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1860797279110168576
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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| date |
2019-03-25 17:22:54
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| format |
Restricted Document
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12060
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UniSZA
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6363-01-FH02-FSK-19-28688.pdf
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Adobe InDesign CC 14.0 (Windows)
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12060
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12060 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=12060 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 1.7 Adobe InDesign CC 14.0 (Windows) 2019-03-25 17:22:54 xmp.id:891d5dbf-d902-bc40-896a-7b761c9f20f5 6363-01-FH02-FSK-19-28688.pdf UniSZA Private Access Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis EC Microbiology Objective: The purpose of the present study was to assess the effects of media, optical density, and incubation time on biofilm formation by Staphylococcus epidermidis. Methods: The main procedure involved was fixing the bacterial film with 95% ethanol, staining with 0.1% crystal violet, releasing the bound dye with 33% glacial acetic acid, and measuring the optical density (OD) of the solution at 590 nm by using a microplate reader. Results: It was found that 3 and 5 days of incubation are critical for biofilm formation as indicated by the OD values of 0.55 - 0.06 and 0.70 - 0.39 in MHB and TSB respectively at OD 0.05. Similar results were noted for OD 0.1 in both media MHB and TSB while TSB showing slightly better results for biofilm formation. Conclusion: It is concluded that three days (72 hours) are required for obtaining effective biofilm formation in both MHB and TSB at 370C at OD 0.05 and OD 0.1. 15 4 277-282
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Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis
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| summary |
Objective: The purpose of the present study was to assess the effects of media, optical density, and incubation time on biofilm formation by Staphylococcus epidermidis. Methods: The main procedure involved was fixing the bacterial film with 95% ethanol, staining with 0.1% crystal violet, releasing the bound dye with 33% glacial acetic acid, and measuring the optical density (OD) of the solution at 590 nm by using a microplate reader. Results: It was found that 3 and 5 days of incubation are critical for biofilm formation as indicated by the OD values of 0.55 - 0.06 and 0.70 - 0.39 in MHB and TSB respectively at OD 0.05. Similar results were noted for OD 0.1 in both media MHB and TSB while TSB showing slightly better results for biofilm formation. Conclusion: It is concluded that three days (72 hours) are required for obtaining effective biofilm formation in both MHB and TSB at 370C at OD 0.05 and OD 0.1.
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| title |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis
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| title_full |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis
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| title_fullStr |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis
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| title_full_unstemmed |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis
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| title_short |
Effect of growth media and optical density on biofilm formation by Staphylococcus epidermidis
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| title_sort |
effect of growth media and optical density on biofilm formation by staphylococcus epidermidis
|