced settings with little or no hope. The cheapness and user-friendliness of LAMP amongst other advantages made it an ideal diagnostic tool that provides solution to the odds of PCR and its likes. The outstanding qualities displayed by LAMP in various researches, has led to several modifications in the technique. Presently several forms of LAMP kits, such as Lyophilized LAMP Kit, electric LAMP device (eLAMP), and combined use of LAMP and Lateral Flow Assay (LFA), are been adopted for diagnosis of many pathogens. |
| title | Loop-Mediated Isothermal Amplification (LAMP), an innovation in gene amplification: Bridging the gap in molecular diagnostics; a review |
| title_full | Loop-Mediated Isothermal Amplification (LAMP), an innovation in gene amplification: Bridging the gap in molecular diagnostics; a review |
| title_fullStr | Loop-Mediated Isothermal Amplification (LAMP), an innovation in gene amplification: Bridging the gap in molecular diagnostics; a review |
| title_full_unstemmed | Loop-Mediated Isothermal Amplification (LAMP), an innovation in gene amplification: Bridging the gap in molecular diagnostics; a review |
| title_short | Loop-Mediated Isothermal Amplification (LAMP), an innovation in gene amplification: Bridging the gap in molecular diagnostics; a review |
| title_sort | loop-mediated isothermal amplification (lamp), an innovation in gene amplification: bridging the gap in molecular diagnostics; a review |